PMID- 30136240
OWN - NLM
STAT- MEDLINE
DCOM- 20190528
LR  - 20190528
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1857
DP  - 2018
TI  - Characterization of the TNFR1-SC Using "Modified Tandem Affinity Purification" in 
      Conjunction with Liquid Chromatography-Mass Spectrometry (LC-MS).
PG  - 161-169
LID - 10.1007/978-1-4939-8754-2_16 [doi]
AB  - Mass spectrometry enables the unbiased characterization of protein complexes. The 
      success of this approach and the amount of information that can be retrieved are 
      highly dependent on the achieved purity of the protein complex to be analyzed. 
      Here we describe a modified tandem affinity purification (moTAP) approach which 
      can be used to isolate the tumor necrosis factor receptor 1 signaling complex for 
      subsequent analysis by liquid chromatography-tandem mass spectrometry. Indeed, 
      this approach can easily be adapted to the isolation of other membrane-bound and 
      intracellular signaling complexes. This methodology allows for a highly sensitive 
      analysis and characterization of complex components, including posttranslational 
      modifications and for the identification of novel complex components.
FAU - Reichert, Matthias
AU  - Reichert M
AD  - Centre for Cell Death, Cancer and Inflammation (CCCI), UCL Cancer Institute, 
      University College London, London, UK.
FAU - Bhamra, Amandeep
AU  - Bhamra A
AD  - Proteomics Research Core Facility, UCL Cancer Institute, University College 
      London, London, UK.
FAU - Kupka, Sebastian
AU  - Kupka S
AD  - Centre for Cell Death, Cancer and Inflammation (CCCI), UCL Cancer Institute, 
      University College London, London, UK.
FAU - Walczak, Henning
AU  - Walczak H
AD  - Centre for Cell Death, Cancer and Inflammation (CCCI), UCL Cancer Institute, 
      University College London, London, UK. h.walczak@ucl.ac.uk.
LA  - eng
GR  - 096831/Z/11/Z/Wellcome Trust/United Kingdom
GR  - A17341/Cancer Research UK/United Kingdom
GR  - BB/J013129/1/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - 515818/Cancer Research UK/United Kingdom
GR  - 525877/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Multiprotein Complexes)
RN  - 0 (Receptors, Tumor Necrosis Factor, Type I)
SB  - IM
MH  - Chromatography, Affinity/*methods
MH  - Chromatography, Liquid/*methods
MH  - Cross-Linking Reagents/chemistry
MH  - Humans
MH  - Mass Spectrometry/*methods
MH  - Multiprotein Complexes/isolation & purification/*metabolism
MH  - Receptors, Tumor Necrosis Factor, Type I/isolation & purification/*metabolism
OTO - NOTNLM
OT  - Immunoprecipitation
OT  - Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
OT  - Proteomics
OT  - TNF receptor 1 signaling complex (TNFR1-SC)
OT  - Tandem affinity purification
OT  - Tumor necrosis factor (TNF)
EDAT- 2018/08/24 06:00
MHDA- 2019/05/29 06:00
CRDT- 2018/08/24 06:00
PHST- 2018/08/24 06:00 [entrez]
PHST- 2018/08/24 06:00 [pubmed]
PHST- 2019/05/29 06:00 [medline]
AID - 10.1007/978-1-4939-8754-2_16 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2018;1857:161-169. doi: 10.1007/978-1-4939-8754-2_16.