PMID- 30142245 OWN - NLM STAT- MEDLINE DCOM- 20190220 LR - 20190320 IS - 1097-0010 (Electronic) IS - 0022-5142 (Linking) VI - 99 IP - 4 DP - 2019 Mar 15 TI - Changes in the sucrose metabolism in apple fruit following postharvest acibenzolar-S-methyl treatment. PG - 1519-1524 LID - 10.1002/jsfa.9326 [doi] AB - BACKGROUND: Apple (cv. Ralls) fruit were treated with 0.1 g L(-1) acibenzolar-S-methyl (ASM) for 10 min to evaluate the changes in enzyme activity and gene expression in the sucrose metabolism during storage at 20 degrees C with 30%-40% relative humidity. RESULTS: The results showed that sucrose phosphate synthase (SPS) and sucrose synthase synthesis (SS-s) activity was enhanced by ASM in apple fruit during the entire storage period. Sucrose synthase-cleavage (SS-c) and neutral invertase (NI) activity was suppressed by ASM treatment but acid invertase (AI) activity was increased in the middle period after ASM treatment. Acibenzolar-S-methyl treatment also significantly inhibited SPS and NI gene expression in apple fruit during storage. However, SS gene expression increased in the ASM-treated apple fruit. High levels of expression of the fructokinase (FK) and hexokinase (HK) genes were observed during the middle storage period in the ASM-treated fruit. CONCLUSION: Taken together, these results suggest that ASM delays the senescence of apple fruit by regulating the sugar metabolism. (c) 2018 Society of Chemical Industry. CI - (c) 2018 Society of Chemical Industry. FAU - Ge, Yonghong AU - Ge Y AUID- ORCID: 0000-0003-2159-9377 AD - Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Technology, Bohai University, Jinzhou, China. AD - College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin, China. FAU - Wei, Meilin AU - Wei M AD - Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Technology, Bohai University, Jinzhou, China. FAU - Li, Canying AU - Li C AD - Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Technology, Bohai University, Jinzhou, China. FAU - Chen, Yanru AU - Chen Y AUID- ORCID: 0000-0002-6531-7995 AD - Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Technology, Bohai University, Jinzhou, China. FAU - Duan, Bin AU - Duan B AD - Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Technology, Bohai University, Jinzhou, China. FAU - Li, Xue AU - Li X AD - Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Technology, Bohai University, Jinzhou, China. FAU - Tang, Qi AU - Tang Q AD - Food Safety Key Lab of Liaoning Province, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, College of Food Science and Technology, Bohai University, Jinzhou, China. FAU - Li, Xihong AU - Li X AD - College of Food Engineering and Biotechnology, Tianjin University of Science & Technology, Tianjin, China. LA - eng GR - 2017205002/Liaoning Key Research and Development Program/ GR - 2018M630277/China Postdoctoral Science Foundation/ PT - Journal Article DEP - 20181019 PL - England TA - J Sci Food Agric JT - Journal of the science of food and agriculture JID - 0376334 RN - 0 (Food Preservatives) RN - 0 (Plant Proteins) RN - 0 (Thiadiazoles) RN - 57-50-1 (Sucrose) RN - BCW6119347 (S-methyl benzo(1,2,3)thiadiazole-7-carbothioate) RN - EC 2.4.1.- (Glucosyltransferases) RN - EC 2.4.1.13 (sucrose synthase) RN - EC 2.4.1.14 (sucrose-phosphate synthase) SB - IM MH - Food Preservatives/*pharmacology MH - Fruit/*drug effects/enzymology/growth & development/metabolism MH - Gene Expression Regulation, Plant/drug effects MH - Glucosyltransferases/genetics/metabolism MH - Malus/drug effects/enzymology/growth & development/*metabolism MH - Plant Proteins/genetics/metabolism MH - Sucrose/*metabolism MH - Thiadiazoles/*pharmacology OTO - NOTNLM OT - Malus domestica OT - acibenzolar-S-methyl OT - sucrose phosphate synthase, sucrose synthase EDAT- 2018/08/25 06:00 MHDA- 2019/03/21 06:00 CRDT- 2018/08/25 06:00 PHST- 2018/07/06 00:00 [received] PHST- 2018/08/12 00:00 [revised] PHST- 2018/08/19 00:00 [accepted] PHST- 2018/08/25 06:00 [pubmed] PHST- 2019/03/21 06:00 [medline] PHST- 2018/08/25 06:00 [entrez] AID - 10.1002/jsfa.9326 [doi] PST - ppublish SO - J Sci Food Agric. 2019 Mar 15;99(4):1519-1524. doi: 10.1002/jsfa.9326. Epub 2018 Oct 19.