PMID- 30116377
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 16
IP  - 2
DP  - 2018 Aug
TI  - Curcumin inhibits proliferation and promotes apoptosis of breast cancer cells.
PG  - 1266-1272
LID - 10.3892/etm.2018.6345 [doi]
AB  - Curcumin is a natural compound that appears to be promising for clinical 
      application, as it has been shown in in vitro and in vivo studies to exert 
      antitumor effects by modulating multiple signaling cellular pathways. In the 
      present study, the antitumor effects of curcumin and its mechanism of action were 
      investigated in cultured breast cancer cells. The MTT assay was used to determine 
      the effect of curcumin on breast cancer cell proliferation, flow cytometry was 
      used to detect alterations of the cell cycle, and western blot analysis was used 
      to determine the expression of signaling molecules involved in the cell cycle, 
      proliferation and apoptosis. The results revealed that curcumin significantly 
      inhibited the proliferation of various breast cancer cell lines, such as T47D, 
      MCF7, MDA-MB-231 and MDA-MB-468, with an IC(50) at the micromolar level, 
      indicating the potent antitumor activity of curcumin. In-depth study of its 
      mechanism of action revealed that curcumin induced cell cycle arrest at the G2/M 
      phase and decreased the expression of the CDC25 and CDC2 proteins, while 
      increasing the expression of P21. In addition, curcumin inhibited the 
      phosphorylation of protein kinase B (Akt)/mammalian target of rapamycin (mTOR), 
      decreased B-cell lymphoma 2 (BCL2) and promoted BCL-2-associated X protein (BAX) 
      and cleavage of caspase 3, subsequently inducing apoptosis of breast cancer 
      cells. In conclusion, curcumin inhibited the proliferation of breast cancer cells 
      and induced G2/M phase cell cycle arrest and apoptosis, which may be associated 
      with the decrease of CDC25 and CDC2 and increase of P21 protein levels, as well 
      as inhibition of the phosphorylation of Akt/mTOR and induction of the 
      mitochondrial apoptotic pathway. The findings of the present study may provide a 
      basis for the further study of curcumin in the treatment of breast cancer.
FAU - Hu, Shan
AU  - Hu S
AD  - Department of Breast and Thyroid Surgery, Shaoxing People's Hospital, Shaoxing 
      Hospital of Zhejiang University, Shaoxing, Zhejiang 312000, P.R. China.
FAU - Xu, Yingchun
AU  - Xu Y
AD  - Department of Breast and Thyroid Surgery, Shaoxing People's Hospital, Shaoxing 
      Hospital of Zhejiang University, Shaoxing, Zhejiang 312000, P.R. China.
FAU - Meng, Liwei
AU  - Meng L
AD  - Department of Breast and Thyroid Surgery, Shaoxing People's Hospital, Shaoxing 
      Hospital of Zhejiang University, Shaoxing, Zhejiang 312000, P.R. China.
FAU - Huang, Liming
AU  - Huang L
AD  - Department of Breast and Thyroid Surgery, Shaoxing People's Hospital, Shaoxing 
      Hospital of Zhejiang University, Shaoxing, Zhejiang 312000, P.R. China.
FAU - Sun, He
AU  - Sun H
AD  - Clinical Laboratory Center, Shaoxing People's Hospital, Shaoxing Hospital of 
      Zhejiang University, Shaoxing, Zhejiang 312000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180622
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6090267
OTO - NOTNLM
OT  - cell apoptosis
OT  - cell cycle
OT  - curcumin
OT  - signaling pathway
EDAT- 2018/08/18 06:00
MHDA- 2018/08/18 06:01
PMCR- 2018/06/22
CRDT- 2018/08/18 06:00
PHST- 2017/12/27 00:00 [received]
PHST- 2018/03/29 00:00 [accepted]
PHST- 2018/08/18 06:00 [entrez]
PHST- 2018/08/18 06:00 [pubmed]
PHST- 2018/08/18 06:01 [medline]
PHST- 2018/06/22 00:00 [pmc-release]
AID - ETM-0-0-6345 [pii]
AID - 10.3892/etm.2018.6345 [doi]
PST - ppublish
SO  - Exp Ther Med. 2018 Aug;16(2):1266-1272. doi: 10.3892/etm.2018.6345. Epub 2018 Jun 
      22.