PMID- 30140120 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220321 IS - 0972-7531 (Print) IS - 0976-3260 (Electronic) IS - 0972-7531 (Linking) VI - 25 IP - 2 DP - 2018 Jul TI - Alterations in MicroRNA-132/212 Expression Impairs Fear Memory in Goldfish Carassius auratus. PG - 90-97 LID - 10.1159/000486842 [doi] AB - BACKGROUND: Earlier, we showed that nicotinamide (NAM) treatment impairs spatial memory through the downregulation of CREB-Sirt 1-brain-derived neurotrophic factor (Bdnf) signaling cascade. PURPOSE: In this study, we examine whether NAM treatment alters CREB-regulated genes through -microRNAs. METHOD: To test this hypothesis, goldfish (Carassius auratus) were divided into 2 groups: (i) vehicle group (VEH; double distilled water intra-peritoneally [i.p.]) (ii) nicotinamide group (NAM, 1,000 mg/kg, i.p.) and again divided into VEH untrained/trained, NAM untrained/trained. One hour after receiving VEH or NAM, individuals were subject to contextual fear conditioning (CFC) training. After 24 h, both the groups were tested for contextual fear memory. Subsequently, miR-132/212 levels, regulated immediate-early genes (IEGs: C-fos and EGR-1) and Bdnf but not its receptor. -TrkB1were examined following 0' and 60' min after training, and compared with the untrained group. RESULTS: We observed that NAM treatment significantly impaired fear memory. Further, the analysis showed that miR-132 level was not altered, but miR-212 level was significantly upregulated after CFC training only in NAM-treated fish. We also found that NAM treatment downregulated IEGs and Bdnf but not its receptor TrkB1. CONCLUSIONS: Present study suggests that NAM-treatment impaired fear memory and control IEGs, Bdnf and TrkB1 expression by differentially regulating miR-132 and 212. FAU - Thangaleela, Subramanian AU - Thangaleela S AD - Behavioural Neuroscience Laboratory, Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India. FAU - Shanmugapriya, Vasudevan AU - Shanmugapriya V AD - Behavioural Neuroscience Laboratory, Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India. FAU - Mukilan, Murugan AU - Mukilan M AD - Behavioural Neuroscience Laboratory, Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India. FAU - Radhakrishnan, Karuppasamy AU - Radhakrishnan K AD - Department of Zoology, Government Arts College, Karur, India. FAU - Rajan, Koilmani Emmanuvel AU - Rajan KE AD - Behavioural Neuroscience Laboratory, Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India. LA - eng PT - Journal Article DEP - 20180202 PL - United States TA - Ann Neurosci JT - Annals of neurosciences JID - 101523367 PMC - PMC6103369 OTO - NOTNLM OT - Brain-derived neurotrophic factor OT - Contextual fear conditioning OT - Goldfish OT - Immediate-early genes OT - MicroRNAs-132/212 OT - Nicotinamide EDAT- 2018/08/25 06:00 MHDA- 2018/08/25 06:01 PMCR- 2018/02/02 CRDT- 2018/08/25 06:00 PHST- 2017/09/11 00:00 [received] PHST- 2018/01/15 00:00 [accepted] PHST- 2018/08/25 06:00 [entrez] PHST- 2018/08/25 06:00 [pubmed] PHST- 2018/08/25 06:01 [medline] PHST- 2018/02/02 00:00 [pmc-release] AID - aon-0025-0090 [pii] AID - 10.1159/000486842 [doi] PST - ppublish SO - Ann Neurosci. 2018 Jul;25(2):90-97. doi: 10.1159/000486842. Epub 2018 Feb 2.