PMID- 30119168
OWN - NLM
STAT- MEDLINE
DCOM- 20181126
LR  - 20181126
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 106
DP  - 2018 Oct
TI  - Aquilariae Lignum extract attenuates glutamate-induced neuroexcitotoxicity in 
      HT22 hippocampal cells.
PG  - 1031-1038
LID - S0753-3322(18)32203-0 [pii]
LID - 10.1016/j.biopha.2018.07.032 [doi]
AB  - An imbalance between excitatory and inhibitory neurotransmitters is known to 
      induce neuronal excitotoxicity which is a major cause of neurodegenerative 
      disorders. Excessive glutamate concentration leads to the neuronal death by 
      increasing oxidative stress and affecting the apoptotic signaling pathway. We 
      investigated the anti-excitotoxic effects and associated working mechanisms of 
      30% ethanol extract of Aquilariae Lignum (ALE) against hippocampal neuronal death 
      by glutamate. HT22 cells were treated with glutamate (20 mM) for 24 h following 
      pretreatment with ALE (5, 10, 25 mug/mL). Cell viability, biochemical analysis, 
      flow chemistry, and Western blotting assays were performed. Glutamate treatment 
      substantially increased the intracellular level of reactive oxygen species (ROS) 
      and Ca(2+) influx into the cell, which were followed by apoptosis. ALE 
      pretreatment, however, significantly attenuated these excitotoxicity-related 
      features according to the results of Annexin V analysis and the lactate 
      dehydrogenase assay, in which the calpain pathway (in a caspase 3-independent 
      manner) may be involved. ALE pretreatment also significantly attenuated the 
      glutamate-induced activation of both inflammation-associated molecules 
      (extracellular signal-regulated kinase, c-Jun N-terminal kinases and p38) and 
      death-related molecules (p53, apoptosis-inducing factor). The inactivation of 
      brain-derived neurotrophic factor (BDNF) was restored by ALE pretreatment. Our 
      results verified that A. Lignum has potential neuroprotective effects on 
      glutamate-induced excitotoxicity in hippocampal neuron cells, and its underlying 
      mechanism may involve the regulation of ROS-mediated cell death pathways.
CI  - Copyright (c) 2018 Elsevier Masson SAS. All rights reserved.
FAU - Lee, Jin-Seok
AU  - Lee JS
AD  - Liver and Immunology Research Center, Oriental Medical Collage of Daejeon 
      University, 22-5 Daehung-dong, Jung-gu, Daejeon, 301-724, Republic of Korea.
FAU - Kim, Won-Yong
AU  - Kim WY
AD  - Liver and Immunology Research Center, Oriental Medical Collage of Daejeon 
      University, 22-5 Daehung-dong, Jung-gu, Daejeon, 301-724, Republic of Korea.
FAU - Jeon, Yoo-Jin
AU  - Jeon YJ
AD  - Liver and Immunology Research Center, Oriental Medical Collage of Daejeon 
      University, 22-5 Daehung-dong, Jung-gu, Daejeon, 301-724, Republic of Korea.
FAU - Lee, Sam-Keun
AU  - Lee SK
AD  - Department of Applied Chemistry, Oriental Medicine Collage of Daejeon University, 
      62, Daehak-ro, Dong-gu, Daejeon, 300-716, Republic of Korea.
FAU - Son, Chang-Gue
AU  - Son CG
AD  - Liver and Immunology Research Center, Oriental Medical Collage of Daejeon 
      University, 22-5 Daehung-dong, Jung-gu, Daejeon, 301-724, Republic of Korea. 
      Electronic address: ckson@dju.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20180717
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Excitatory Amino Acid Antagonists)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - EC 3.4.22.- (Calpain)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Calcium Signaling/drug effects
MH  - Calpain/metabolism
MH  - Cell Line
MH  - Dose-Response Relationship, Drug
MH  - Excitatory Amino Acid Agonists/*toxicity
MH  - Excitatory Amino Acid Antagonists/isolation & purification/*pharmacology
MH  - Glutamic Acid/*toxicity
MH  - Hippocampus/*drug effects/metabolism/pathology
MH  - Mice
MH  - Neurons/*drug effects/metabolism/pathology
MH  - Neuroprotective Agents/isolation & purification/*pharmacology
MH  - Phytotherapy
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - Plants, Medicinal
MH  - Reactive Oxygen Species/metabolism
MH  - *Thymelaeaceae/chemistry
MH  - Time Factors
OTO - NOTNLM
OT  - Apoptosis
OT  - Aquilariae Lignum
OT  - Calcium overload
OT  - Calpain-dependent
OT  - Excitotoxicity
EDAT- 2018/08/19 06:00
MHDA- 2018/11/27 06:00
CRDT- 2018/08/19 06:00
PHST- 2018/04/02 00:00 [received]
PHST- 2018/07/05 00:00 [revised]
PHST- 2018/07/06 00:00 [accepted]
PHST- 2018/08/19 06:00 [pubmed]
PHST- 2018/11/27 06:00 [medline]
PHST- 2018/08/19 06:00 [entrez]
AID - S0753-3322(18)32203-0 [pii]
AID - 10.1016/j.biopha.2018.07.032 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2018 Oct;106:1031-1038. doi: 10.1016/j.biopha.2018.07.032. 
      Epub 2018 Jul 17.