PMID- 30128741
OWN - NLM
STAT- MEDLINE
DCOM- 20181022
LR  - 20220410
IS  - 1432-2242 (Electronic)
IS  - 0040-5752 (Linking)
VI  - 131
IP  - 11
DP  - 2018 Nov
TI  - Physical mapping of the blue-grained gene from Thinopyrum ponticum chromosome 4Ag 
      and development of blue-grain-related molecular markers and a FISH probe based on 
      SLAF-seq technology.
PG  - 2359-2370
LID - 10.1007/s00122-018-3158-7 [doi]
AB  - A Thinopyrum ponticum chromosome 4Ag physical map was constructed, the 
      blue-grained gene was localized, and related specific markers and a FISH probe 
      were developed by SLAF-seq. Decaploid Thinopyrum ponticum (2n = 10x = 70) serves 
      as an important gene pool for wheat improvement. The wheat-Th. ponticum 4Ag (4D) 
      disomic substitution line Blue 58, derived from a distant hybridization between 
      Th. ponticum and common wheat (Triticum aestivum L.), bears blue coloration in 
      the aleurone layer. To map the blue-grained gene, eight wheat-Th. ponticum 4Ag 
      translocation lines with different chromosomal segment sizes were obtained from 
      Blue 58 using (60)Co-gamma ray irradiation and were characterized using cytogenetic 
      and molecular marker analysis. A small-segment blue-grained wheat translocation 
      line L13, accounting for one-fifth of 4AgL, was obtained. A physical map of 
      chromosome 4Ag was constructed containing 573 specific-locus amplified fragment 
      sequencing (SLAF-seq) markers, including three bins with 223 markers on 4AgS and 
      eight bins with 350 markers on 4AgL. The blue-grained gene in three blue-grained 
      translocation lines L5, L9, and L13, was located on bin 4AgL-6 with FL 0.75-0.89. 
      Moreover, 89 blue-grain-related molecular markers and one fluorescence in situ 
      hybridization (FISH) probe, pThp12.19, were identified in this bin. The newly 
      developed translocation lines and the molecular markers and FISH probe will 
      facilitate the application of the Th. ponticum-origin blue-grained characteristic 
      in wheat breeding.
FAU - Liu, Liqin
AU  - Liu L
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, 
      China.
FAU - Luo, Qiaoling
AU  - Luo Q
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, 
      China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Li, Hongwei
AU  - Li H
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, 
      China.
FAU - Li, Bin
AU  - Li B
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, 
      China.
FAU - Li, Zhensheng
AU  - Li Z
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, 
      China. zsli@genetics.ac.cn.
FAU - Zheng, Qi
AU  - Zheng Q
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, 
      China. qzheng@genetics.ac.cn.
LA  - eng
GR  - 2016YFD010200/the National Key Research and Development Program of China/
GR  - KEJ-STS-ZDTP-024/the STS Project of Chinese Academy of Science/
PT  - Journal Article
DEP - 20180820
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Chromosomes, Plant
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - *Physical Chromosome Mapping
MH  - Pigmentation/*genetics
MH  - Plant Breeding
MH  - Poaceae/*genetics
MH  - Translocation, Genetic
MH  - Triticum/genetics
EDAT- 2018/08/22 06:00
MHDA- 2018/10/23 06:00
CRDT- 2018/08/22 06:00
PHST- 2018/03/20 00:00 [received]
PHST- 2018/08/03 00:00 [accepted]
PHST- 2018/08/22 06:00 [pubmed]
PHST- 2018/10/23 06:00 [medline]
PHST- 2018/08/22 06:00 [entrez]
AID - 10.1007/s00122-018-3158-7 [pii]
AID - 10.1007/s00122-018-3158-7 [doi]
PST - ppublish
SO  - Theor Appl Genet. 2018 Nov;131(11):2359-2370. doi: 10.1007/s00122-018-3158-7. 
      Epub 2018 Aug 20.