PMID- 30123289
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2018
DP  - 2018
TI  - Alteration in the Cytokine Secretion of Bone Marrow Stromal Cells from Patients 
      with Chronic Myelomonocytic Leukemia Contribute to Impaired Hematopoietic 
      Supportive Activity.
PG  - 5921392
LID - 10.1155/2018/5921392 [doi]
LID - 5921392
AB  - Bone marrow stromal cells (BMSCs) represent an important cellular component of 
      the bone marrow microenvironment, which play an important role in supporting and 
      regulating the proliferation and differentiation of hematopoietic stem/progenitor 
      cells (HSPCs). We have previously reported that the ability of BMSCs derived from 
      CMML patients (CMML-BMSCs) in supporting the expansion of cord blood (CB) CD34(+) 
      cells was significantly reduced compared to BMSCs derived from healthy donors 
      (HD-BMSCs). In addition, CMML-BMSCs led to a skewed differentiation of CB CD34(+) 
      cells favoring myeloid lineage compared with HD-BMSCs. To assess whether the 
      altered cytokine secretion was one of the mechanisms to mediate the impaired 
      hematopoietic supportive activity of CMML-BMSCs, a transwell coculture followed 
      by cytokine array was performed. We showed that noncontacted coculture with 
      CMML-BMSCs preferentially promoted the differentiation of CB CD34(+) cells toward 
      myeloid lineage. The expression levels of multiple cytokines (IL-6, IL-8, and 
      GRO-beta) were markedly reduced in CMML-BMSCs compared with HD-BMSCs. By 
      supplementing IL-6, IL-8, or GRO-beta, the hematopoietic supportive activity of 
      CMML-BMSCs was partially restored. These results suggested that BMSCs may 
      contribute to the pathogenesis of CMML by altering their cytokine secretion, 
      which will shed light on the further investigation to develop novel therapeutic 
      strategies for CMML patients.
FAU - Shi, Hui
AU  - Shi H
AUID- ORCID: 0000-0002-5819-1220
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood 
      Diseases Hospital, Center for Stem Cell Medicine, Chinese Academy of Medical 
      Sciences & Peking Union Medical College, Tianjin, China.
FAU - Wang, Yingshao
AU  - Wang Y
AUID- ORCID: 0000-0002-1381-9985
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood 
      Diseases Hospital, Center for Stem Cell Medicine, Chinese Academy of Medical 
      Sciences & Peking Union Medical College, Tianjin, China.
FAU - Li, Rong
AU  - Li R
AUID- ORCID: 0000-0002-1805-810X
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood 
      Diseases Hospital, Center for Stem Cell Medicine, Chinese Academy of Medical 
      Sciences & Peking Union Medical College, Tianjin, China.
FAU - Xing, Wen
AU  - Xing W
AUID- ORCID: 0000-0002-3380-3374
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood 
      Diseases Hospital, Center for Stem Cell Medicine, Chinese Academy of Medical 
      Sciences & Peking Union Medical College, Tianjin, China.
FAU - Yang, Feng-Chun
AU  - Yang FC
AD  - Department of Biochemistry and Molecular Biology, University of Miami Leonard M. 
      Miller School of Medicine, Miami, FL, USA.
AD  - Sylvester Comprehensive Cancer Center, University of Miami Leonard M. Miller 
      School of Medicine, Miami, FL, USA.
FAU - Bai, Jie
AU  - Bai J
AUID- ORCID: 0000-0002-7493-8347
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood 
      Diseases Hospital, Center for Stem Cell Medicine, Chinese Academy of Medical 
      Sciences & Peking Union Medical College, Tianjin, China.
AD  - Department of Hematology, The Second Hospital of Tianjin Medical University, 
      Tianjin, China.
FAU - Zhou, Yuan
AU  - Zhou Y
AUID- ORCID: 0000-0003-4413-7490
AD  - State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood 
      Diseases Hospital, Center for Stem Cell Medicine, Chinese Academy of Medical 
      Sciences & Peking Union Medical College, Tianjin, China.
LA  - eng
PT  - Journal Article
DEP - 20180710
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC6079359
EDAT- 2018/08/21 06:00
MHDA- 2018/08/21 06:01
PMCR- 2018/07/10
CRDT- 2018/08/21 06:00
PHST- 2018/02/15 00:00 [received]
PHST- 2018/05/03 00:00 [revised]
PHST- 2018/05/15 00:00 [accepted]
PHST- 2018/08/21 06:00 [entrez]
PHST- 2018/08/21 06:00 [pubmed]
PHST- 2018/08/21 06:01 [medline]
PHST- 2018/07/10 00:00 [pmc-release]
AID - 10.1155/2018/5921392 [doi]
PST - epublish
SO  - Stem Cells Int. 2018 Jul 10;2018:5921392. doi: 10.1155/2018/5921392. eCollection 
      2018.