PMID- 30124203
OWN - NLM
STAT- MEDLINE
DCOM- 20181114
LR  - 20181114
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 43
IP  - 7
DP  - 2018 Jul 28
TI  - [SIRT1 deficiency in CD4+T cells induces acute graft-versus-host disease after 
      allogeneic hematopoietic stem cell transplantation].
PG  - 697-703
LID - 10.11817/j.issn.1672-7347.2018.07.001 [doi]
AB  - To study the relationship between acute graft-versus-host disease (aGVHD) and the 
      SIRT1 expression in peripheral blood CD4+T cells from patients after allogeneic 
      hematopoietic stem cell transplantation (allo-HSCT).
 Methods: We collected 40 
      patients who underwent allo-HSCT from human leukocyte antigen (HLA)-identical 
      sibling donors. SIRT1 expression level in CD4+T cells was measured by real-time 
      PCR and Western blot. Acetylation and phosphorylation of STAT3 in CD4+T cells 
      were detected by Western blot. The binding level between SIRT1 and STAT3 in CD4+T 
      cells was analyzed by co-immunoprecipitation and Western blot. Over-expression of 
      SIRT1 in aGVHD CD4+T cells, as well as STAT3 acetylation and phosphorylation were 
      measured by Western blot. The mRNA levels of RORgammat, IL-17A, IL-17F related to 
      Th17 were detected by real-time PCR.
 Results: SIRT1 expression was significantly 
      down-regulated, while STAT3 expression, acetylation and phosphorylation levels 
      were significantly up-regulated in patients with aGVHD compared with patients 
      without aGVHD. The STAT3 acetylation was positively correlated with STAT3 
      phosphorylation (r=0.69, P<0.01). Less SIRT1-STAT3 complexes were found in CD4+T 
      cells from patients with aGVHD compared with patients without aGVHD. After SIRT1 
      over-expression in aGVHD CD4+T cells, the STAT3 acetylation and phosphorylation, 
      and the expression of RORgammat, IL-17A, and IL-17F related to Th17 were 
      significantly down-regulated (P<0.05).
 Conclusion: SIRT1 deficiency in CD4+T 
      cells plays a crucial role in up-regulation of STAT3 acetylation and 
      phosphorylation, the increase of Th17 related gene expression, and induction of 
      aGVHD after allogeneic hematopoietic stem cell transplantation.
FAU - Zhang, Yuanyuan
AU  - Zhang Y
AD  - Department of Hematology, Xiangya Hospital, Central South University, Changsha 
      410008, China.
FAU - Yang, Jing
AU  - Yang J
AD  - Department of Hematology, Xiangya Hospital, Central South University, Changsha 
      410008, China.
FAU - Zhang, Guoping
AU  - Zhang G
AD  - Department of Hematology, Xiangya Hospital, Central South University, Changsha 
      410008, China.
FAU - Peng, Jie
AU  - Peng J
AD  - Department of Hematology, Xiangya Hospital, Central South University, Changsha 
      410008, China.
FAU - Chen, Xu
AU  - Chen X
AD  - Department of Hematology, Xiangya Hospital, Central South University, Changsha 
      410008, China.
FAU - Chen, Fangping
AU  - Chen F
AD  - Department of Hematology, Xiangya Hospital, Central South University, Changsha 
      410008, China.
FAU - Xu, Yajing
AU  - Xu Y
AD  - Department of Hematology, Xiangya Hospital, Central South University, Changsha 
      410008, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (IL17A protein, human)
RN  - 0 (Interleukin-17)
RN  - 0 (Nuclear Receptor Subfamily 1, Group F, Member 3)
RN  - 0 (RORC protein, human)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
RN  - 0 (histocompatibility antigen DLA)
RN  - EC 3.5.1.- (SIRT1 protein, human)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Acute Disease
MH  - CD4-Positive T-Lymphocytes/*metabolism
MH  - Down-Regulation
MH  - Graft vs Host Disease/*etiology/metabolism
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Histocompatibility Antigens Class I
MH  - Humans
MH  - Interleukin-17/metabolism
MH  - Nuclear Receptor Subfamily 1, Group F, Member 3/metabolism
MH  - STAT3 Transcription Factor/*metabolism
MH  - Sirtuin 1/*deficiency/metabolism
MH  - Transplantation, Homologous
MH  - Up-Regulation
EDAT- 2018/08/21 06:00
MHDA- 2018/11/15 06:00
CRDT- 2018/08/21 06:00
PHST- 2018/08/21 06:00 [entrez]
PHST- 2018/08/21 06:00 [pubmed]
PHST- 2018/11/15 06:00 [medline]
AID - 10.11817/j.issn.1672-7347.2018.07.001 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2018 Jul 28;43(7):697-703. doi: 
      10.11817/j.issn.1672-7347.2018.07.001.