PMID- 30116759
OWN - NLM
STAT- MEDLINE
DCOM- 20181105
LR  - 20181114
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2018
DP  - 2018
TI  - Construction and Characterization of Adenovirus Vectors Encoding 
      Aspartate-beta-Hydroxylase to Preliminary Application in Immunotherapy of 
      Hepatocellular Carcinoma.
PG  - 9832467
LID - 10.1155/2018/9832467 [doi]
LID - 9832467
AB  - Dendritic cells (DCs) harboring tumor-associated antigen are supposed to be a 
      potential immunotherapy for hepatocellular carcinoma (HCC). 
      Aspartate-beta-hydroxylase (AAH), an overexpressed tumor-associated cell surface 
      protein, is considered as a promising biomarker and therapeutic target for HCC. 
      In this study, we constructed adenovirus vector encoding AAH gene by gateway 
      recombinant cloning technology and preliminarily explored the antitumor effects 
      of DC vaccines harboring AAH. Firstly, the total AAH mRNA was extracted from 
      human HCC tissues; the cDNA was amplified by RT-PCR, verified, and sequenced 
      after TA cloning. Gateway technology was used and the obtained 18T-AAH was used 
      as a substrate, to yield the final expression vector Ad-AAH-IRES2-EGFP. Secondly, 
      bone marrow-derived DCs were infected by Ad-AAH-IRES2-EGFP to yield AAH-DC 
      vaccines. Matured DCs were demonstrated by increased expression of CD11c, CD80, 
      and MHC-II costimulatory molecules. A dramatically cell-killing effect of T 
      lymphocytes coculturing with AAH-DCs on HepG2 HCC cell line was demonstrated by 
      CCK-8 and FCM assays in vitro. More importantly, in an animal experiment, the 
      lysis effect of cytotoxic T lymphocytes (CTLs) on HepG2 cells in the AAH-DC group 
      was stronger than that in the control groups. In conclusion, the gateway 
      recombinant cloning technology is a powerful method of constructing adenovirus 
      vector, and the product Ad-AAH-IRES2-EGFP may present as a potential candidate 
      for DC-based immunotherapy of HCC.
FAU - Zhou, Yujiao
AU  - Zhou Y
AUID- ORCID: 0000-0002-3328-3153
AD  - Department of Infectious Disease, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, China.
FAU - Liu, Feifei
AU  - Liu F
AD  - Department of Infectious Disease, The People's Hospital of Deyang City, Deyang, 
      Sichuan Province 618000, China.
FAU - Li, Chengmin
AU  - Li C
AD  - Department of Infectious Disease, The Chongqing Fuling Center Hospital, Chongqing 
      400016, China.
FAU - Shi, Guo
AU  - Shi G
AD  - Department of General Surgery, The First Affiliated Hospital of Chongqing Medical 
      University, Chongqing 400016, China.
FAU - Xu, Xiaolei
AU  - Xu X
AD  - Department of Infectious Disease, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, China.
FAU - Luo, Xue
AU  - Luo X
AD  - Department of Infectious Disease, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, China.
FAU - Zhang, Yuanling
AU  - Zhang Y
AD  - Department of Infectious Disease, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, China.
FAU - Fu, Jingjie
AU  - Fu J
AD  - Department of Infectious Disease, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, China.
FAU - Zeng, Aizhong
AU  - Zeng A
AUID- ORCID: 0000-0002-1397-1581
AD  - Department of Infectious Disease, The First Affiliated Hospital of Chongqing 
      Medical University, Chongqing 400016, China.
FAU - Chen, Limin
AU  - Chen L
AUID- ORCID: 0000-0002-7370-6499
AD  - Toronto General Research Institute, University of Toronto, Toronto, ON, Canada.
LA  - eng
PT  - Journal Article
DEP - 20180715
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cancer Vaccines)
RN  - EC 1.- (Mixed Function Oxygenases)
RN  - EC 1.14.11.- (aspartic acid 2-oxoglutarate-dependent dioxygenase)
SB  - IM
MH  - Adenoviridae
MH  - Animals
MH  - Antigens, Neoplasm/immunology
MH  - Cancer Vaccines/genetics/*immunology
MH  - *Carcinoma, Hepatocellular
MH  - Dendritic Cells/*immunology
MH  - Genetic Vectors
MH  - Humans
MH  - Immunotherapy/*methods
MH  - *Liver Neoplasms
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mixed Function Oxygenases/*genetics
PMC - PMC6079451
EDAT- 2018/08/18 06:00
MHDA- 2018/11/06 06:00
PMCR- 2018/07/15
CRDT- 2018/08/18 06:00
PHST- 2018/01/25 00:00 [received]
PHST- 2018/05/30 00:00 [accepted]
PHST- 2018/08/18 06:00 [entrez]
PHST- 2018/08/18 06:00 [pubmed]
PHST- 2018/11/06 06:00 [medline]
PHST- 2018/07/15 00:00 [pmc-release]
AID - 10.1155/2018/9832467 [doi]
PST - epublish
SO  - J Immunol Res. 2018 Jul 15;2018:9832467. doi: 10.1155/2018/9832467. eCollection 
      2018.