PMID- 30137229
OWN - NLM
STAT- MEDLINE
DCOM- 20200207
LR  - 20200309
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 100
IP  - 1
DP  - 2019 Jan 1
TI  - Associations between fetal size, sex and placental angiogenesis in the pig.
PG  - 239-252
LID - 10.1093/biolre/ioy184 [doi]
AB  - Inadequate fetal growth cannot be remedied postnatally, leading to severe 
      consequences for neonatal and adult development. It is hypothesized that growth 
      restriction occurs due to inadequate placental vascularization. This study 
      investigated the relationship between porcine fetal size, sex, and placental 
      angiogenesis at multiple gestational days (GD). Placental samples supplying the 
      lightest and closest to mean litter weight (CTMLW), male and female Large White X 
      Landrace fetuses were obtained at GD30, 45, 60, and 90. Immunohistochemistry 
      revealed increased chorioallantoic membrane CD31 staining in placentas supplying 
      the lightest compared to those supplying the CTMLW fetuses at GD60. At GD90, 
      placentas supplying the lightest fetuses had decreased CD31 staining in the 
      chorioallantoic membrane compared to those supplying the CTMLW fetuses. The mRNA 
      expression of six candidate genes with central roles at the feto-maternal 
      interface increased with advancing gestation. At GD60, ACP5 expression was 
      increased in placentas supplying the lightest compared to the CTMLW fetuses. At 
      GD45, CD31 expression was decreased in placentas supplying the lightest compared 
      to the CTMLW fetuses. In contrast, CD31 expression was increased in placentas 
      supplying the lightest compared the CTMLW fetuses at GD60. In vitro endothelial 
      cell branching assays demonstrated that placentas supplying the lightest and male 
      fetuses impaired endothelial cell branching compared to placentas from the CTMLW 
      (GD45 and 60) and female fetuses (GD60), respectively. This study has highlighted 
      that placentas supplying the lightest and male fetuses have impaired 
      angiogenesis. Importantly, the relationship between fetal size, sex, and 
      placental vascularity is dynamic and dependent upon the GD investigated.
FAU - Stenhouse, Claire
AU  - Stenhouse C
AD  - Developmental Biology Division, The Roslin Institute and Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Midlothian, UK.
FAU - Hogg, Charis O
AU  - Hogg CO
AD  - Developmental Biology Division, The Roslin Institute and Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Midlothian, UK.
FAU - Ashworth, Cheryl J
AU  - Ashworth CJ
AD  - Developmental Biology Division, The Roslin Institute and Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Midlothian, UK.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
SB  - IM
MH  - Animals
MH  - Female
MH  - Fetal Development/physiology
MH  - Fetal Weight/*physiology
MH  - Fetus/physiology
MH  - Male
MH  - Neovascularization, Physiologic/*physiology
MH  - Placenta/*blood supply
MH  - Placentation/physiology
MH  - Pregnancy
MH  - *Pregnancy, Animal
MH  - *Sex Characteristics
MH  - Sex Factors
MH  - Swine/*physiology
PMC - PMC6335214
EDAT- 2018/08/24 06:00
MHDA- 2020/02/08 06:00
PMCR- 2018/08/18
CRDT- 2018/08/24 06:00
PHST- 2018/04/16 00:00 [received]
PHST- 2018/08/16 00:00 [accepted]
PHST- 2018/08/24 06:00 [pubmed]
PHST- 2020/02/08 06:00 [medline]
PHST- 2018/08/24 06:00 [entrez]
PHST- 2018/08/18 00:00 [pmc-release]
AID - 5076027 [pii]
AID - ioy184 [pii]
AID - 10.1093/biolre/ioy184 [doi]
PST - ppublish
SO  - Biol Reprod. 2019 Jan 1;100(1):239-252. doi: 10.1093/biolre/ioy184.