PMID- 30126531
OWN - NLM
STAT- MEDLINE
DCOM- 20191206
LR  - 20191217
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 31
IP  - 2
DP  - 2019 Jan
TI  - Long non-coding RNA HOX transcript antisense RNA (HOTAIR) suppresses the 
      angiogenesis of human placentation by inhibiting vascular endothelial growth 
      factor A expression.
PG  - 377-385
LID - 10.1071/RD18118 [doi]
AB  - HOX transcript antisense RNA (HOTAIR) is a long non-coding RNA located within the 
      Homeobox C (HOXC) gene cluster on chromosome 12. Previous studies have revealed 
      that HOTAIR is overexpressed in many types of cancers and is associated with 
      metastasis and poor survival rates; however, few reports have mentioned the 
      relationship between HOTAIR and angiogenesis of the human placenta. The aim of 
      the present study was to investigate the correlation between HOTAIR and vascular 
      endothelial growth factor (VEGF) A in the human placenta. HOTAIR levels decreased 
      significantly in human placenta with increasing gestational age, and were 
      negatively correlated with VEGFA levels. Invitro assays revealed that HOTAIR 
      overexpression suppressed the proliferation, migration, invasion and tube 
      formation of human umbilical vein endothelial cells (HUVECs); however, inhibition 
      of HOTAIR had the opposite effects. Furthermore, VEGFA overexpression reversed 
      the inhibitory effect of HOTAIR on the proliferation, migration, invasion and 
      tube formation of HUVECs. In addition, overexpression of HOTAIR significantly 
      inhibited VEGFA expression. Notably, a luciferase reporter assay found that 
      HOTAIR inhibited VEGFA transcription by directly targeting the VEGFA promoter. 
      Together, these results suggest that HOTAIR plays an important role in 
      suppressing angiogenesis of the human placenta by inhibiting the expression of 
      VEGFA; thus, HOTAIR may represent a potential therapeutic target for patients 
      with human placental vascularisation abnormalities.
FAU - Wu, Kejia
AU  - Wu K
AD  - Department of Gynecology, Zhongnan Hospital of Wuhan University, No. 169 South of 
      Donghu Road, Wuchang District, Wuhan 430071, Hubei, China.
FAU - Liu, Fulin
AU  - Liu F
AD  - The First Department of Gynecology, Renmin Hospital of Wuhan University, No. 238 
      Jiefang Road, Wuchang District, Wuhan 430060, Hubei, China.
FAU - Wu, Wanrong
AU  - Wu W
AD  - The First Department of Gynecology, Renmin Hospital of Wuhan University, No. 238 
      Jiefang Road, Wuchang District, Wuhan 430060, Hubei, China.
FAU - Chen, Yurou
AU  - Chen Y
AD  - The First Department of Gynecology, Renmin Hospital of Wuhan University, No. 238 
      Jiefang Road, Wuchang District, Wuhan 430060, Hubei, China.
FAU - Wu, Hanshu
AU  - Wu H
AD  - The First Department of Gynecology, Renmin Hospital of Wuhan University, No. 238 
      Jiefang Road, Wuchang District, Wuhan 430060, Hubei, China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Department of Gynecology, Zhongnan Hospital of Wuhan University, No. 169 South of 
      Donghu Road, Wuchang District, Wuhan 430071, Hubei, China.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (HOTAIR long untranslated RNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Female
MH  - Gene Expression Regulation
MH  - Gene Knockdown Techniques
MH  - Gestational Age
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Neovascularization, Physiologic/*genetics
MH  - Placentation/*genetics
MH  - Pregnancy
MH  - Promoter Regions, Genetic
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Vascular Endothelial Growth Factor A/genetics/*metabolism
EDAT- 2018/08/22 06:00
MHDA- 2019/12/18 06:00
CRDT- 2018/08/22 06:00
PHST- 2017/07/17 00:00 [received]
PHST- 2018/07/16 00:00 [accepted]
PHST- 2018/08/22 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2018/08/22 06:00 [entrez]
AID - RD18118 [pii]
AID - 10.1071/RD18118 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2019 Jan;31(2):377-385. doi: 10.1071/RD18118.