PMID- 30129339
OWN - NLM
STAT- MEDLINE
DCOM- 20181114
LR  - 20210915
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 32
IP  - 9
DP  - 2018 Sep 15
TI  - [Study on antibacterial properties of titanium metallic surface due to 
      synergistic action of micro/nano-structure and antimicrobial peptides].
PG  - 1116-1122
LID - 10.7507/1002-1892.201805022 [doi]
AB  - OBJECTIVE: To investigate the effects of micro/nano-structure and antimicrobial 
      peptides (AMPs) on antibacterial properties for titanium (Ti) metal surface. 
      METHODS: Ti disks were treated via sandblasted large-grit acid-etched (SLA) and 
      alkali-heat treatment (AHT) to build the micor/nano-structure, on which AMPs were 
      spin-coated with a certain amount (10, 30, 50, 70, and 90 mug). Scanning electron 
      microscope (SEM) and energy dispersive spectroscopy (EDS) were used to observe 
      the surface structure and characterize the surface elements (i.e. contents of C, 
      N, O, and Ti). Ti disks loaded with AMPs of difference amounts were co-cultured 
      with Staphylococcus aureus ( S. aureus) for 24 hours. After that, the formation 
      and dimension of antibacterial circle were measured. Furthermore, the Ti disks 
      treated with different approaches (untreated, SLA treatment, SLA+THA treatment, 
      and loaded with 90 mug AMPs) were co-cultured with S. aureus and Escherichia coli 
      ( E.coli) for 3 hours, bacterial adhesion on the disks were evaluated by using 
      SEM. The antibacterial performances in solution were quantitatively evaluated by 
      immersing the Ti disks in bacterial solutions and measuring the absorbance ( A) 
      values. RESULTS: It was found that the nanoporous structure could be easily 
      constructed by SLA+AHT approach. After spin-coating AMPs, the nanopores with the 
      diameter less than 200 nm were almost covered. According to the element analysis, 
      with the increase of AMPs, the C content gradually increased; the N content was 
      not detected until AMPs amount reached 70 mug on the disks. The diameter of 
      antibacterial circle clearly depended on the AMPs amount. The Ti disks loaded 
      with 90 mug AMPs had significantly larger antibacterial circles than the other Ti 
      disks ( P<0.05). Based on the SEM observation, the Ti disks loaded with 90 mug 
      AMPs has the least bacterial attachment compared with the other Ti disks ( 
      P<0.05). The A value of bacterial solution immersed with the Ti disks loaded with 
      90 mug AMPs was much lower than the other Ti disks ( P<0.05). CONCLUSION: The 
      approach of micro/nano-structure and AMPs can improve the antibacterial 
      properties of Ti metal surface.
FAU - He, Hongyan
AU  - He H
AD  - Medical Biomaterials Engineering Research Center of the Ministry of Education, 
      East China University of Science and Technology, Shanghai, 200237, 
      P.R.China;State Key Laboratory of Bioreactor Engineering, East China University 
      of Science and Technology, Shanghai, 200237, P.R.China.
FAU - Gao, Pengyang
AU  - Gao P
AD  - Medical Biomaterials Engineering Research Center of the Ministry of Education, 
      East China University of Science and Technology, Shanghai, 200237, P.R.China.
FAU - Qiao, Zhongqian
AU  - Qiao Z
AD  - Medical Biomaterials Engineering Research Center of the Ministry of Education, 
      East China University of Science and Technology, Shanghai, 200237, P.R.China.
FAU - Qu, Xue
AU  - Qu X
AD  - Medical Biomaterials Engineering Research Center of the Ministry of Education, 
      East China University of Science and Technology, Shanghai, 200237, P.R.China.
FAU - Liu, Changsheng
AU  - Liu C
AD  - Medical Biomaterials Engineering Research Center of the Ministry of Education, 
      East China University of Science and Technology, Shanghai, 200237, 
      P.R.China;State Key Laboratory of Bioreactor Engineering, East China University 
      of Science and Technology, Shanghai, 200237, P.R.China.liucs@ecust.edu.cn.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Peptides)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Anti-Bacterial Agents
MH  - Bacterial Adhesion
MH  - *Nanostructures
MH  - Peptides
MH  - Staphylococcus aureus
MH  - Surface Properties
MH  - Titanium
PMC - PMC8413986
OTO - NOTNLM
OT  - Titanium
OT  - antibacterial peptides
OT  - antibacterial properties
OT  - implant material
OT  - micro/nano-structure
EDAT- 2018/08/22 06:00
MHDA- 2018/11/15 06:00
PMCR- 2018/09/01
CRDT- 2018/08/22 06:00
PHST- 2018/08/22 06:00 [entrez]
PHST- 2018/08/22 06:00 [pubmed]
PHST- 2018/11/15 06:00 [medline]
PHST- 2018/09/01 00:00 [pmc-release]
AID - zgxfcjwkzz-32-9-1116 [pii]
AID - 10.7507/1002-1892.201805022 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Sep 15;32(9):1116-1122. doi: 
      10.7507/1002-1892.201805022.