PMID- 30118750 OWN - NLM STAT- MEDLINE DCOM- 20190730 LR - 20190730 IS - 1873-2747 (Electronic) IS - 0361-9230 (Linking) VI - 142 DP - 2018 Sep TI - Osthole alleviated diabetic neuropathic pain mediated by the P2X(4) receptor in dorsal root ganglia. PG - 289-296 LID - S0361-9230(18)30257-0 [pii] LID - 10.1016/j.brainresbull.2018.08.008 [doi] AB - Diabetes mellitus (DM) is considered the primary cause of neuropathic pain. Osthole (7-methoxy-8[3-methylpent 2-enyl]coumarin) is a component extracted from Cnidium monnieri (L.) cusson plant seeds and has anti-inflammatory and anti-oxidative properties. The aim of the present study was to investigate the effects of osthole on diabetic neuropathic pain (DNP) involving the P2X(4) receptor on satellite glial cells (SGCs) in the dorsal root ganglia (DRG) of type 2 diabetic rats. These data showed that the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) in DM rats were lower than those in control rats. MWT and TWL in DM rats treated with osthole were higher compared with those in untreated DM rats. The expression levels of P2X(4) mRNA and protein in the DRG of DM rats were higher compared with those in the control rats, while those in DM rats treated with osthole were significantly lower compared with those in the untreated DM rats. Osthole treatment decreased the co-expression levels of P2X(4) and glial fibrillary acidic protein (GFAP) and reduced the up-regulated expression of interleukin-1 beta (IL-1beta), tumour necrosis factor-alpha (TNF-alpha), brain-derived neurotrophic factor (BDNF) and phosphorylated-p38MAPK and enhanced the down-regulation of IL-10 in DM rats. Thus, osthole treatment may act on the P2X(4) receptor to alleviate the mechanical and thermal hyperalgesia in DM rats. CI - Copyright (c) 2018 Elsevier Inc. All rights reserved. FAU - Yuan, Huilong AU - Yuan H AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Ouyang, Shuai AU - Ouyang S AD - Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Yang, Runan AU - Yang R AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Li, Shunhua AU - Li S AD - Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Gong, Yingxin AU - Gong Y AD - Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Zou, Lifang AU - Zou L AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Jia, Tianyu AU - Jia T AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Zhao, Shanhong AU - Zhao S AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Wu, Bing AU - Wu B AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Yi, Zhihua AU - Yi Z AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Liu, Hui AU - Liu H AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Shi, Liran AU - Shi L AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Li, Lin AU - Li L AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Gao, Yun AU - Gao Y AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Li, Guilin AU - Li G AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Xu, Hong AU - Xu H AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Liu, Shuangmei AU - Liu S AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Zhang, Chunping AU - Zhang C AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. FAU - Liang, Shangdong AU - Liang S AD - Department of Physiology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China; Jiangxi Provincial Key Laboratory of Autonomic Nervous Function and Disease, Nanchang, Jiangxi 330006, People's Republic of China; Department of Cell Biology, Medical School of Nanchang University, Nanchang, Jiangxi 330006, People's Republic of China. Electronic address: liangsd@hotmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180814 PL - United States TA - Brain Res Bull JT - Brain research bulletin JID - 7605818 RN - 0 (Analgesics) RN - 0 (Coumarins) RN - 0 (Hypoglycemic Agents) RN - 0 (Purinergic P2X Receptor Antagonists) RN - 0 (Receptors, Purinergic P2X4) RN - XH1TI1759C (osthol) MH - Analgesics/*pharmacology MH - Animals MH - Coumarins/*pharmacology MH - Diabetes Mellitus, Experimental/drug therapy/metabolism/pathology MH - Diabetic Neuropathies/*drug therapy/metabolism/pathology MH - Ganglia, Spinal/*drug effects/metabolism/pathology MH - Hyperalgesia/drug therapy/metabolism/pathology MH - Hypoglycemic Agents/pharmacology MH - Male MH - Neuralgia/*drug therapy/metabolism/pathology MH - Pain Threshold/drug effects/physiology MH - Purinergic P2X Receptor Antagonists/pharmacology MH - Random Allocation MH - Rats, Sprague-Dawley MH - Receptors, Purinergic P2X4/*metabolism MH - Up-Regulation/drug effects OTO - NOTNLM OT - Diabetic neuropathic pain OT - Dorsal root ganglia OT - Osthole OT - P2X(4)receptor OT - Satellite glial cells EDAT- 2018/08/18 06:00 MHDA- 2019/07/31 06:00 CRDT- 2018/08/18 06:00 PHST- 2018/04/04 00:00 [received] PHST- 2018/08/10 00:00 [revised] PHST- 2018/08/11 00:00 [accepted] PHST- 2018/08/18 06:00 [pubmed] PHST- 2019/07/31 06:00 [medline] PHST- 2018/08/18 06:00 [entrez] AID - S0361-9230(18)30257-0 [pii] AID - 10.1016/j.brainresbull.2018.08.008 [doi] PST - ppublish SO - Brain Res Bull. 2018 Sep;142:289-296. doi: 10.1016/j.brainresbull.2018.08.008. Epub 2018 Aug 14.