PMID- 30143733
OWN - NLM
STAT- MEDLINE
DCOM- 20191030
LR  - 20230928
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 8
IP  - 1
DP  - 2018 Aug 24
TI  - Optimizing tissue-clearing conditions based on analysis of the critical factors 
      affecting tissue-clearing procedures.
PG  - 12815
LID - 10.1038/s41598-018-31153-7 [doi]
LID - 12815
AB  - Tissue-clearing techniques have received great attention for volume imaging and 
      for the potential to be applied in optical diagnosis. In principle, tissue 
      clearing is achieved by reducing light scattering through a combination of lipid 
      removal, size change, and matching of the refractive index (RI) between the 
      imaging solution and the tissue. However, the contributions of these major 
      factors in tissue clearing have not been systematically evaluated yet. In this 
      study, we experimentally measured and mathematically calculated the contribution 
      of these factors to the clearing of four organs (brain, liver, kidney, and lung). 
      We found that these factors differentially influence the maximal clearing 
      efficacy of tissues and the diffusivity of materials inside the tissue. We 
      propose that these physical properties of organs can be utilized for the quality 
      control (Q/C) process during tissue clearing, as well as for the monitoring of 
      the pathological changes of tissues.
FAU - Kim, June Hoan
AU  - Kim JH
AUID- ORCID: 0000-0001-9078-6892
AD  - Department of Anatomy, Korea University College of Medicine, Seoul, 02841, 
      Republic of Korea.
FAU - Jang, Min Jee
AU  - Jang MJ
AD  - Department of Anatomy, Korea University College of Medicine, Seoul, 02841, 
      Republic of Korea.
FAU - Choi, Jungyoon
AU  - Choi J
AD  - Department of Anatomy, Korea University College of Medicine, Seoul, 02841, 
      Republic of Korea.
FAU - Lee, Eunsoo
AU  - Lee E
AD  - Department of Anatomy, Korea University College of Medicine, Seoul, 02841, 
      Republic of Korea.
FAU - Song, Kyung-Deok
AU  - Song KD
AD  - Department of Physics, Korea University, Seoul, 02841, Republic of Korea.
AD  - Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science 
      (IBS), Seoul, 02841, Republic of Korea.
FAU - Cho, Jaeho
AU  - Cho J
AUID- ORCID: 0000-0001-9966-5157
AD  - Department of Radiation Oncology, Yonsei University College of Medicine, 50 
      Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Republic of Korea.
FAU - Kim, Keun-Tae
AU  - Kim KT
AD  - Department of Life Science, Sogang University, 35th Baekbum-ro Mapo-gu, Seoul, 
      04107, Republic of Korea.
FAU - Cha, Hyuk-Jin
AU  - Cha HJ
AD  - College of Pharmacy, Department of Pharmacy, Seoul National University, 
      Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
FAU - Sun, Woong
AU  - Sun W
AUID- ORCID: 0000-0003-1792-4894
AD  - Department of Anatomy, Korea University College of Medicine, Seoul, 02841, 
      Republic of Korea. woongsun@korea.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180824
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Collagen Type IV)
RN  - 0 (Lipids)
SB  - IM
MH  - Animals
MH  - Collagen Type IV/metabolism
MH  - Diffusion
MH  - Extracellular Matrix/metabolism
MH  - Lipids/isolation & purification
MH  - Mice, Inbred C57BL
MH  - Optical Imaging/*methods
MH  - Refractometry
PMC - PMC6109102
COIS- The authors declare no competing interests.
EDAT- 2018/08/26 06:00
MHDA- 2019/10/31 06:00
PMCR- 2018/08/24
CRDT- 2018/08/26 06:00
PHST- 2018/04/26 00:00 [received]
PHST- 2018/08/13 00:00 [accepted]
PHST- 2018/08/26 06:00 [entrez]
PHST- 2018/08/26 06:00 [pubmed]
PHST- 2019/10/31 06:00 [medline]
PHST- 2018/08/24 00:00 [pmc-release]
AID - 10.1038/s41598-018-31153-7 [pii]
AID - 31153 [pii]
AID - 10.1038/s41598-018-31153-7 [doi]
PST - epublish
SO  - Sci Rep. 2018 Aug 24;8(1):12815. doi: 10.1038/s41598-018-31153-7.