PMID- 30144351
OWN - NLM
STAT- MEDLINE
DCOM- 20190725
LR  - 20190725
IS  - 1744-7909 (Electronic)
IS  - 1672-9072 (Linking)
VI  - 61
IP  - 4
DP  - 2019 Apr
TI  - Rice miR394 suppresses leaf inclination through targeting an F-box gene, LEAF 
      INCLINATION 4.
PG  - 406-416
LID - 10.1111/jipb.12713 [doi]
AB  - Rice leaf inclination is an important agronomic trait, closely related to plant 
      architecture and yield. Identification of genes controlling leaf inclination 
      would assist in crop improvement. Although various factors, including the plant 
      hormones auxin and brassinosteroids, have been shown to regulate lamina joint 
      development, the role of microRNAs in regulating leaf inclination remains largely 
      unknown. Here, we functionally characterize the role of rice miR394 and its 
      target, LEAF INCLINCATION 4 (LC4), which encodes an F-box protein, in the 
      regulation of leaf inclination. We show that miR394 and LC4 work, 
      antagonistically, to regulate leaf lamina joint development and rice 
      architecture, by modulating expansion and elongation of adaxial parenchyma cells. 
      Suppressed expression of miR394, or enhanced expression of LC4, results in 
      enlarged leaf angles, whereas reducing LC4 expression by CRISPR/Cas9 leads to 
      reduced leaf inclination, suggesting LC4 as candidate for use in rice 
      architecture improvement. LC4 interacts with SKP1, a component of the SCF E3 
      ubiquitin ligase complex, and transcription of both miR394 and LC4 are regulated 
      by auxin. Rice plants with altered expression of miR394 or LC4 have altered auxin 
      responses, indicating that the miR394-LC4 module mediates auxin effects important 
      for determining rice leaf inclination and architecture.
CI  - (c) 2018 Institute of Botany, Chinese Academy of Sciences.
FAU - Qu, Li
AU  - Qu L
AD  - National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in 
      Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, the 
      Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.
AD  - University of the Chinese Academy of Sciences, Beijing 100049, China.
FAU - Lin, Li-Bi
AU  - Lin LB
AD  - National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in 
      Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, the 
      Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.
AD  - University of the Chinese Academy of Sciences, Beijing 100049, China.
FAU - Xue, Hong-Wei
AU  - Xue HW
AD  - National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in 
      Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, the 
      Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.
LA  - eng
GR  - 91535201/National Nature Science Foundation of China/
GR  - State Key Laboratory of Wheat and Maize Crop Science/
GR  - Southern Regional Collaborative Innovation Center for Grain and Oil Crops in 
      China/
PT  - Journal Article
DEP - 20181112
PL  - China (Republic : 1949- )
TA  - J Integr Plant Biol
JT  - Journal of integrative plant biology
JID - 101250502
RN  - 0 (Brassinosteroids)
RN  - 0 (Indoleacetic Acids)
RN  - 0 (MicroRNAs)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Messenger)
MH  - Base Sequence
MH  - Brassinosteroids/metabolism
MH  - Gene Expression Regulation, Plant/drug effects
MH  - *Genes, Plant
MH  - Indoleacetic Acids/pharmacology
MH  - MicroRNAs/*genetics/metabolism
MH  - Oryza/*genetics
MH  - Plant Leaves/drug effects/*genetics
MH  - Plant Proteins/*genetics/metabolism
MH  - Protein Binding/drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Signal Transduction/drug effects
MH  - Transcription, Genetic
EDAT- 2018/08/26 06:00
MHDA- 2019/07/26 06:00
CRDT- 2018/08/26 06:00
PHST- 2018/05/27 00:00 [received]
PHST- 2018/08/24 00:00 [accepted]
PHST- 2018/08/26 06:00 [pubmed]
PHST- 2019/07/26 06:00 [medline]
PHST- 2018/08/26 06:00 [entrez]
AID - 10.1111/jipb.12713 [doi]
PST - ppublish
SO  - J Integr Plant Biol. 2019 Apr;61(4):406-416. doi: 10.1111/jipb.12713. Epub 2018 
      Nov 12.