PMID- 30119179
OWN - NLM
STAT- MEDLINE
DCOM- 20181126
LR  - 20181202
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 106
DP  - 2018 Oct
TI  - Hydrogen alleviates cellular senescence via regulation of ROS/p53/p21 pathway in 
      bone marrow-derived mesenchymal stem cells in vivo.
PG  - 1126-1134
LID - S0753-3322(18)32840-3 [pii]
LID - 10.1016/j.biopha.2018.07.020 [doi]
AB  - Senescence has become a hot point issue in recent decades and requires urgent 
      attention. As a novel and effective antioxidant, hydrogen has been proved to 
      alleviate cellular senescence in endothelial cells in vitro. However, the effects 
      and mechanisms of hydrogen on senescence in vivo are still unclear. In the 
      present study, 12-month-old Sprague Dawley (SD) rats were intraperitoneal 
      administration of hydrogen-rich saline (HRS, 10 ml/kg). Subsequently, bone 
      marrow-derived stem cells (BMSCs) were harvested for the detection of hydrogen 
      antisenescence effects and mechanisms. The results showed that the number of 
      senescence-associated beta-galactosidase (SA-beta-Gal) positive cells was reduced in 
      BMSCs from rats treated with HRS. BMSCs in rats treated with HRS possessed a 
      better proliferation ability, showed more effectively tri-lineage differentiation 
      potential, and had less percentage of cells in G1 cell cycle arrest than the 
      control cells. Additionally, HRS administration inhibited the production of 
      intracellular reactive oxygen species (ROS) and decreased the expression of 
      senescence-related proteins p53 and p21. Our results revealed that hydrogen could 
      alleviate cellular senescence in vivo. And the underlying mechanism of 
      antisenescence effects of hydrogen in BMSCs was via the ROS/p53/p21 signaling 
      pathway. Thus, hydrogen could be a new and convenient strategy for alleviating 
      senescence and for therapy of age-related diseases.
CI  - Copyright (c) 2018 Elsevier Masson SAS. All rights reserved.
FAU - Zhang, Wenbo
AU  - Zhang W
AD  - First Clinical College, Chongqing Medical University, #1 Yi-Xue-Yuan Road, 
      Chongqing, 400016, China.
FAU - Huang, Chao
AU  - Huang C
AD  - Department of Anatomy, Institute of Biomedical Engineering, Second Military 
      Medical University, #800 Xiangyin Road, Shanghai, 200433, China.
FAU - Sun, Aijun
AU  - Sun A
AD  - Department of Anatomy, Institute of Biomedical Engineering, Second Military 
      Medical University, #800 Xiangyin Road, Shanghai, 200433, China.
FAU - Qiao, Liang
AU  - Qiao L
AD  - Department of Anatomy, Institute of Biomedical Engineering, Second Military 
      Medical University, #800 Xiangyin Road, Shanghai, 200433, China.
FAU - Zhang, Xi
AU  - Zhang X
AD  - Department of Anatomy, Institute of Biomedical Engineering, Second Military 
      Medical University, #800 Xiangyin Road, Shanghai, 200433, China.
FAU - Huang, Junlong
AU  - Huang J
AD  - Department of Navy Aeromedicine, Second Military Medical University, #800 
      Xiangyin Road, Shanghai, 200433, China.
FAU - Sun, Xuejun
AU  - Sun X
AD  - Department of Navy Aeromedicine, Second Military Medical University, #800 
      Xiangyin Road, Shanghai, 200433, China.
FAU - Yang, Xiangqun
AU  - Yang X
AD  - Department of Anatomy, Institute of Biomedical Engineering, Second Military 
      Medical University, #800 Xiangyin Road, Shanghai, 200433, China. Electronic 
      address: yangxq_sh@126.com.
FAU - Sun, Shanquan
AU  - Sun S
AD  - Department of Anatomy, Chongqing Medical University, #1 Yi-Xue-Yuan Road, 
      Chongqing, 400016, China. Electronic address: sunsq2151@sohu.com.
LA  - eng
PT  - Journal Article
DEP - 20180717
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Antioxidants)
RN  - 0 (Cdkn1a protein, rat)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Bone Marrow Cells/*drug effects/enzymology
MH  - Cell Differentiation/drug effects
MH  - Cell Lineage
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Cellular Senescence/*drug effects
MH  - Cyclin-Dependent Kinase Inhibitor p21/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - G1 Phase Cell Cycle Checkpoints/drug effects
MH  - Hydrogen/*pharmacology
MH  - Male
MH  - Mesenchymal Stem Cells/*drug effects/enzymology
MH  - Oxidative Stress/drug effects
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/*metabolism
MH  - Signal Transduction/drug effects
MH  - Time Factors
MH  - Tumor Suppressor Protein p53/*metabolism
OTO - NOTNLM
OT  - Antisenescence
OT  - Bone marrow-derived mesenchymal stem cells
OT  - Hydrogen
OT  - ROS/p53/p21
EDAT- 2018/08/19 06:00
MHDA- 2018/11/27 06:00
CRDT- 2018/08/19 06:00
PHST- 2018/04/29 00:00 [received]
PHST- 2018/07/02 00:00 [revised]
PHST- 2018/07/03 00:00 [accepted]
PHST- 2018/08/19 06:00 [pubmed]
PHST- 2018/11/27 06:00 [medline]
PHST- 2018/08/19 06:00 [entrez]
AID - S0753-3322(18)32840-3 [pii]
AID - 10.1016/j.biopha.2018.07.020 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2018 Oct;106:1126-1134. doi: 10.1016/j.biopha.2018.07.020. 
      Epub 2018 Jul 17.