PMID- 30126419
OWN - NLM
STAT- MEDLINE
DCOM- 20181211
LR  - 20181211
IS  - 1746-1596 (Electronic)
IS  - 1746-1596 (Linking)
VI  - 13
IP  - 1
DP  - 2018 Aug 21
TI  - MET-overexpressing myxofibrosarcoma frequently exhibit polysomy of chromosome 7 
      but not MET amplification, especially in high-grade cases: clinical and 
      pathological review of 30 myxofibrosarcoma cases.
PG  - 56
LID - 10.1186/s13000-018-0733-9 [doi]
LID - 56
AB  - BACKGROUND: Myxofibrosarcoma (MFS) is one of the most common soft tissue 
      sarcomas. Previous studies have shown that MET protein overexpressed in MFS 
      patients and can serve as a prognostic factor. The reasons for MET protein 
      overexpression include amplification of the MET gene, which is located on 
      chromosome 7q. Triggered by an index case harboring chromosome 7 polysomy rather 
      than MET gene amplification in myxofibrosarcoma, we investigated chromosome 7 
      polysomy in more cases. METHODS: Immunohistochemistry and fluorescence in situ 
      hybridization (FISH) were performed in 30 MFS cases (including 2 epithelioid 
      variant) to detect the expression of MET protein and gene status. RESULTS: MET 
      was overexpressed in 14 cases out of 30, while thirteen cases were in higher 
      FNCLCC grades (Grade 2-3). FISH showed that 11 cases having 3 signals on average 
      of Met and more than 3 signals (Mean: 4.6) of centromere 7q (CEP7q). The MET/CEP7 
      ratio was about 0.65 on average, suggesting that chromosome 7 polysomy, rather 
      than Met gene amplification, leading to the overexpression of MET protein in MFS. 
      MET overexpression and chromosome 7 polysomy are positively correlated with 
      higher Ki-67 index and higher grade and might have a high risk of local 
      recurrence and metastasis. CONCLUSIONS: It might reveals another explain of MET 
      overexpression in myxofibrosarcoma, providing a clue for the therapy of MFS.
FAU - Ma, Shirong
AU  - Ma S
AD  - State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, 
      Fourth Military Medical University, West Road #169, Xi'an, Changle, 710032, 
      China.
FAU - Fan, Linni
AU  - Fan L
AD  - State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, 
      Fourth Military Medical University, West Road #169, Xi'an, Changle, 710032, 
      China.
FAU - Liu, Yixiong
AU  - Liu Y
AD  - State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, 
      Fourth Military Medical University, West Road #169, Xi'an, Changle, 710032, 
      China.
FAU - Wang, Yingmei
AU  - Wang Y
AD  - State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, 
      Fourth Military Medical University, West Road #169, Xi'an, Changle, 710032, 
      China.
FAU - Yu, Kangjie
AU  - Yu K
AD  - Student Team 1, Class 3, Fourth Military Medical University, West Road #169, 
      Xi'an, Changle, 710032, China.
FAU - Wang, Lifeng
AU  - Wang L
AD  - Department of Pathology, Xinhua Hospital Affiliated to Shanghai Jiao Tong 
      University School of Medicine, Kongjiang Road #1665, Shanghai, 200092, China.
FAU - Fang, Na
AU  - Fang N
AD  - Department of Pathology, Hubei Provincial Cancer hospital, Zhuodaoquan South Road 
      #116, Wuhan, 430079, China.
FAU - Liu, Fang
AU  - Liu F
AD  - Department of Pathology, Affiliated Foshan hospital, Sun Yet-sen University, 
      Lingnan North Road#81, Foshan, 528000, China.
FAU - Guo, Shuangping
AU  - Guo S
AD  - State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, 
      Fourth Military Medical University, West Road #169, Xi'an, Changle, 710032, 
      China.
FAU - Wang, Zhe
AU  - Wang Z
AD  - State Key Laboratory of Cancer Biology, Department of Pathology, Xijing Hospital, 
      Fourth Military Medical University, West Road #169, Xi'an, Changle, 710032, 
      China. zhwang@fmmu.edu.cn.
LA  - eng
GR  - 81472402/National Natural Science Foundation of China/
GR  - 81272651/National Natural Science Foundation of China/
GR  - 81570180/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20180821
PL  - England
TA  - Diagn Pathol
JT  - Diagnostic pathology
JID - 101251558
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (MKI67 protein, human)
RN  - EC 2.7.10.1 (MET protein, human)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/analysis/*genetics
MH  - Cell Proliferation
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 7
MH  - Female
MH  - Fibroma/*genetics/*pathology
MH  - *Gene Amplification
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Ki-67 Antigen/analysis
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Phenotype
MH  - Proto-Oncogene Proteins c-met/analysis/*genetics
MH  - Soft Tissue Neoplasms/chemistry/*genetics/*pathology
MH  - Up-Regulation
MH  - Young Adult
PMC - PMC6102798
OTO - NOTNLM
OT  - Chromosome 7 polysomy
OT  - FISH
OT  - MET
OT  - Myxofibrosarcoma
COIS- ETHICS APPROVAL AND CONSENT TO PARTICIPATE: The study procedures were approved by 
      the Ethics Committee of Xijing Hospital (reference numbe: KY-20171981). No 
      consent was needed. CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: 
      The authors declare that they have no competing interests. PUBLISHER'S NOTE: 
      Springer Nature remains neutral with regard to jurisdictional claims in published 
      maps and institutional affiliations.
EDAT- 2018/08/22 06:00
MHDA- 2018/12/12 06:00
PMCR- 2018/08/21
CRDT- 2018/08/22 06:00
PHST- 2018/02/26 00:00 [received]
PHST- 2018/07/30 00:00 [accepted]
PHST- 2018/08/22 06:00 [entrez]
PHST- 2018/08/22 06:00 [pubmed]
PHST- 2018/12/12 06:00 [medline]
PHST- 2018/08/21 00:00 [pmc-release]
AID - 10.1186/s13000-018-0733-9 [pii]
AID - 733 [pii]
AID - 10.1186/s13000-018-0733-9 [doi]
PST - epublish
SO  - Diagn Pathol. 2018 Aug 21;13(1):56. doi: 10.1186/s13000-018-0733-9.