PMID- 30137616
OWN - NLM
STAT- MEDLINE
DCOM- 20190221
LR  - 20200220
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Linking)
VI  - 98
IP  - 1
DP  - 2019 Jan 1
TI  - Effects of dietary supplementation of DL-2-hydroxy-4(methylthio) butanoic acid on 
      antioxidant capacity and its related gene expression in lung and liver of 
      broilers exposed to low temperature.
PG  - 341-349
LID - 10.3382/ps/pey371 [doi]
AB  - DL-2-hydroxy-4(methylthio) butanoic acid (DL-HMTBA) exhibits a higher antioxidant 
      capability in vitro as compared to DL-Met, but the mechanism is still not known. 
      A total of 400 8-day-old broiler chicks were allotted to a 2 [low (12 to 14 degrees C) 
      vs. control temperature (thermoneutral, 24 to 26 degrees C)] x 2 (0.17% or 0.51% of 
      DL-HMTBA) factorial arrangement to investigate effects of DL-HMTBA on antioxidant 
      capacity and its related gene expression in lung and liver of broilers exposed to 
      low temperature. The hepatic glutathione (GSH), superoxide dismutase (SOD), and 
      glutathione peroxidase (GSH-Px) activities were decreased, whereas protein 
      carbonyl and malodndialdehyde contents in lung were increased in low temperature 
      (P < 0.05). Dietary supplementation of 0.51% DL-HMTBA increased (P < 0.05) GSH 
      and GSH-Px activity in liver and SOD activity in lung in spite of the 
      temperature. The greater gene expression of GSH reductase and lower expression of 
      GSH synthetase (P < 0.01) were observed in lung of broilers that were maintained 
      at low temperature. Higher DL-HMTBA supplementation induced greater (P < 0.05) 
      mRNA expression of glutathione-S transferase in lung, GSH synthetase in liver and 
      lung, as well as lower expression of GSH reductase in lung, and this effect were 
      more obvious for the chicks exposed to low temperature (interaction, P < 0.05). 
      Chicks that were maintained under low temperature had the lower expression of 
      cystathionine beta-synthase (CbetaS), whereas those fed 0.51% DL-HMTBA increased the 
      CbetaS expression in liver under low temperature (interaction, P </= 0.05). The 
      gamma-glutamylcysteine synthetase and Met adenosyltransferase 1 (MAT1) gene 
      expression were downregulated (P < 0.05) by low temperature and the higher (P = 
      0.02) expression of MAT1 was observed in lung of chicks fed 0.51% DL-HMTBA. It 
      indicated that under low temperature, DL-HMTBA supplementation at 0.51% 
      upregulated gene expression of GSH synthesis and Met transsulfuration pathway in 
      liver and lung to increase the antioxidant capacity, and then mitigate the 
      negative effects of cold stress for broilers.
FAU - Wang, Jianping
AU  - Wang J
AD  - Animal Nutrition Institute, Key Laboratory for Animal Disease-Resistance 
      Nutrition of China Ministry of Education, Sichuan Agricultural University, 
      Chengdu, Sichuan, 611130, China.
FAU - Yang, Geling
AU  - Yang G
AD  - Animal Nutrition Institute, Key Laboratory for Animal Disease-Resistance 
      Nutrition of China Ministry of Education, Sichuan Agricultural University, 
      Chengdu, Sichuan, 611130, China.
FAU - Zhang, Keying
AU  - Zhang K
AD  - Animal Nutrition Institute, Key Laboratory for Animal Disease-Resistance 
      Nutrition of China Ministry of Education, Sichuan Agricultural University, 
      Chengdu, Sichuan, 611130, China.
FAU - Ding, Xuemei
AU  - Ding X
AD  - Animal Nutrition Institute, Key Laboratory for Animal Disease-Resistance 
      Nutrition of China Ministry of Education, Sichuan Agricultural University, 
      Chengdu, Sichuan, 611130, China.
FAU - Bai, Shiping
AU  - Bai S
AD  - Animal Nutrition Institute, Key Laboratory for Animal Disease-Resistance 
      Nutrition of China Ministry of Education, Sichuan Agricultural University, 
      Chengdu, Sichuan, 611130, China.
FAU - Zeng, Qiufeng
AU  - Zeng Q
AD  - Animal Nutrition Institute, Key Laboratory for Animal Disease-Resistance 
      Nutrition of China Ministry of Education, Sichuan Agricultural University, 
      Chengdu, Sichuan, 611130, China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Antioxidants)
RN  - 583-91-5 (alpha-hydroxy-gamma-methylmercaptobutyric acid)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animal Feed/*analysis
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - Chickens/*physiology
MH  - Cold Temperature/*adverse effects
MH  - Diet/veterinary
MH  - Gene Expression
MH  - Liver/enzymology
MH  - Lung/enzymology
MH  - Male
MH  - Methionine/administration & dosage/*analogs & derivatives/pharmacology
EDAT- 2018/08/24 06:00
MHDA- 2019/02/23 06:00
CRDT- 2018/08/24 06:00
PHST- 2017/12/31 00:00 [received]
PHST- 2018/07/20 00:00 [accepted]
PHST- 2018/08/24 06:00 [pubmed]
PHST- 2019/02/23 06:00 [medline]
PHST- 2018/08/24 06:00 [entrez]
AID - S0032-5791(19)30316-5 [pii]
AID - 10.3382/ps/pey371 [doi]
PST - ppublish
SO  - Poult Sci. 2019 Jan 1;98(1):341-349. doi: 10.3382/ps/pey371.