PMID- 30136236
OWN - NLM
STAT- MEDLINE
DCOM- 20190528
LR  - 20200528
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 1857
DP  - 2018
TI  - Analyzing Necroptosis Using an RIPK1 Kinase Inactive Mouse Model of TNF Shock.
PG  - 125-134
LID - 10.1007/978-1-4939-8754-2_12 [doi]
AB  - The serine/threonine kinase RIPK1 has numerous biological and pathological 
      functions, mediating prosurvival as well as prodeath apoptotic and necroptotic 
      signaling pathways downstream of various receptors, including death receptors and 
      Toll-like receptors (TLRs). RIPK1 has been implicated in various diseases, 
      including ischemia-reperfusion injury and inflammatory bowel disease (IBD). The 
      recent generation of RIPK1 kinase inactive mice has enabled us to genetically 
      interrogate the role of RIPK1 kinase-mediated necroptosis in disease models. 
      Here, we describe procedures utilizing kinase inactive Ripk1(D138N/D138N) mice to 
      analyze necroptosis induction in vitro in bone-marrow derived macrophages (BMDMs) 
      and in vivo in a murine model of TNF-induced shock.
FAU - Zelic, Matija
AU  - Zelic M
AD  - Department of Molecular, Cell and Cancer Biology, University of Massachusetts 
      Medical School, Worcester, MA, USA.
FAU - Kelliher, Michelle A
AU  - Kelliher MA
AD  - Department of Molecular, Cell and Cancer Biology, University of Massachusetts 
      Medical School, Worcester, MA, USA. michelle.kelliher@umassmed.edu.
LA  - eng
GR  - R01 AI075118/AI/NIAID NIH HHS/United States
GR  - R56 AI075118/AI/NIAID NIH HHS/United States
GR  - T32 AI095213/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (Ripk1 protein, mouse)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cells, Cultured
MH  - *Disease Models, Animal
MH  - Female
MH  - Macrophages/drug effects/*pathology
MH  - Male
MH  - Mice
MH  - *Necrosis
MH  - Receptor-Interacting Protein Serine-Threonine Kinases/*antagonists & 
      inhibitors/genetics
MH  - Shock, Septic/chemically induced/*pathology
MH  - Tumor Necrosis Factor-alpha/*toxicity
PMC - PMC7250063
MID - NIHMS1587030
OTO - NOTNLM
OT  - Bone marrow-derived macrophages
OT  - Caspase inhibitor
OT  - Cell death
OT  - Lipopolysaccharide
OT  - Necroptosis
OT  - TNF shock
EDAT- 2018/08/24 06:00
MHDA- 2019/05/29 06:00
PMCR- 2020/05/26
CRDT- 2018/08/24 06:00
PHST- 2018/08/24 06:00 [entrez]
PHST- 2018/08/24 06:00 [pubmed]
PHST- 2019/05/29 06:00 [medline]
PHST- 2020/05/26 00:00 [pmc-release]
AID - 10.1007/978-1-4939-8754-2_12 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1857:125-134. doi: 10.1007/978-1-4939-8754-2_12.