PMID- 30122107
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1525-6049 (Electronic)
IS  - 0886-022X (Print)
IS  - 0886-022X (Linking)
VI  - 40
IP  - 1
DP  - 2018 Nov
TI  - Low-intensity pulsed ultrasound in combination with SonoVue induces cytotoxicity 
      of human renal glomerular endothelial cells via repression of the ERK1/2 
      signaling pathway.
PG  - 458-465
LID - 10.1080/0886022X.2018.1487868 [doi]
AB  - OBJECTIVES: Low-intensity pulsed ultrasound (LIPUS) and SonoVue have been used 
      widely for diagnosis and therapeutic treatment. The effects of LIPUS and SonoVue 
      on the microvascular system and underlying molecular mechanisms have not been 
      established. METHODS: Cultured human renal glomerular endothelial cells (HRGECs) 
      were treated with 5-min ultrasonic irradiation, 20% SonoVue or the combination of 
      both treatments. Cell proliferation, viablity, and apoptosis were measured by MTT 
      assay, Trypan blue exclusion assay and flow cytometry, respectively. Activation 
      of extracellular regulated protein kinases (ERK) were examined by Western blot. 
      RESULTS: We found that LIPUS and SonoVue alone do not induce cytotoxicity of 
      HRGECs; however, the combination of the two treatments reduces cell proliferation 
      and increases cell death. In addition, the combination of LIPUS and SonoVue 
      suppressed the activation of ERK 1/2 in HRGRCs. With pretreatment of the 
      inhibitor of ERK1/2 signaling, PD98059, LIPUS, and SonoVue does not induce 
      additional cell death and inhibition of proliferation. CONCLUSIONS: LIPUS 
      combined with SonoVue induces cytotoxicity of HRGECs via repression of the ERK1/2 
      signaling pathway.
FAU - Liu, Xiu
AU  - Liu X
AD  - a Shandong Provincial Qianfoshan Hospital, Shandong University , Jinan , China.
AD  - b Department of Cardiography , Yantai Affiliated Hospital of Binzhou Medical 
      University , Yantai , China.
FAU - Wang, Bei
AU  - Wang B
AD  - c Department of Ultrasonography , Shandong Provincial Qianfoshan Hospital, 
      Shandong University , Jinan , China.
FAU - Ding, Hongyu
AU  - Ding H
AD  - c Department of Ultrasonography , Shandong Provincial Qianfoshan Hospital, 
      Shandong University , Jinan , China.
FAU - Shi, Hao
AU  - Shi H
AD  - d Department of Radiology, Shandong Provincial Qianfoshan Hospital , Shandong 
      University , Jinan , China.
FAU - Liu, Ju
AU  - Liu J
AUID- ORCID: 0000-0001-9932-2613
AD  - e Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong 
      University , Jinan , China.
FAU - Sun, Hongjun
AU  - Sun H
AD  - e Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong 
      University , Jinan , China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Ren Fail
JT  - Renal failure
JID - 8701128
RN  - 0 (Flavonoids)
RN  - 0 (Phospholipids)
RN  - 0 (contrast agent BR1)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
RN  - WS7LR3I1D6 (Sulfur Hexafluoride)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Endothelial Cells/drug effects/*metabolism
MH  - Flavonoids/*pharmacology
MH  - Humans
MH  - MAP Kinase Signaling System/*drug effects
MH  - Phospholipids/*adverse effects
MH  - Sulfur Hexafluoride/*adverse effects
MH  - Ultrasonic Waves/*adverse effects
PMC - PMC6104615
OTO - NOTNLM
OT  - ERK1/2 signaling pathway
OT  - Low intensity pulsed ultrasound
OT  - SonoVue
OT  - cytotoxicity
OT  - human renal glomerular endothelial cells
EDAT- 2018/08/21 06:00
MHDA- 2018/12/12 06:00
PMCR- 2018/08/20
CRDT- 2018/08/21 06:00
PHST- 2018/08/21 06:00 [entrez]
PHST- 2018/08/21 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/08/20 00:00 [pmc-release]
AID - 1487868 [pii]
AID - 10.1080/0886022X.2018.1487868 [doi]
PST - ppublish
SO  - Ren Fail. 2018 Nov;40(1):458-465. doi: 10.1080/0886022X.2018.1487868.