PMID- 30133262
OWN - NLM
STAT- MEDLINE
DCOM- 20190729
LR  - 20201209
IS  - 1520-5010 (Electronic)
IS  - 0893-228X (Linking)
VI  - 31
IP  - 9
DP  - 2018 Sep 17
TI  - Quantification of Microcystin-LR in Human Urine by Immunocapture Liquid 
      Chromatography Tandem Mass Spectrometry.
PG  - 898-903
LID - 10.1021/acs.chemrestox.8b00126 [doi]
AB  - Microcystins are toxins produced by many cyanobacteria species, which are often 
      released into waterways during blue-green algal blooms in freshwater and marine 
      habitats. The consumption of microcystin-contaminated water is a public health 
      concern as these toxins are recognized tumor promoters and are hepatotoxic to 
      humans and animals. A method to confirm human exposures to microcystins is 
      needed; therefore, our laboratory has developed an immunocapture liquid 
      chromatography tandem mass spectrometry (LC-MS/MS) method targeting the conserved 
      adda portion of microcystins for the quantitation of a prevalent and highly toxic 
      congener of microcystin, microcystin-LR (MC-LR). An acute exposure method was 
      initially evaluated for accuracy and precision by analyzing calibrators and 
      quality control (QC) samples ranging from 0.500 to 75.0 ng/mL in urine. All 
      calibrators and QC samples characterized were within 15% of theoretical 
      concentrations. An analysis of acutely exposed mouse urine samples using this 
      method identified MC-LR levels from 10.7 to 33.9 ng/mL. Since human exposures are 
      anticipated to result from low-dose or chronic exposures, a high-sensitivity 
      method was validated with 20 calibration curves and QC samples ranging from 
      0.0100 to 7.50 ng/mL. Relative standard deviations (RSDs) and inaccuracies of 
      these samples were within 15%, meeting United States Food and Drug Administration 
      (FDA) guidelines for analytical methods, and the limit of detection was 0.00455 
      ng/mL. In conclusion, we have developed a method which can be used to address 
      public health concerns by precisely and accurately measuring MC-LR in urine 
      samples.
FAU - Wharton, Rebekah E
AU  - Wharton RE
AUID- ORCID: 0000-0003-3027-3811
FAU - Ojeda-Torres, Geovannie
AU  - Ojeda-Torres G
FAU - Cunningham, Brady
AU  - Cunningham B
FAU - Feyereisen, Melanie C
AU  - Feyereisen MC
FAU - Hill, Kasey L
AU  - Hill KL
FAU - Abbott, Nicole L
AU  - Abbott NL
FAU - Seymour, Craig
AU  - Seymour C
FAU - Hill, Donna
AU  - Hill D
FAU - Lang, Johnsie
AU  - Lang J
FAU - Hamelin, Elizabeth I
AU  - Hamelin EI
AUID- ORCID: 0000-0003-4564-040X
FAU - Johnson, Rudolph C
AU  - Johnson RC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20180905
PL  - United States
TA  - Chem Res Toxicol
JT  - Chemical research in toxicology
JID - 8807448
RN  - 0 (Marine Toxins)
RN  - 0 (Microcystins)
RN  - EQ8332842Y (cyanoginosin LR)
MH  - Animals
MH  - Chromatography, Liquid/*methods
MH  - Cyanobacteria/metabolism
MH  - Female
MH  - Humans
MH  - Limit of Detection
MH  - Male
MH  - Marine Toxins
MH  - Mice
MH  - Microcystins/*urine
MH  - Quality Control
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/methods
EDAT- 2018/08/23 06:00
MHDA- 2019/07/30 06:00
CRDT- 2018/08/23 06:00
PHST- 2018/08/23 06:00 [pubmed]
PHST- 2019/07/30 06:00 [medline]
PHST- 2018/08/23 06:00 [entrez]
AID - 10.1021/acs.chemrestox.8b00126 [doi]
PST - ppublish
SO  - Chem Res Toxicol. 2018 Sep 17;31(9):898-903. doi: 10.1021/acs.chemrestox.8b00126. 
      Epub 2018 Sep 5.