PMID- 30145454
OWN - NLM
STAT- MEDLINE
DCOM- 20190110
LR  - 20211204
IS  - 1873-264X (Electronic)
IS  - 0731-7085 (Linking)
VI  - 161
DP  - 2018 Nov 30
TI  - A rapid, simple, and high-throughput UPLC-MS/MS method for simultaneous 
      determination of bioactive constituents in Salvia miltiorrhiza with 
      positive/negative ionization switching.
PG  - 94-100
LID - S0731-7085(18)31491-2 [pii]
LID - 10.1016/j.jpba.2018.08.027 [doi]
AB  - The dried root of Danshen (Salvia miltiorrhiza) is a Chinese medicine that has 
      been used in traditional treatments for more than 2000 years in Asia. It has also 
      been used to treat cardiovascular diseases for decades. The goal of this study 
      was to develop a new, fast, and simple ultrahigh-performance liquid 
      chromatography tandem mass spectrometry (UPLC-MS/MS) analytical method for the 
      analysis of phenolic acids and tanshinones in Danshen. The samples were separated 
      on a Kinetex F5 column (2.1 x 100 mm, 1.7 mum, pentafluorophenyl) using 78% 
      acetonitrile with 0.1% formic acid as the mobile phase with isocratic elution 
      while the flow rate was set at 0.45 mL/min. The analysis was performed in a 
      Waters Xevo TQ mass spectrometer via multiple reaction monitoring (MRM) in 
      polarity switching mode. The linearity values of the calibration curves of eight 
      compounds were in the range from 3 to 3200 ng/mL (r(2) > 0.99). Method validation 
      was performed in terms of linearity, the relative standard deviations (RSDs) of 
      the intra-day and inter-day, and it was found that precisions were within 15% and 
      the accuracy ranged from 93% to 118%. The recovery was more than 87%. The limit 
      of detection (LOD) and limit of quantification (LOQ) values of the eight analytes 
      varied between 0.023 to 0.75 ng/mL and 0.375 to 1.5 ng/mL, respectively. This 
      approach has the shortest analysis time for the separation of phenolic acids and 
      tanshinones in Danshen using a UPLC core-shell column with F5 stationary phase. 
      Meanwhile, this robust and high-throughput method not only has a short run time 
      (2 min) but also reduce the consumption of organic solvents.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Lin, Hung-Yu
AU  - Lin HY
AD  - Institute of Molecular Biology, National Chung Hsing University, Taichung 40227, 
      Taiwan.
FAU - Lin, Ting-Sian
AU  - Lin TS
AD  - Institute of Molecular Biology, National Chung Hsing University, Taichung 40227, 
      Taiwan.
FAU - Chien, Han-Ju
AU  - Chien HJ
AD  - Institute of Molecular Biology, National Chung Hsing University, Taichung 40227, 
      Taiwan.
FAU - Juang, Yu-Min
AU  - Juang YM
AD  - Waters Corporation, No. 90 Jianguo North Road, Zhongshan District, Taipei City 
      10491, Taiwan.
FAU - Chen, Chao-Jung
AU  - Chen CJ
AD  - Proteomics Core Lab, Department of Medical Research, China Medical University 
      Hospital, Taichung 40402, Taiwan; Graduate Institute of Integrated Medicine, 
      China Medical University, Taichung 40402, Taiwan.
FAU - Wang, Chang-Sheng
AU  - Wang CS
AD  - Department of Agronomy, National Chung Hsing University, Taichung 40227, Taiwan; 
      Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung 
      40402, Taiwan. Electronic address: wangchansen@nchu.edu.tw.
FAU - Lai, Chien-Chen
AU  - Lai CC
AD  - Institute of Molecular Biology, National Chung Hsing University, Taichung 40227, 
      Taiwan; Advanced Plant Biotechnology Center, National Chung Hsing University, 
      Taichung 40402, Taiwan; Graduate institute of Chinese Medical Science, China 
      Medical University, Taichung 40402, Taiwan. Electronic address: 
      lailai@dragon.nchu.edu.tw.
LA  - eng
PT  - Journal Article
DEP - 20180816
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Abietanes)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Hydroxybenzoates)
RN  - 03UUH3J385 (tanshinone)
RN  - 1693AM5SBN (dan-shen root extract)
RN  - I3P9R8317T (phenolic acid)
SB  - IM
MH  - Abietanes/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drugs, Chinese Herbal/*chemistry
MH  - High-Throughput Screening Assays/methods
MH  - Hydroxybenzoates/*analysis
MH  - Limit of Detection
MH  - Salvia miltiorrhiza/*chemistry
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Phenolic acids
OT  - Salvia miltiorrhiza
OT  - Tanshinones
OT  - UPLC-MS/MS
EDAT- 2018/08/27 06:00
MHDA- 2019/01/11 06:00
CRDT- 2018/08/27 06:00
PHST- 2018/06/23 00:00 [received]
PHST- 2018/08/11 00:00 [revised]
PHST- 2018/08/12 00:00 [accepted]
PHST- 2018/08/27 06:00 [pubmed]
PHST- 2019/01/11 06:00 [medline]
PHST- 2018/08/27 06:00 [entrez]
AID - S0731-7085(18)31491-2 [pii]
AID - 10.1016/j.jpba.2018.08.027 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2018 Nov 30;161:94-100. doi: 10.1016/j.jpba.2018.08.027. 
      Epub 2018 Aug 16.