PMID- 30135552
OWN - NLM
STAT- MEDLINE
DCOM- 20191021
LR  - 20191022
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Aug 22
TI  - Assessment of Corneal Stromal Remodeling and Regeneration after Photorefractive 
      Keratectomy.
PG  - 12580
LID - 10.1038/s41598-018-30372-2 [doi]
LID - 12580
AB  - This study utilizes high resolution multi-dimensional imaging to identify 
      temporal and spatial changes in cell/extracellular matrix (ECM) patterning 
      mediating cell migration, fibrosis, remodeling and regeneration during wound 
      healing. Photorefractive keratectomy (PRK) was performed on rabbits. In some 
      cases, 5([4,6-dichlorotriazin-2yl]-amino)fluorescein (DTAF) was applied 
      immediately after surgery to differentiate native vs. cell-secreted collagen. 
      Corneas were assessed 3-180 days postoperatively using in vivo confocal 
      microscopy, and cell/ECM patterning was evaluated in situ using multiphoton and 
      second harmonic generation (SHG) imaging. 7 days post-PRK, migrating fibroblasts 
      below the ablation site were co-aligned with the stromal lamellae. At day 21, 
      randomly patterned myofibroblasts developed on top of the ablation site; whereas 
      cells underneath were elongated, co-aligned with collagen, and lacked stress 
      fibers. Over time, fibrotic tissue was remodeled into more transparent stromal 
      lamellae. By day 180, stromal thickness was almost completely restored. Stromal 
      regrowth occurred primarily below the ablation interface, and was characterized 
      by co-localization of gaps in DTAF labeling with elongated cells and SHG collagen 
      signaling. Punctate F-actin labeling was detected along cells co-aligned with 
      DTAF and non-DTAF labeled collagen, suggesting cell-ECM interactions. Overall, 
      collagen lamellae appear to provide a template for fibroblast patterning during 
      wound healing that mediates stromal repopulation, regeneration and remodeling.
FAU - Kivanany, Pouriska B
AU  - Kivanany PB
AD  - Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, USA.
AD  - Biomedical Engineering Graduate Program, UT Southwestern Medical Center, Dallas, 
      TX, USA.
FAU - Grose, Kyle C
AU  - Grose KC
AD  - Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, USA.
FAU - Tippani, Madhavi
AU  - Tippani M
AD  - Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, USA.
FAU - Su, Shan
AU  - Su S
AD  - Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, USA.
FAU - Petroll, W Matthew
AU  - Petroll WM
AD  - Department of Ophthalmology, UT Southwestern Medical Center, Dallas, TX, USA. 
      matthew.petroll@utsouthwestern.edu.
AD  - Biomedical Engineering Graduate Program, UT Southwestern Medical Center, Dallas, 
      TX, USA. matthew.petroll@utsouthwestern.edu.
LA  - eng
GR  - P30 EY020799/EY/NEI NIH HHS/United States
GR  - R01 EY013322/EY/NEI NIH HHS/United States
GR  - EY013322/U.S. Department of Health & Human Services | NIH | National Eye 
      Institute (NEI)/International
PT  - Journal Article
DEP - 20180822
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Corneal Stroma/*physiology/*surgery
MH  - Extracellular Matrix/metabolism
MH  - *Photorefractive Keratectomy
MH  - Rabbits
MH  - *Regeneration
MH  - Time Factors
MH  - Wound Healing
PMC - PMC6105640
COIS- The authors declare no competing interests.
EDAT- 2018/08/24 06:00
MHDA- 2019/10/23 06:00
PMCR- 2018/08/22
CRDT- 2018/08/24 06:00
PHST- 2018/04/13 00:00 [received]
PHST- 2018/07/16 00:00 [accepted]
PHST- 2018/08/24 06:00 [entrez]
PHST- 2018/08/24 06:00 [pubmed]
PHST- 2019/10/23 06:00 [medline]
PHST- 2018/08/22 00:00 [pmc-release]
AID - 10.1038/s41598-018-30372-2 [pii]
AID - 30372 [pii]
AID - 10.1038/s41598-018-30372-2 [doi]
PST - epublish
SO  - Sci Rep. 2018 Aug 22;8(1):12580. doi: 10.1038/s41598-018-30372-2.