PMID- 30114378
OWN - NLM
STAT- MEDLINE
DCOM- 20191209
LR  - 20220409
IS  - 1840-4812 (Electronic)
IS  - 1512-8601 (Print)
IS  - 1512-8601 (Linking)
VI  - 19
IP  - 2
DP  - 2019 May 20
TI  - Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary 
      thyroid cancer (PTC).
PG  - 146-154
LID - 10.17305/bjbms.2018.2865 [doi]
AB  - Previous studies demonstrated dysregulation of different microRNAs in thyroid 
      cancer. Tetraspanins (TSPANs) are cell surface proteins with critical roles in 
      many cellular processes, and implications in tumor development. Here we 
      investigated the role of miR-369-3p in papillary thyroid cancer (PTC) and its 
      association with TSPAN13. miR-369-3p and the TSPAN13 gene expression profiles of 
      513 thyroid cancer and 59 normal thyroid tissues were downloaded from the Cancer 
      Genome Atlas database. Thyroid cancer tissues were classified according to the 
      histological type, grouped based on low and high median miR-369-3p and TSPAN13 
      expression, and analyzed in relation to overall survival (OS) of patients. Human 
      PTC cell lines (TPC-1 and GLAG-66) and human embryonic kidney 293T (HEK293T) 
      cells were used for in vitro analysis. Transfection experiments were performed 
      with synthetic miRNA mimics for miR-369-3p and small interfering RNAs for 
      TSPAN13. Relative expression of miR-369-3p and TSPAN13 mRNA was determined by 
      RT-qPCR. Protein levels of TSPAN13 were determined by western blotting. Cell 
      proliferation (CCK-8 assay), colony formation, and apoptosis (flow cytometry) 
      were analyzed in transfected cells. Binding sites of miR-369-3p in TSPAN13 mRNA 
      were determined by bioinformatics analysis and dual luciferase reporter assay. 
      miR-369-3p was downregulated and TSPAN13 upregulated in PTC, follicular thyroid 
      cancer, and tall cell variant tissues. Both low expression of miR-369-3p and high 
      expression of TSPAN13 were associated with shorter OS in thyroid cancer patients. 
      Overexpression of miR-369-3p significantly suppressed proliferation and promoted 
      apoptosis in PTC cells. TSPAN13 was a direct target of miR-369-3p, and silencing 
      of TSPAN13 phenocopied the effect of miR-369-3p mimics in PTC cells. Overall, the 
      downregulation of miR-369-3p and consequent upregulation of its target TSPAN13 
      appear to be involved in pathophysiology of PTC.
FAU - Li, Peng
AU  - Li P
AD  - Department of Emergency, Jingmen NO.1 People's Hospital, Jingmen, Hubei, China. 
      peng_li1983@126.com.
FAU - Dong, Mingqiang
AU  - Dong M
FAU - Wang, Zhigang
AU  - Wang Z
LA  - eng
PT  - Journal Article
DEP - 20190520
PL  - Bosnia and Herzegovina
TA  - Bosn J Basic Med Sci
JT  - Bosnian journal of basic medical sciences
JID - 101200947
RN  - 0 (MIRN369 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (TSPAN13 protein, human)
RN  - 0 (Tetraspanins)
SB  - IM
MH  - Apoptosis
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Computational Biology
MH  - *Down-Regulation
MH  - Epigenesis, Genetic
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - HEK293 Cells
MH  - Humans
MH  - MicroRNAs/*genetics/metabolism
MH  - Tetraspanins/*metabolism
MH  - Thyroid Cancer, Papillary/genetics/*pathology
MH  - Treatment Outcome
PMC - PMC6535391
EDAT- 2018/08/17 06:00
MHDA- 2019/12/18 06:00
PMCR- 2019/05/01
CRDT- 2018/08/17 06:00
PHST- 2017/12/20 00:00 [received]
PHST- 2018/01/30 00:00 [accepted]
PHST- 2018/08/17 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2018/08/17 06:00 [entrez]
PHST- 2019/05/01 00:00 [pmc-release]
AID - BJBMS-19-146 [pii]
AID - 10.17305/bjbms.2018.2865 [doi]
PST - epublish
SO  - Bosn J Basic Med Sci. 2019 May 20;19(2):146-154. doi: 10.17305/bjbms.2018.2865.