PMID- 30121998 OWN - NLM STAT- MEDLINE DCOM- 20181010 LR - 20181023 IS - 1673-0860 (Print) IS - 1673-0860 (Linking) VI - 53 IP - 8 DP - 2018 Aug 7 TI - [The role of NF-kappaB signaling pathway in laryngeal leukoplakia recurrent with laryngeal reflux]. PG - 597-603 LID - 10.3760/cma.j.issn.1673-0860.2018.08.008 [doi] AB - Objective: To study the mechanism of vocal mucosal barrier damage mediated by NF-kappaB and NF-kappaB-regulated signaling pathway via probing the expression of inflammatory factors and essential proteins for node of NF-kappaB signaling pathway. Methods: The patients suffering from vocal leukoplakia accompanied with larygopharyngeal reflux(LPR) were treated with oral administration of proton pump inhibitor(PPI). Mucosal specimens of vocal cord were collected from all patients before PPI treatment. And the mucosal specimens of vocal cord were collected from the patients with suspected recurrence at 8 weeks after PPI treatment. HE staining was used to observe the histopathological changes of the mucosa. ELISA was utilized to detect the levels of inflammatory factors including tumor necrosis factor(TNF)-alpha, interleukin(IL)-1 and IL-6. Western blot was used to detect the expression of p-p65, p-IKK and p-IkappaB. Immunofluorescence method was adopted to detect the entrance of p65 to cell nucleus.Data was analyzed by SPSS 23.0 software. Results: In PPI untreated group, the expressions of IL-1beta, TNF-alpha and IL-6 in the specimens of 8 weeks after operation were not different significantly from those obtained during operation.But in the PPI-treated group, the expressions were down-regulated.The expression of p-p65 in the middle and high grade heterogenous hyperplasia group was higher than that of low level heterogenous hyperplasia group.The difference of p65 and p-p65 expression between 8 weeks after surgery and surgery in PPI-untreated group was statistically insignificant (P>0.05). The difference of p65 expression between PPI-treated group and PPI pre-treatment group was statistically insignificant (P>0.05). The expression of p-p65 in the PPI-treated group was lower than that of the PPI pre-treatment group (P<0.05). The expressions of IL-1beta, TNF-alpha and IL-6 were positively related with that of NF-kappaB-p65. Immun of luorescence method revealed the entrance of p65 to cell nucleus in PPI pre-treatment group, which meant that NF-kappaB was activated. In the PPI-treated group, few activated p65 could be observed in the cell nucleu. Conclusion: The possible mechanism of vocal mucosal barrier damage in vocal leukoplakia accompanied with LPR maybe the vocal mucosal inflammation mediated by NF-kappaB and NF-kappaB-regulated signaling pathway activated with refluxed materials. FAU - Chen, W AU - Chen W AD - Department of Otorhinolaryngology Head and Neck Surgery, PLA Nanjing General Hospital, Nanjing 210002, China. FAU - Zhang, Y AU - Zhang Y AD - Department of Otorhinolaryngology Head and Neck Surgery, PLA Nanjing General Hospital, Nanjing 210002, China. FAU - Cheng, Y AU - Cheng Y AD - Department of Otorhinolaryngology Head and Neck Surgery, PLA Nanjing General Hospital, Nanjing 210002, China. FAU - Wang, Z Y AU - Wang ZY AD - Department of Otorhinolaryngology Head and Neck Surgery, PLA Nanjing General Hospital, Nanjing 210002, China. FAU - Wu, M H AU - Wu MH AD - Department of Otorhinolaryngology Head and Neck Surgery, PLA Nanjing General Hospital, Nanjing 210002, China. FAU - Zhu, M H AU - Zhu MH AD - Department of Otorhinolaryngology Head and Neck Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. FAU - Chen, D H AU - Chen DH AD - Department of Otorhinolaryngology Head and Neck Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. FAU - Zheng, H L AU - Zheng HL AD - Department of Otorhinolaryngology Head and Neck Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China. LA - chi GR - BK20161388/Natural Science Foundation of Jiangsu Province/ GR - QNRC2016917/Project of Invigorating Health Care Through Science, Technology and Education/ GR - 2017M623435/Chinese Postdoctoral Science Foundation/ PT - Journal Article PL - China TA - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi JT - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery JID - 101247574 RN - 0 (Interleukin-1) RN - 0 (Interleukin-1beta) RN - 0 (Interleukin-6) RN - 0 (NF-kappa B) RN - 0 (Proton Pump Inhibitors) RN - 0 (RELA protein, human) RN - 0 (Transcription Factor RelA) RN - 0 (Tumor Necrosis Factor-alpha) SB - IM MH - Down-Regulation MH - Humans MH - Interleukin-1/analysis/metabolism MH - Interleukin-1beta MH - Interleukin-6/analysis/metabolism MH - Laryngeal Diseases/drug therapy/etiology/*metabolism MH - Laryngopharyngeal Reflux/*complications/drug therapy MH - Leukoplakia/drug therapy/etiology/*metabolism MH - NF-kappa B/*physiology MH - Proton Pump Inhibitors/therapeutic use MH - Recurrence MH - Signal Transduction MH - Transcription Factor RelA MH - Tumor Necrosis Factor-alpha/analysis/metabolism MH - Vocal Cords/*metabolism OTO - NOTNLM OT - Barrier OT - Laryngopharyngeal reflux OT - Leukoplakia OT - NF-Kappa B OT - Proton pump inhibitors OT - Vocal cords EDAT- 2018/08/21 06:00 MHDA- 2018/10/12 06:00 CRDT- 2018/08/21 06:00 PHST- 2018/08/21 06:00 [entrez] PHST- 2018/08/21 06:00 [pubmed] PHST- 2018/10/12 06:00 [medline] AID - 10.3760/cma.j.issn.1673-0860.2018.08.008 [doi] PST - ppublish SO - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2018 Aug 7;53(8):597-603. doi: 10.3760/cma.j.issn.1673-0860.2018.08.008.