PMID- 30132528
OWN - NLM
STAT- MEDLINE
DCOM- 20190109
LR  - 20190109
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 18
IP  - 4
DP  - 2018 Oct
TI  - Isolation and characterization of minipig perivascular stem cells for bone tissue 
      engineering.
PG  - 3555-3562
LID - 10.3892/mmr.2018.9410 [doi]
AB  - Human subcutaneous adipose tissue has been recognized as a rich source of tissue 
      resident mesenchymal stem/stromal cells (MSC) in recent years. The current study 
      was designed to sort the minipig (mp) perivascular stem cells (PSCs) and 
      investigate the osteogenic potential. Purification of human PSCs was achieved via 
      fluorescence‑activated cell sorting (FACS) from human liposuction samples 
      [cluster of differentiation (CD)45‑CD34‑CD146+ perithelial cells and 
      CD45‑CD34+CD146‑ adventitial cells]. Subsequently, PSCs were isolated from mp 
      adipose tissue samples (n=9), characterized and, using purified mpPSCs (obtained 
      by FACS, which is used in human PSC purification), the mpPSC osteogenic and 
      adipogenic potential was evaluated by Alizarin Red S and Oil Red O staining 
      in vitro, respectively. The cell morphometry was observed following cell 
      isolation and culture, and hematoxylin and eosin staining was performed to 
      identify the fat tissue structure and vascular distribution. Osteogenic and 
      adipogenic differentiation‑associated gene expression levels were analyzed by 
      reverse transcription‑quantitative polymerase chain reaction. The results 
      demonstrated that the same antigens used for human PSC identification and 
      isolation were working in mp tissue (CD45, CD146 and CD34). The two cell groups: 
      CD45‑CD34‑CD146+ pericytes and CD45‑CD34+CD146‑ adventitial cells were 
      successfully isolated from the subcutaneous fat in the posterior neck of mps, 
      mpPSCs accounted for 8.6% of the stromal vascular fraction (SVF) with 1.4% 
      pericytes and 7.2% adventitial cells. mpPSCs demonstrated characteristics of 
      MSCs, including cell surface marker expression, colony forming unit‑fibroblast 
      inclusion, and the stronger osteogenic and adipogenic differentiation potential 
      than that of the non‑selected vascular stromal cells. The mRNA expression levels 
      of osteocalcin, collagen, type I, alpha1 and peroxisome proliferator‑activated 
      receptor‑gamma in the mpPSCs group were significantly higher than those of the 
      unsorted pSVF group (P<0.05). Thus, the current study successfully isolated and 
      cultured CD146+ and CD34+ cell populations from mp tissues, characterized the 
      cells' PSC‑like phenotype and identified their distinctly osteogenic and 
      adipogenic potential.
FAU - Cui, Zhen
AU  - Cui Z
AD  - Department of Orthodontics, Peking University School of Stomatology, Beijing 
      100081, P.R. China.
FAU - Li, Chenshuang
AU  - Li C
AD  - Department of Orthodontics, Peking University School of Stomatology, Beijing 
      100081, P.R. China.
FAU - Jiang, Nan
AU  - Jiang N
AD  - Central Laboratory, Peking University School of Stomatology, Beijing 100081, P.R. 
      China.
FAU - Zhang, Ci
AU  - Zhang C
AD  - Department of Orthodontics, Peking University School of Stomatology, Beijing 
      100081, P.R. China.
FAU - Wang, Yiran
AU  - Wang Y
AD  - Department of Orthodontics, Peking University School of Stomatology, Beijing 
      100081, P.R. China.
FAU - Gao, Hongyun
AU  - Gao H
AD  - Surgical Operating Room, Beijing Huangsi Plastic Surgery Hospital, Beijing 
      100020, P.R. China.
FAU - Zhou, Yanheng
AU  - Zhou Y
AD  - Department of Orthodontics, Peking University School of Stomatology, Beijing 
      100081, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20180821
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Antigens, CD34)
RN  - 0 (CD146 Antigen)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Adipogenesis
MH  - Animals
MH  - Antigens, CD34/analysis
MH  - CD146 Antigen/analysis
MH  - Cell Separation/*methods
MH  - Flow Cytometry/methods
MH  - Humans
MH  - Leukocyte Common Antigens/analysis
MH  - Male
MH  - Mesenchymal Stem Cells/cytology
MH  - Osteogenesis
MH  - Stem Cells/*cytology
MH  - Swine
MH  - Swine, Miniature
MH  - Tissue Engineering/methods
PMC - PMC6131542
EDAT- 2018/08/23 06:00
MHDA- 2019/01/10 06:00
PMCR- 2018/08/21
CRDT- 2018/08/23 06:00
PHST- 2017/07/13 00:00 [received]
PHST- 2017/10/25 00:00 [accepted]
PHST- 2018/08/23 06:00 [pubmed]
PHST- 2019/01/10 06:00 [medline]
PHST- 2018/08/23 06:00 [entrez]
PHST- 2018/08/21 00:00 [pmc-release]
AID - mmr-18-04-3555 [pii]
AID - 10.3892/mmr.2018.9410 [doi]
PST - ppublish
SO  - Mol Med Rep. 2018 Oct;18(4):3555-3562. doi: 10.3892/mmr.2018.9410. Epub 2018 Aug 
      21.