PMID- 3012212
OWN - NLM
STAT- MEDLINE
DCOM- 19860722
LR  - 20190824
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 10
IP  - 6
DP  - 1986
TI  - Analysis of anti-HTLV-I antibody by strip radioimmunoassay--comparison with 
      indirect immunofluorescence assay, enzyme-linked immunosorbent assay and membrane 
      immunofluorescence assay.
PG  - 605-10
AB  - Antibodies against human T-cell lymphotropic virus type I (HTLV-I) in the sera 
      from 60 patients with adult T-cell leukemia and 21 carriers who were suspected of 
      having HTLV-I infection were investigated by indirect immunofluorescence assay 
      (IFA), enzyme-linked immunosorbent assay (ELISA), membrane immunofluorescence 
      assay (MIA) and strip radio immunoassay based on the western blotting technique 
      (SRIA). The sera of 2 of the carriers who were seropositive in IFA and ELISA were 
      negative in MIA and did not react with virus-specific proteins by SRIA. Two sera 
      were negative in IFA and ELISA. These sera were positive in MIA and reacted with 
      only the envelope-related glycoprotein (gp46) and not with gag-related proteins 
      (p28, p24, p19) by SRIA. These findings suggest that the main antigens defined by 
      IFA and ELISA are gag-related proteins and some sera which do not contain 
      anti-HTLV-I antibodies give false-positive results because of the reaction to 
      unknown cellular components. Also some sera may have antibodies against only 
      envelope glycoproteins, and these sera may give false-negative results in IFA and 
      ELISA.
FAU - Chosa, T
AU  - Chosa T
FAU - Hattori, T
AU  - Hattori T
FAU - Matsuoka, M
AU  - Matsuoka M
FAU - Yamaguchi, K
AU  - Yamaguchi K
FAU - Yamamoto, S
AU  - Yamamoto S
FAU - Takatsuki, K
AU  - Takatsuki K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Antibodies, Viral/*analysis
MH  - Antibody Specificity
MH  - Antigens, Viral/immunology
MH  - Cell Membrane/immunology
MH  - Deltaretrovirus/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Fluorescent Antibody Technique
MH  - Immunosorbent Techniques
MH  - Leukemia/immunology/microbiology
MH  - Molecular Weight
MH  - Radioimmunoassay/methods
EDAT- 1986/01/01 00:00
MHDA- 1986/01/01 00:01
CRDT- 1986/01/01 00:00
PHST- 1986/01/01 00:00 [pubmed]
PHST- 1986/01/01 00:01 [medline]
PHST- 1986/01/01 00:00 [entrez]
AID - 10.1016/0145-2126(86)90262-6 [doi]
PST - ppublish
SO  - Leuk Res. 1986;10(6):605-10. doi: 10.1016/0145-2126(86)90262-6.