PMID- 3011847
OWN - NLM
STAT- MEDLINE
DCOM- 19860627
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 23
IP  - 5
DP  - 1986 May
TI  - The role of beta-lactamase in staphylococcal resistance to 
      penicillinase-resistant penicillins and cephalosporins.
PG  - 832-9
AB  - We showed that most Staphylococcus aureus strains that have borderline or 
      intermediate susceptibility to the penicillinase-resistant penicillins (PRPs) 
      react this way because of the activity of their beta-lactamase on these 
      antimicrobial agents. These strains produced large amounts of staphylococcal 
      beta-lactamase that rapidly hydrolyzed penicillin and partially hydrolyzed the 
      PRPs. Susceptibility to hydrolysis was penicillin greater than oxacillin greater 
      than cephalothin greater than methicillin. The borderline results and the 
      hydrolysis could be prevented by the beta-lactamase inhibitors clavulanic acid 
      and sulbactam. For intrinsically methicillin-resistant (heteroresistant) S. 
      aureus, the inhibitors reduced the penicillin MICs, but the strains remained 
      resistant to all the beta-lactam antimicrobial agents, including penicillin. We 
      conclude that the borderline in vitro susceptibility or resistance to PRPs in 
      most of these S. aureus strains is mediated by beta-lactamase and they are not 
      heteroresistant or intrinsically resistant. We do not know whether this in vitro 
      resistance is expressed clinically.
FAU - McDougal, L K
AU  - McDougal LK
FAU - Thornsberry, C
AU  - Thornsberry C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Cephalosporins)
RN  - 0 (Clavulanic Acids)
RN  - 0 (Penicillins)
RN  - 0 (beta-Lactamase Inhibitors)
RN  - 23521W1S24 (Clavulanic Acid)
RN  - 87-53-6 (Penicillanic Acid)
RN  - EC 3.5.2.- (Penicillinase)
RN  - EC 3.5.2.6 (beta-Lactamases)
RN  - Q91FH1328A (Methicillin)
RN  - R72LW146E6 (Cephalothin)
RN  - S4TF6I2330 (Sulbactam)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
MH  - Cephalosporins/*pharmacology
MH  - Cephalothin/pharmacology
MH  - Clavulanic Acid
MH  - Clavulanic Acids/pharmacology
MH  - Methicillin/pharmacology
MH  - Microbial Sensitivity Tests
MH  - Oxacillin/pharmacology
MH  - Penicillanic Acid/pharmacology
MH  - *Penicillin Resistance
MH  - Penicillinase/*metabolism
MH  - Penicillins/pharmacology
MH  - Staphylococcus aureus/*drug effects/enzymology
MH  - Sulbactam
MH  - beta-Lactamase Inhibitors
MH  - beta-Lactamases/*metabolism
PMC - PMC268732
EDAT- 1986/05/01 00:00
MHDA- 1986/05/01 00:01
PMCR- 1986/05/01
CRDT- 1986/05/01 00:00
PHST- 1986/05/01 00:00 [pubmed]
PHST- 1986/05/01 00:01 [medline]
PHST- 1986/05/01 00:00 [entrez]
PHST- 1986/05/01 00:00 [pmc-release]
AID - 10.1128/jcm.23.5.832-839.1986 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1986 May;23(5):832-9. doi: 10.1128/jcm.23.5.832-839.1986.