PMID- 30115049
OWN - NLM
STAT- MEDLINE
DCOM- 20181108
LR  - 20230928
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 14
IP  - 1
DP  - 2018 Aug 16
TI  - Oral administration of inactivated porcine epidemic diarrhea virus activate DCs 
      in porcine Peyer's patches.
PG  - 239
LID - 10.1186/s12917-018-1568-z [doi]
LID - 239
AB  - BACKGROUND: Peyer's patches (PPs) can be considered as the immune site of the 
      intestine. Within PPs, Dendritic cells (DCs) can uptake antigens from the gut 
      lumen by extending dendrites into epithelium, and process it and then present to 
      lymphocytes, which effectively antigen produces an immune response. Porcine 
      epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic 
      diarrhea (PED), an acute and highly contagious enteric viral disease. The 
      interaction between inactivated porcine epidemic diarrhea virus and porcine 
      monocyte-derived dendritic cells (Mo-DCs) has been reported. However, little is 
      known about the interaction between inactivated PEDV and DCs in porcine PPs. 
      RESULTS: In this study, for the first time we investigated the role of DCs in 
      porcine PPs after oral administration inactivated PEDV. Firstly, a method to 
      isolate DCs from porcine PPs was established, in which the purity of 
      SWC3a(+)/MHC-II(+) DCs was more than 90%. Our findings clearly indicate that DCs 
      in porcine PPs after oral administration of inactivated PEDV not only stimulated 
      the proliferation of allogeneic lymphocytes, but also secreted cytokines (IL-1, 
      IL-4). Furthermore, the number of DCs and IgA(+) cells in porcine intestinal 
      mucosal significantly increased and the levels of anti-PEDV specific IgG antibody 
      in the serum and SIgA antibody in the feces increased after oral administration 
      inactivated PEDV. CONCLUSIONS: Our findings indicate that oral administration of 
      inactivated PEDV activate DCs in porcine Peyer's patches and inactivated PEDV may 
      be a useful and safe vaccine to trigger adaptive immunity.
FAU - Yuan, Chen
AU  - Yuan C
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of veterinary medicine, Nanjing Agricultural University, Weigang 1, 
      Nanjing, Jiangsu, 210095, People's Republic of China.
FAU - Zhang, En
AU  - Zhang E
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of veterinary medicine, Nanjing Agricultural University, Weigang 1, 
      Nanjing, Jiangsu, 210095, People's Republic of China.
FAU - Huang, Lulu
AU  - Huang L
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of veterinary medicine, Nanjing Agricultural University, Weigang 1, 
      Nanjing, Jiangsu, 210095, People's Republic of China.
FAU - Wang, Jialu
AU  - Wang J
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of veterinary medicine, Nanjing Agricultural University, Weigang 1, 
      Nanjing, Jiangsu, 210095, People's Republic of China.
FAU - Yang, Qian
AU  - Yang Q
AD  - MOE Joint International Research Laboratory of Animal Health and Food Safety, 
      College of veterinary medicine, Nanjing Agricultural University, Weigang 1, 
      Nanjing, Jiangsu, 210095, People's Republic of China. zxbyq@njau.edu.cn.
LA  - eng
GR  - 31772777/National Science Grant of China/
PT  - Journal Article
DEP - 20180816
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Vaccines)
SB  - IM
EIN - BMC Vet Res. 2018 Oct 3;14(1):303. PMID: 30285740
MH  - Administration, Oral
MH  - Animals
MH  - Coronavirus Infections/immunology/*veterinary/virology
MH  - Dendritic Cells/*immunology
MH  - Intestinal Mucosa/immunology
MH  - Male
MH  - Peyer's Patches/*immunology
MH  - Porcine epidemic diarrhea virus/*immunology/radiation effects
MH  - Sus scrofa
MH  - Swine
MH  - Swine Diseases/*immunology/virology
MH  - Ultraviolet Rays
MH  - Vaccines, Inactivated/immunology
MH  - Viral Vaccines/immunology
PMC - PMC6097195
OTO - NOTNLM
OT  - Dendritic cells
OT  - Inactivated porcine epidemic diarrhea virus
OT  - Peyer's patches
COIS- ETHICS APPROVAL: All efforts were made to minimize suffering. The animal protocol 
      was approved by the University of Nanjing Agriculture University Committee on 
      Animal Resources Committee (Permit Number: SYXK2011-0036). CONSENT FOR 
      PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they 
      have no competing interests. PUBLISHER'S NOTE: Springer Nature remains neutral 
      with regard to jurisdictional claims in published maps and institutional 
      affiliations.
EDAT- 2018/08/18 06:00
MHDA- 2018/11/09 06:00
PMCR- 2018/08/16
CRDT- 2018/08/18 06:00
PHST- 2018/05/20 00:00 [received]
PHST- 2018/08/09 00:00 [accepted]
PHST- 2018/08/18 06:00 [entrez]
PHST- 2018/08/18 06:00 [pubmed]
PHST- 2018/11/09 06:00 [medline]
PHST- 2018/08/16 00:00 [pmc-release]
AID - 10.1186/s12917-018-1568-z [pii]
AID - 1568 [pii]
AID - 10.1186/s12917-018-1568-z [doi]
PST - epublish
SO  - BMC Vet Res. 2018 Aug 16;14(1):239. doi: 10.1186/s12917-018-1568-z.