PMID- 30141876
OWN - NLM
STAT- MEDLINE
DCOM- 20180907
LR  - 20181202
IS  - 1000-7423 (Print)
IS  - 1000-7423 (Linking)
VI  - 34
IP  - 6
DP  - 2016 Dec
TI  - [Differential Expression of Six wnt Gene Family Members in Echinococcus 
      granulosus Protoscoleces and Adult Worms].
PG  - 552-7
AB  - OBJECTIVE: To investigate the differential mRNA expression and tissue 
      distribution of wnt [wingless-type mouse mammary tumor virus (MMTV) integration 
      site family, wnt] gene members wnt1, wnt2, wnt4, wnt5, wnt11A and wnt11B in 
      protoscoleces and adult worms of Echinococcus granulosus. METHODS: The mRNA 
      expression of wnt1, wnt2, wnt4, wnt5, wnt11A and wnt11B was determined by 
      qRT-PCR. Tissue distribution of wnt1, wnt2, wnt4, wnt5, wnt11A and wnt11B in 
      Echinococcus granulosus protoscoleces was determined by the whole-mount in situ 
      hybridization. RESULTS: The qRT-PCR results showed that the mRNA expression 
      levels of wnt1 and wnt2 in the adult worms were 1.49 （P>0.05） and 2.53 
      folds（P<0.05） of those in the protoscoleces, respectively. The mRNA expression 
      levels of wnt4, wnt5, wnt11A and wnt11B in the protoscoleces were 25.00（P<0.01）, 
      33.33（P<0.01）, 14.29（P<0.01） and 1.03 folds（P>0.05） of those in the adult worms, 
      respectively. In brief, there was no significant difference of mRNA expression in 
      wnt2 and wnt11B between protoscoleces and adult, but there was a significant 
      difference of mRNA expression in wnt1, wnt4, wnt5 and wnt11A between 
      protoscoleces and adults. Results of the whole-mount in situ hybridization showed 
      that in protoscoleces wnt1 was mainly localized in the epidermal tissue, wnt2 in 
      suckers, wnt4 in suckers and rostellum, wnt5 and wnt11B in suckers and epidermal 
      tissue, and wnt11A in rostellum and hooks. CONCLUSION: The mRNA expression of 
      wnt2 in adult E. granulosus was higher than that in protoscoleces, and the mRNA 
      expression ofwnt4, wnt5, wnt11A and wnt11B in protoscoleces was higher than that 
      in the adult worms. The six wnt gene family members were all distributed in the 
      forward region of protoscoleces.
FAU - Wang, Zheng-rong
AU  - Wang ZR
FAU - Zhang, Yan-yan
AU  - Zhang YY
FAU - Bo, Xin-wen
AU  - Bo XW
FAU - Xu, Xue-ping
AU  - Xu XP
FAU - Xu, Chun-sheng
AU  - Xu CS
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
JT  - Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of 
      parasitology & parasitic diseases
JID - 8709992
RN  - 0 (Wnt Proteins)
SB  - IM
MH  - Aging
MH  - Animals
MH  - *Echinococcus granulosus
MH  - In Situ Hybridization
MH  - Mice
MH  - Wnt Proteins
EDAT- 2016/12/01 00:00
MHDA- 2018/09/08 06:00
CRDT- 2018/08/25 06:00
PHST- 2018/08/25 06:00 [entrez]
PHST- 2016/12/01 00:00 [pubmed]
PHST- 2018/09/08 06:00 [medline]
PST - ppublish
SO  - Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2016 Dec;34(6):552-7.