PMID- 30132978
OWN - NLM
STAT- MEDLINE
DCOM- 20191125
LR  - 20191210
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 119
IP  - 12
DP  - 2018 Dec
TI  - CASTOR1 suppresses the progression of lung adenocarcinoma and predicts poor 
      prognosis.
PG  - 10186-10194
LID - 10.1002/jcb.27360 [doi]
AB  - As a naturally occurring inhibitor of mammalian target of rapamycin (mTOR), 
      accumulated evidence has confirmed that CASTOR1 plays a pivotal role in 
      regulating the progression of human malignancies. However, the function of 
      CASTOR1 in the development of lung adenocarcinoma is still unclear. Here we 
      report that the expression of CASTOR1 is significantly reduced in lung 
      adenocarcinoma cell lines as compared with that in normal lung epithelial cells. 
      Cellular studies further revealed that ectopic CASTOR1 expression led to a 
      significant suppression of the cellular proliferation, migration, and invasion of 
      PC-9 cells. To further test the role of CASTOR1 in human patients with lung 
      cancers, tumor tissues derived from lung cancer patients and paired adjacent 
      normal lung tissues were collected for immunohistochemical​​​​​​, biochemical, 
      and molecular biology analysis. Immunoblotting and real-time quantitative reverse 
      transcription polymerase chain reaction analysis is revealed that CASTOR1 was 
      significantly reduced in tumor tissues as compared with that in paired normal 
      lung tissues. With immunostaining and retrospective analysis of pathological 
      samples from lung cancer patients further revealed that expression of CASTOR1 was 
      negatively correlated with smoking history, TNM stage, lymphnode metastasis, and 
      distant metastasis. Furthermore, Kaplan-Meier survival analysis indicated that 
      patients with low CASTOR1 expression levels had a significantly worse 5-year 
      survival rate than those with high CASTOR1 expression. To further explore the 
      molecular actions of CASTOR1 in lung cancer development, biochemical assays were 
      performed and the results suggested that ectopic CASTOR1 expression resulted 
      significant suppression of mTOR and downstream S6K phosphorylation, indicating 
      that CASTOR1 might regulate the progression of lung adenocarcinoma by controlling 
      mTOR activation. Thus, these findings demonstrated that CASTOR1 inhibits the 
      tumorigenesis of lung adenocarcinoma cells and might serve as a potential 
      therapeutic target or prognostic marker for human patients with lung 
      adenocarcinoma.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Zhou, Xuefeng
AU  - Zhou X
AD  - Department of Thoracic and Cardiovascular Surgery, Zhongnan Hospital, Wuhan 
      University, Wuhan, China.
FAU - Cheng, Zhenshun
AU  - Cheng Z
AD  - Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University, Wuhan, 
      China.
FAU - Chen, Hao
AU  - Chen H
AD  - Department of Thoracic and Cardiovascular Surgery, Zhongnan Hospital, Wuhan 
      University, Wuhan, China.
FAU - Shi, Shenqi
AU  - Shi S
AD  - Department of Surgery, Dawu People's Hospital, China.
FAU - Wang, Xianguo
AU  - Wang X
AD  - Department of Thoracic and Cardiovascular Surgery, Zhongnan Hospital, Wuhan 
      University, Wuhan, China.
FAU - Orang, Matthew
AU  - Orang M
AD  - Department of Physical Education & Human Performance, Central Connecticut State 
      University, New Britain, Connecticut.
FAU - Zhao, Jinping
AU  - Zhao J
AUID- ORCID: 0000-0002-4699-6240
AD  - Department of Thoracic and Cardiovascular Surgery, Zhongnan Hospital, Wuhan 
      University, Wuhan, China.
LA  - eng
PT  - Journal Article
DEP - 20180821
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (CASTOR1 protein, human)
RN  - 0 (Carrier Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
SB  - IM
MH  - Adenocarcinoma of Lung/*genetics/pathology
MH  - Aged
MH  - Carcinogenesis/*genetics
MH  - Carrier Proteins/*genetics
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Disease Progression
MH  - Disease-Free Survival
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Kaplan-Meier Estimate
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - Neoplasm Staging
MH  - *Prognosis
MH  - Signal Transduction
OTO - NOTNLM
OT  - CASTOR1
OT  - lung adenocarcinoma
OT  - mammalian target of rapamycin
OT  - proliferation
EDAT- 2018/08/23 06:00
MHDA- 2019/11/26 06:00
CRDT- 2018/08/23 06:00
PHST- 2017/11/25 00:00 [received]
PHST- 2018/06/26 00:00 [accepted]
PHST- 2018/08/23 06:00 [pubmed]
PHST- 2019/11/26 06:00 [medline]
PHST- 2018/08/23 06:00 [entrez]
AID - 10.1002/jcb.27360 [doi]
PST - ppublish
SO  - J Cell Biochem. 2018 Dec;119(12):10186-10194. doi: 10.1002/jcb.27360. Epub 2018 
      Aug 21.