PMID- 30133747
OWN - NLM
STAT- MEDLINE
DCOM- 20200114
LR  - 20231213
IS  - 1469-8137 (Electronic)
IS  - 0028-646X (Linking)
VI  - 221
IP  - 2
DP  - 2019 Jan
TI  - Mitochondrial complex I dysfunction increases CO(2) efflux and reconfigures 
      metabolic fluxes of day respiration in tobacco leaves.
PG  - 750-763
LID - 10.1111/nph.15393 [doi]
AB  - Mutants affected in complex I are useful to understand the role played by 
      mitochondrial electron transport and redox metabolism in cellular homeostasis and 
      signaling. However, their respiratory phenotype is incompletely described and a 
      specific examination of day respiration (R(d) ) is lacking. Here, we used 
      isotopic methods and metabolomics to investigate the impact of complex I 
      dysfunction on R(d) in two respiratory mutants of forest tobacco (Nicotiana 
      sylvestris): cytoplasmic male sterile II (CMSII) and nuclear male sterile 1 
      (NMS1), previously characterized for complex I disruption. R(d) was higher in 
      mutants and the inhibition of leaf respiration by light was lower. Higher R(d) 
      values were caused by increased (phosphoenol)pyruvate (PEP) metabolism at the 
      expense of anaplerotic (PEP carboxylase (PEPc) -catalyzed) activity. De novo 
      synthesis of Krebs cycle intermediates in the light was larger in mutants than in 
      the wild-type, although numerically small in all genotypes. Carbon metabolism in 
      mutants involved alternative pathways, such as alanine synthesis, and an increase 
      in amino acid production with the notable exception of aspartate. Our results 
      show that the alteration of NADH re-oxidation activity by complex I does not 
      cause a general inhibition of catabolism, but rather a re-orchestration of fluxes 
      in day respiratory metabolism, leading to an increased CO(2) efflux.
CI  - (c) 2018 The Authors. New Phytologist (c) 2018 New Phytologist Trust.
FAU - Lothier, Jeremy
AU  - Lothier J
AD  - Institut de Recherche en Horticulture et Semences, UMR 1345 INRA-Universite 
      d'Angers, 42 rue Georges Morel, 49071, Beaucouze Cedex, France.
FAU - De Paepe, Rosine
AU  - De Paepe R
AD  - Institute of Plant Sciences Paris-Saclay, UMR 9213/UMR1403, Universite Paris Sud, 
      CNRS-INRA, Universite d'Evry, Universite Paris-Diderot, Batiment 630, 91405, 
      Orsay Cedex, France.
FAU - Tcherkez, Guillaume
AU  - Tcherkez G
AD  - Research School of Biology, Australian National University, ANU College of 
      Science, 2601, Canberra, ACT, Australia.
LA  - eng
GR  - FT140100645/Australian Research Council/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180822
PL  - England
TA  - New Phytol
JT  - The New phytologist
JID - 9882884
RN  - 0 (Carbon Isotopes)
RN  - 0 (Gases)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 8558G7RUTR (Pyruvic Acid)
RN  - EC 7.1.1.2 (Electron Transport Complex I)
RN  - FDJ0A8596D (Carbon-13)
SB  - IM
MH  - Carbon Dioxide/*metabolism
MH  - Carbon Isotopes
MH  - Cell Respiration
MH  - Decarboxylation
MH  - Electron Transport Complex I/*metabolism
MH  - Gases/metabolism
MH  - *Metabolic Flux Analysis
MH  - Metabolome
MH  - Metabolomics
MH  - Mitochondria/*metabolism
MH  - Plant Leaves/*metabolism
MH  - Pyruvic Acid/metabolism
MH  - Nicotiana/*cytology/*metabolism
OTO - NOTNLM
OT  - CO2 efflux
OT  - day respiration (Rd)
OT  - isotopic labeling
OT  - photorespiration
OT  - tricarboxylic acid cycle
EDAT- 2018/08/23 06:00
MHDA- 2020/01/15 06:00
CRDT- 2018/08/23 06:00
PHST- 2018/05/20 00:00 [received]
PHST- 2018/07/15 00:00 [accepted]
PHST- 2018/08/23 06:00 [pubmed]
PHST- 2020/01/15 06:00 [medline]
PHST- 2018/08/23 06:00 [entrez]
AID - 10.1111/nph.15393 [doi]
PST - ppublish
SO  - New Phytol. 2019 Jan;221(2):750-763. doi: 10.1111/nph.15393. Epub 2018 Aug 22.