PMID- 30121666
OWN - NLM
STAT- MEDLINE
DCOM- 20180910
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 48
IP  - 6
DP  - 2018
TI  - Mir-382 Promotes Differentiation of Rat Liver Progenitor Cell WB-F344 by 
      Targeting Ezh2.
PG  - 2389-2398
LID - 10.1159/000492654 [doi]
AB  - BACKGROUND/AIMS: Liver progenitor cells (LPCs) were considered as a promising 
      hepatocyte source of cell therapy for liver disease due to their self-renewal and 
      differentiation capacities, while little is known about the mechanism of LPC 
      differentiate into hepatocytes. This study aims to explore the effect of miR-382, 
      a member of Dlk1-Dio3 microRNA cluster, during hepatic differentiation from LPCs. 
      METHODS: In this study, we used rat liver progenitor cell WB-F344 as LPC cell 
      model and HGF as inducer to simulate the process of LPCs hepatic differentiation, 
      then microRNAs were quantified by qPCR. Next, WB-F344 cell was transfected with 
      miR-382 mimics, then hepatocyte cell trait was characterized by multiple 
      experiments, including that periodic acid schiff staining and cellular uptake and 
      excretion of indocyanine green to evaluate the hepatocellular function, qPCR and 
      Western Blotting analysis to detect the hepatocyte-specific markers (ALB, Ttr, 
      Apo E and AFP) and transmission electron microscopy to observe the hepatocellular 
      morphology. Moreover, Luciferase reporter assay was used to determine whether 
      Ezh2 is the direct target of miR-382. RESULTS: We found that miR-382 increased 
      gradually and was inversely correlated with the potential target, Ezh2, during 
      WB-F344 hepatic differentiation. In addition, functional studies indicated that 
      miR-382 increased the level of hepatocyte-specific genes. CONCLUSIONS: This study 
      demonstrates that miR-382 may be a novel regulator of LPCs differentiation by 
      targeting Ezh2.
CI  - (c) 2018 The Author(s). Published by S. Karger AG, Basel.
FAU - Zheng, Yongxia
AU  - Zheng Y
AD  - Department of Forensic Medicine, Shantou University Medical College, Shantou, 
      China.
AD  - Medical College, Jiaxing University, Jiaxing, China.
FAU - Zhou, Jiansheng
AU  - Zhou J
AD  - The 92th Hospital of PLA, Nanping, China.
FAU - Li, Xuebo
AU  - Li X
AD  - Department of Forensic Medicine, Shantou University Medical College, Shantou, 
      China.
AD  - Key Laboratory of Evidence Identification in Universities of Shandong Province, 
      Shandong University of Political Science and Law, Jinan, China.
FAU - Xu, Guangtao
AU  - Xu G
AD  - Medical College, Jiaxing University, Jiaxing, China.
FAU - Jin, Mingliang
AU  - Jin M
AD  - The 92th Hospital of PLA, Nanping, China.
FAU - Shen, Ruilin
AU  - Shen R
AD  - Department of Biochemistry and Diabetes Institute, Jiaxing Hospital of 
      Traditional Chinese Medicine, Jiaxing University, Jiaxing, China.
FAU - Su, Ruibing
AU  - Su R
AD  - Department of Forensic Medicine, Shantou University Medical College, Shantou, 
      China.
FAU - Zhan, Shuyu
AU  - Zhan S
AD  - Medical College, Jiaxing University, Jiaxing, China.
FAU - Ding, Baoyue
AU  - Ding B
AD  - Medical College, Jiaxing University, Jiaxing, China.
FAU - Jia, Mingguang
AU  - Jia M
AD  - Linzi District People's Hospital, Zibo, China.
FAU - Cui, Yuzhong
AU  - Cui Y
AD  - Linzi District People's Hospital, Zibo, China.
FAU - Yu, Xiaojun
AU  - Yu X
AD  - Department of Forensic Medicine, Shantou University Medical College, Shantou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20180817
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Antagomirs)
RN  - 0 (Apolipoproteins E)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Albumin)
RN  - 0 (Serum Albumin)
RN  - 0 (alpha-Fetoproteins)
RN  - 0 (transthyretin receptor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.1.1.43 (EZH2 protein, rat)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Antagomirs/metabolism
MH  - Apolipoproteins E/metabolism
MH  - Base Sequence
MH  - *Cell Differentiation/drug effects
MH  - Cell Line
MH  - Enhancer of Zeste Homolog 2 Protein/antagonists & inhibitors/genetics/*metabolism
MH  - Hepatocyte Growth Factor/pharmacology
MH  - Liver/cytology
MH  - MicroRNAs/antagonists & inhibitors/genetics/*metabolism
MH  - RNA Interference
MH  - RNA, Small Interfering/metabolism
MH  - Rats
MH  - Rats, Inbred F344
MH  - Receptors, Albumin/metabolism
MH  - Sequence Alignment
MH  - Serum Albumin/metabolism
MH  - Stem Cells/cytology/metabolism
MH  - alpha-Fetoproteins/metabolism
OTO - NOTNLM
OT  - Ezh2
OT  - Hepatic differentiation
OT  - MiR-382
EDAT- 2018/08/20 06:00
MHDA- 2018/09/11 06:00
CRDT- 2018/08/20 06:00
PHST- 2017/11/14 00:00 [received]
PHST- 2018/08/06 00:00 [accepted]
PHST- 2018/08/20 06:00 [pubmed]
PHST- 2018/09/11 06:00 [medline]
PHST- 2018/08/20 06:00 [entrez]
AID - 000492654 [pii]
AID - 10.1159/000492654 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2018;48(6):2389-2398. doi: 10.1159/000492654. Epub 2018 Aug 
      17.