PMID- 30120810
OWN - NLM
STAT- MEDLINE
DCOM- 20190726
LR  - 20240402
IS  - 1600-0854 (Electronic)
IS  - 1398-9219 (Print)
IS  - 1398-9219 (Linking)
VI  - 19
IP  - 11
DP  - 2018 Nov
TI  - T-complex protein 1-ring complex enhances retrograde axonal transport by 
      modulating tau phosphorylation.
PG  - 840-853
LID - 10.1111/tra.12610 [doi]
AB  - The cytosolic chaperonin T-complex protein (TCP) 1-ring complex (TRiC) has been 
      shown to exert neuroprotective effects on axonal transport through clearance of 
      mutant Huntingtin (mHTT) in Huntington's disease. However, it is presently 
      unknown if TRiC also has any effect on axonal transport in wild-type neurons. 
      Here, we examined how TRiC impacted the retrograde axonal transport of 
      brain-derived neurotrophic factor (BDNF). We found that expression of a single 
      TRiC subunit significantly enhanced axonal transport of BDNF, leading to an 
      increase in instantaneous velocity with a concomitant decrease in pauses for 
      retrograde BDNF transport. The transport enhancing effect by TRiC was dependent 
      on endogenous tau expression because no effect was seen in neurons from tau 
      knockout mice. We showed that TRiC regulated the level of cyclin-dependent kinase 
      5 (CDK5)/p35 positively, contributing to TRiC-mediated tau phosphorylation 
      (ptau). Expression of a single TRiC subunit increased the level of ptau while 
      downregulation of the TRiC complex decreased ptau. We further demonstrated that 
      TRiC-mediated increase in ptau induced detachment of tau from microtubules. Our 
      study has thus revealed that TRiC-mediated increase in tau phosphorylation 
      impacts retrograde axonal transport.
CI  - (c) 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Chen, Xu-Qiao
AU  - Chen XQ
AD  - Department of Neurosciences, University of California San Diego, La Jolla, 
      California.
FAU - Fang, Fang
AU  - Fang F
AD  - Department of Neurosciences, University of California San Diego, La Jolla, 
      California.
FAU - Florio, Jazmin B
AU  - Florio JB
AD  - Department of Neurosciences, University of California San Diego, La Jolla, 
      California.
FAU - Rockenstein, Edward
AU  - Rockenstein E
AD  - Department of Neurosciences, University of California San Diego, La Jolla, 
      California.
FAU - Masliah, Eliezer
AU  - Masliah E
AD  - Department of Neurosciences, University of California San Diego, La Jolla, 
      California.
FAU - Mobley, William C
AU  - Mobley WC
AD  - Department of Neurosciences, University of California San Diego, La Jolla, 
      California.
FAU - Rissman, Robert A
AU  - Rissman RA
AD  - Department of Neurosciences, University of California San Diego, La Jolla, 
      California.
AD  - Veterans Affairs San Diego Healthcare System, San Diego, California.
FAU - Wu, Chengbiao
AU  - Wu C
AUID- ORCID: 0000-0002-3660-088X
AD  - Department of Neurosciences, University of California San Diego, La Jolla, 
      California.
AD  - Veterans Affairs San Diego Healthcare System, San Diego, California.
LA  - eng
GR  - R21 AG051839/AG/NIA NIH HHS/United States
GR  - P50 AG005131/AG/NIA NIH HHS/United States
GR  - I01 BX003040/BX/BLRD VA/United States
GR  - R01 AG051848/AG/NIA NIH HHS/United States
GR  - R01 AG018440/AG/NIA NIH HHS/United States
GR  - R37 AG018440/AG/NIA NIH HHS/United States
GR  - P01 NS092525/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20180912
PL  - England
TA  - Traffic
JT  - Traffic (Copenhagen, Denmark)
JID - 100939340
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (tau Proteins)
RN  - EC 2.7.11.1 (Cyclin-Dependent Kinase 5)
RN  - EC 3.6.1.- (Chaperonin Containing TCP-1)
MH  - Animals
MH  - *Axonal Transport
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - CHO Cells
MH  - Cells, Cultured
MH  - Chaperonin Containing TCP-1/genetics/*metabolism
MH  - Cricetinae
MH  - Cricetulus
MH  - Cyclin-Dependent Kinase 5/metabolism
MH  - HEK293 Cells
MH  - Humans
MH  - Microtubules/metabolism
MH  - Phosphorylation
MH  - Rats
MH  - tau Proteins/*metabolism
PMC - PMC6191364
MID - NIHMS985975
OTO - NOTNLM
OT  - TRiC/CCT chaperonin
OT  - axonal transport
OT  - hyperphosphorylation
OT  - tau
COIS- Conflict of interest The authors declare no conflict of interest.
EDAT- 2018/08/19 06:00
MHDA- 2019/07/28 06:00
PMCR- 2019/11/01
CRDT- 2018/08/19 06:00
PHST- 2018/02/07 00:00 [received]
PHST- 2018/08/09 00:00 [revised]
PHST- 2018/08/12 00:00 [accepted]
PHST- 2018/08/19 06:00 [pubmed]
PHST- 2019/07/28 06:00 [medline]
PHST- 2018/08/19 06:00 [entrez]
PHST- 2019/11/01 00:00 [pmc-release]
AID - 10.1111/tra.12610 [doi]
PST - ppublish
SO  - Traffic. 2018 Nov;19(11):840-853. doi: 10.1111/tra.12610. Epub 2018 Sep 12.