PMID- 30144033
OWN - NLM
STAT- MEDLINE
DCOM- 20200102
LR  - 20200102
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 234
IP  - 3
DP  - 2019 Mar
TI  - Human umbilical cord tissue stem cells and neuronal lineages in an injectable 
      caffeic acid-bioconjugated gelatin hydrogel for transplantation.
PG  - 1967-1977
LID - 10.1002/jcp.26834 [doi]
AB  - Present-day scaffolds are useful in cell therapy to a reasonable extent, but in 
      pursuit of improvising the scaffold to improve the outcome, we tested a new 
      injectable caffeic acid-bioconjugated gelatin hydrogel scaffold (CBGH; with 
      tunable stiffness -10%). Two-dimensional (2D) form of human umbilical cord 
      tissue-derived mesenchymal stem cells (HUCMSCs) culture performed based on our 
      previously reported methods and characterized by using multipotent and 
      pluripotent analysis. In addition, neurogenesis was induced in the presence of 
      retinoic acid or neural growth factor or epidermal growth factor categorized by 
      neuronal markers. The viability, proliferation rate, and vascular endothelial 
      growth factor expression of HUCMSCs increased significantly in the CBGH scaffold. 
      In addition, there was an increase in CD90 and TRA-1-81 phenotypic expressions 
      and SOX-2, MAP-2, TAU, NeuN, and NF, which confirmed the neurogenesis of 
      encapsulated HUCMSCs. Topographical elucidation by scanning electron microscopy 
      data showed that the HUCMSCs proliferated and migrated inside the construct. 
      Hematoxylin and eosin staining demonstrated a more viable structural pattern and 
      cresyl violet staining showed the Nissl synthesis, confirming the presence of 
      functional neurons in the encapsulated form. The molecular-level analysis further 
      substantiated that HUCMSCs cultured in CBGH expressed significantly greater 
      upregulation of stemness, neuronal genes, and protein expression compared with 
      the adherent culture. Correspondingly, this is the first time that we have 
      measured the fluorescence intensity variation of the HUCMSCs-stained cell 
      segmentation process using customized MATLAB code execution to reduce the 
      background noise and autofluorescence. We conclude that this novel CBGH scaffold 
      increases the viability, proliferation, stemness, and also neuronal 
      transdifferentiation of HUCMSCs in a three-dimensional culture than the 2D 
      plastic adherent culture.
CI  - (c) 2018 Wiley Periodicals, Inc.
FAU - Subbarayan, Rajasekaran
AU  - Subbarayan R
AD  - Centre for Regenerative Medicine and Stem Cell Research, Central Research 
      Facility, Sri Ramachandra Medical College and Research Institute, Porur, India.
FAU - Girija, Dinesh Murugan
AU  - Girija DM
AUID- ORCID: 0000-0002-1277-7787
AD  - Centre for Indian Systems of Medicine Quality Assurance and Standardization, 
      Central Research Facility, Sri Ramachandra Medical College and Research 
      Institute, Porur, India.
FAU - Rao, Suresh Ranga
AU  - Rao SR
AUID- ORCID: 0000-0001-9247-6695
AD  - Department of Periodontology and Implantology, Faculty of Dental Sciences, Centre 
      for Regenerative Medicine and Stem Cell Research, Sri Ramachandra Medical College 
      and Research Institute, Porur, India.
LA  - eng
PT  - Journal Article
DEP - 20180824
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Biomarkers)
RN  - 0 (Caffeic Acids)
RN  - 0 (Hydrogels)
RN  - 9000-70-8 (Gelatin)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Caffeic Acids
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Gelatin
MH  - Hereditary Sensory and Autonomic Neuropathies
MH  - Humans
MH  - Hydrogels
MH  - Injections
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Microscopy, Electron, Scanning
MH  - Neural Stem Cells/*cytology/metabolism/*transplantation
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/cytology
OTO - NOTNLM
OT  - caffeic acid-gelatin hydrogel
OT  - multipotency
OT  - neuronal lineage
OT  - pluripotency
OT  - umbilical cord mesenchymal stem cells
EDAT- 2018/08/26 06:00
MHDA- 2020/01/03 06:00
CRDT- 2018/08/26 06:00
PHST- 2018/01/31 00:00 [received]
PHST- 2018/05/10 00:00 [accepted]
PHST- 2018/08/26 06:00 [pubmed]
PHST- 2020/01/03 06:00 [medline]
PHST- 2018/08/26 06:00 [entrez]
AID - 10.1002/jcp.26834 [doi]
PST - ppublish
SO  - J Cell Physiol. 2019 Mar;234(3):1967-1977. doi: 10.1002/jcp.26834. Epub 2018 Aug 
      24.