PMID- 30122316
OWN - NLM
STAT- MEDLINE
DCOM- 20190114
LR  - 20190114
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 503
IP  - 4
DP  - 2018 Sep 18
TI  - Role of inositol 1,4,5-trisphosphate receptor type 1 in ATP-induced nuclear 
      Ca(2+) signal and hypertrophy in atrial myocytes.
PG  - 2998-3002
LID - S0006-291X(18)31768-6 [pii]
LID - 10.1016/j.bbrc.2018.08.084 [doi]
AB  - Inositol 1,4,5-trisphosphate receptor type 1 (IP(3)R1) is expressed in atrial 
      muscle, but not in ventricle, and they are abundant in the perinucleus. We 
      investigated the role of IP(3)R1 in the regulations of local Ca(2+) signal and 
      cell size in HL-1 atrial myocytes under stimulation by IP(3)-generating chemical 
      messenger, ATP. Assessment of nuclear and cytosolic Ca(2+) signal using confocal 
      Ca(2+) imaging revealed that IP(3) generation by ATP (1 mM) induced monophasic 
      nuclear Ca(2+) increase, followed by cytosolic Ca(2+) oscillation. Genetic 
      knock-down (KD) of IP(3)R1 eliminated the monophasic nuclear Ca(2+) signal and 
      slowed the cytosolic Ca(2+) oscillation upon ATP exposure. Prolonged application 
      of ATP as well as other known hypertrophic agonists (endothelin-1 and 
      phenylephrine) increased cell size in wild-type cells, but not in IP(3)R1 KD 
      cells. Our data indicate that IP(3)R1 mediates sustained elevation in nuclear 
      Ca(2+) level and facilitates cytosolic Ca(2+) oscillation upon external ATP 
      increase, and further suggests possible role of nuclear IP(3)R1 in atrial 
      hypertrophy.
CI  - Copyright (c) 2018 Elsevier Inc. All rights reserved.
FAU - Kim, Joon-Chul
AU  - Kim JC
AD  - Laboratory of Physiology, College of Pharmacy, Chungnam National University, 99 
      Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea.
FAU - Son, Min-Jeong
AU  - Son MJ
AD  - Laboratory of Physiology, College of Pharmacy, Chungnam National University, 99 
      Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea.
FAU - Le, Qui Anh
AU  - Le QA
AD  - Laboratory of Physiology, College of Pharmacy, Chungnam National University, 99 
      Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea.
FAU - Woo, Sun-Hee
AU  - Woo SH
AD  - Laboratory of Physiology, College of Pharmacy, Chungnam National University, 99 
      Daehak-ro, Yuseong-gu, Daejeon, 34134, South Korea. Electronic address: 
      shwoo@cnu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180816
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Inositol 1,4,5-Trisphosphate Receptors)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/*pharmacology
MH  - Animals
MH  - Calcium Signaling/*drug effects
MH  - Cardiomegaly/etiology
MH  - Cell Enlargement/drug effects
MH  - Cell Line
MH  - Cell Nucleus/metabolism
MH  - Heart Atria/pathology
MH  - Hypertrophy/*etiology
MH  - Inositol 1,4,5-Trisphosphate Receptors/*physiology
MH  - Mice
MH  - Myocytes, Cardiac/*pathology
OTO - NOTNLM
OT  - ATP
OT  - Atrial myocytes
OT  - Hypertrophy
OT  - IP(3)R1
OT  - Nuclear Ca(2+)
EDAT- 2018/08/21 06:00
MHDA- 2019/01/15 06:00
CRDT- 2018/08/21 06:00
PHST- 2018/08/03 00:00 [received]
PHST- 2018/08/10 00:00 [accepted]
PHST- 2018/08/21 06:00 [pubmed]
PHST- 2019/01/15 06:00 [medline]
PHST- 2018/08/21 06:00 [entrez]
AID - S0006-291X(18)31768-6 [pii]
AID - 10.1016/j.bbrc.2018.08.084 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Sep 18;503(4):2998-3002. doi: 
      10.1016/j.bbrc.2018.08.084. Epub 2018 Aug 16.