PMID- 30116142
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2018
DP  - 2018
TI  - Folic Acid Improves the Inflammatory Response in LPS-Activated THP-1 Macrophages.
PG  - 1312626
LID - 10.1155/2018/1312626 [doi]
LID - 1312626
AB  - DNA methylation has been suggested as a regulatory mechanism behind some 
      inflammatory processes. The physiological actions of methyl donors, such as folic 
      acid, choline, and vitamin B(12) on inflammation-related disease have been 
      associated with the synthesis of the universal methyl donor S-adenosyl methionine 
      (SAM). The aim of this study was to evaluate the effects of folic acid, choline, 
      vitamin B(12), and a combination of all on preventing the lipopolysaccharide- 
      (LPS-) induced inflammatory response in human THP-1 monocyte/macrophage cells. 
      Folic acid and the mixture of methyl donors reduced interleukin 1 beta (IL1B) and 
      tumour necrosis factor (TNF) expression as well as protein secretion by these 
      cells. Folic acid and choline decreased C-C motif chemokine ligand 2 (CCL2) mRNA 
      levels. In addition to this, the methyl donor mixture reduced Cluster of 
      differentiation 40 (CD40) expression, but increased serpin family E member 1 
      (SERPINE1) expression. All methyl donors increased methylation levels in CpGs 
      located in IL1B, SERPINE1, and interleukin 18 (IL18) genes. However, TNF 
      methylation was not modified. After treatment with folic acid and the methyl 
      donor mixture, ChIP analysis showed no change in the binding affinity of nuclear 
      factor-kappaB (NF-kappaB) to IL1B and TNF promoter regions after the treatment with folic 
      acid and the methyl donor mixture. The findings of this study suggest that folic 
      acid might contribute to the control of chronic inflammation in 
      inflammatory-related disease.
FAU - Samblas, Mirian
AU  - Samblas M
AUID- ORCID: 0000-0003-0866-2300
AD  - Department of Nutrition, Food Science, and Physiology, Centre for Nutrition 
      Research, University of Navarra, Pamplona, Spain.
AD  - Centro de Investigacion Biomedica en Red de la Fisiopatologia de la Obesidad y 
      Nutricion (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.
FAU - Martinez, J Alfredo
AU  - Martinez JA
AUID- ORCID: 0000-0001-5218-6941
AD  - Department of Nutrition, Food Science, and Physiology, Centre for Nutrition 
      Research, University of Navarra, Pamplona, Spain.
AD  - Centro de Investigacion Biomedica en Red de la Fisiopatologia de la Obesidad y 
      Nutricion (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.
AD  - Instituto de Investigacion Sanitaria de Navarra (IdiSNA), Pamplona, Spain.
AD  - IMDEA Food, Madrid, Spain.
FAU - Milagro, Fermin
AU  - Milagro F
AUID- ORCID: 0000-0002-3228-9916
AD  - Department of Nutrition, Food Science, and Physiology, Centre for Nutrition 
      Research, University of Navarra, Pamplona, Spain.
AD  - Centro de Investigacion Biomedica en Red de la Fisiopatologia de la Obesidad y 
      Nutricion (CIBERobn), Instituto de Salud Carlos III, Madrid, Spain.
LA  - eng
PT  - Journal Article
DEP - 20180704
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 935E97BOY8 (Folic Acid)
RN  - N91BDP6H0X (Choline)
RN  - P6YC3EG204 (Vitamin B 12)
SB  - IM
MH  - Cell Survival
MH  - Choline/pharmacology
MH  - CpG Islands
MH  - DNA Methylation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Folic Acid/*pharmacology
MH  - Humans
MH  - *Inflammation
MH  - Lipopolysaccharides
MH  - Macrophages/*drug effects/metabolism
MH  - NF-kappa B/metabolism
MH  - Promoter Regions, Genetic
MH  - Protein Binding
MH  - THP-1 Cells/*cytology
MH  - Vitamin B 12/pharmacology
PMC - PMC6079441
EDAT- 2018/08/18 06:00
MHDA- 2018/12/12 06:00
PMCR- 2018/07/04
CRDT- 2018/08/18 06:00
PHST- 2018/01/16 00:00 [received]
PHST- 2018/04/19 00:00 [revised]
PHST- 2018/05/16 00:00 [accepted]
PHST- 2018/08/18 06:00 [entrez]
PHST- 2018/08/18 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/07/04 00:00 [pmc-release]
AID - 10.1155/2018/1312626 [doi]
PST - epublish
SO  - Mediators Inflamm. 2018 Jul 4;2018:1312626. doi: 10.1155/2018/1312626. 
      eCollection 2018.