PMID- 30143913
OWN - NLM
STAT- MEDLINE
DCOM- 20190826
LR  - 20200225
IS  - 1568-5608 (Electronic)
IS  - 0925-4692 (Linking)
VI  - 27
IP  - 2
DP  - 2019 Apr
TI  - Melatonin ameliorates TNBS-induced colitis in rats through the melatonin 
      receptors: involvement of TLR4/MyD88/NF-kappaB signalling pathway.
PG  - 361-371
LID - 10.1007/s10787-018-0523-8 [doi]
AB  - AIM: The aim of the present study is to investigate the anti-inflammatory effect 
      of melatonin in trinitrobenzene sulfonic acid (TNBS)-induced rat colitis through 
      the inhibition of Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NF-kappaB) 
      signalling pathway and activation of melatonin receptor. METHODS: Colitis was 
      induced in Wistar rats by administration of 100 mg/kg TNBS dissolved in 0.25 ml 
      of 50% ethanol solution using a flexible plastic rubber catheter into the colon 
      via the anus. This resulted in incidence of colitis on the first day, and all 
      treatments were conducted for 10 days after induction of colitis. Melatonin was 
      administered intraperitoneally (i.p.) at doses of 1, 5, and 10 mg/kg/day. 
      Luzindole (non-selective MT1/MT2 receptor antagonist) was administered i.p. at 
      dose of 5 mg/kg/day 15 min prior to melatonin injection. During the experiment, 
      animals were monitored for the appearance of diarrhoea, body weight loss, and 
      rectal bleeding. Myeloid peroxidase enzyme and tumour necrosis factor-alpha (TNF-alpha) 
      activities were detected by immunohistochemistry. The protein expression level of 
      TLR4, myeloid differentiation factor 88 (MyD88), NF-kappaB p65, and inhibitor of 
      kappa B (I-kappaB) were detected by western blotting analysis. RESULTS: Treatment 
      with melatonin improved weight loss, mucosal, and histological damage compared 
      with TNBS group. In addition, melatonin decreased TNBS-induced up-regulation of 
      TLR4, MyD88, and NF-kappaB p65, and increased down-regulation of I-kappaB proteins. On 
      the other hand, the administration of luzindole resulted in the inhibition of 
      melatonin effects. CONCLUSIONS: It seems that the inhibition of TLR4/NF-kappaB 
      signalling pathway may mediate the anti-inflammatory effects of melatonin in 
      TNBS-induced rat colitis.
FAU - Chamanara, Mohsen
AU  - Chamanara M
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, P.O. Box 13145-784, Tehran, Iran.
FAU - Rashidian, Amir
AU  - Rashidian A
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, P.O. Box 13145-784, Tehran, Iran.
AD  - Experimental Medicine Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Mehr, Shahram Ejtemaei
AU  - Mehr SE
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, P.O. Box 13145-784, Tehran, Iran.
FAU - Dehpour, Ahmad-Reza
AU  - Dehpour AR
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, P.O. Box 13145-784, Tehran, Iran.
AD  - Experimental Medicine Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Shirkohi, Reza
AU  - Shirkohi R
AD  - Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Akbarian, Reyhaneh
AU  - Akbarian R
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, P.O. Box 13145-784, Tehran, Iran.
FAU - Abdollahi, Alireza
AU  - Abdollahi A
AD  - Department of Pathology, Imam Hospital, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Rezayat, Seyed-Mahdi
AU  - Rezayat SM
AD  - Department of Pharmacology, School of Medicine, Tehran University of Medical 
      Sciences, P.O. Box 13145-784, Tehran, Iran. Rezayat@tums.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20180824
PL  - Switzerland
TA  - Inflammopharmacology
JT  - Inflammopharmacology
JID - 9112626
RN  - 0 (Myd88 protein, rat)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Melatonin)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
RN  - JL5DK93RCL (Melatonin)
MH  - Animals
MH  - Colitis/chemically induced/*drug therapy/metabolism
MH  - Colon/drug effects/metabolism
MH  - Down-Regulation/drug effects
MH  - Intestinal Mucosa/drug effects/metabolism
MH  - Male
MH  - Melatonin/*pharmacology
MH  - Myeloid Differentiation Factor 88/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Melatonin/*metabolism
MH  - Signal Transduction/*drug effects
MH  - Toll-Like Receptor 4/*metabolism
MH  - Trinitrobenzenesulfonic Acid/pharmacology
MH  - Up-Regulation/drug effects
OTO - NOTNLM
OT  - Melatonin
OT  - TLR4/NF-kappaB signalling pathway
OT  - TNBS
OT  - Ulcerative colitis
EDAT- 2018/08/26 06:00
MHDA- 2019/08/27 06:00
CRDT- 2018/08/26 06:00
PHST- 2017/11/15 00:00 [received]
PHST- 2018/02/07 00:00 [accepted]
PHST- 2018/08/26 06:00 [pubmed]
PHST- 2019/08/27 06:00 [medline]
PHST- 2018/08/26 06:00 [entrez]
AID - 10.1007/s10787-018-0523-8 [pii]
AID - 10.1007/s10787-018-0523-8 [doi]
PST - ppublish
SO  - Inflammopharmacology. 2019 Apr;27(2):361-371. doi: 10.1007/s10787-018-0523-8. 
      Epub 2018 Aug 24.