PMID- 30146262
OWN - NLM
STAT- MEDLINE
DCOM- 20190108
LR  - 20211204
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 503
IP  - 4
DP  - 2018 Sep 18
TI  - Selenoprotein S protects against adipocyte death through mediation of the 
      IRE1alpha-sXBP1 pathway.
PG  - 2866-2871
LID - S0006-291X(18)31739-X [pii]
LID - 10.1016/j.bbrc.2018.08.057 [doi]
AB  - As the most conserved branch of the unfolded protein response (UPR), the 
      inositol-requiring enzyme 1a (IRE1a)/X-box binding protein 1 (XBP1) pathway plays 
      crucial roles in cell survival and cell death by upregulating UPR-associated 
      genes involved in protein entry into the endoplasmic reticulum (ER) and 
      ER-associated degradation (ERAD). Selenoprotein S (SelS) is localized to the ER 
      membrane and involved in ERAD. Although SelS plays an important role in restoring 
      ER stress, the SelS-dependent protective mechanisms against cell death remain 
      unclear. Here, using an inducible SelS knockdown (KD) 3T3-L1 cell model, we 
      showed that SelS KD resulted adipocyte death, which was associated with imbalance 
      of the Bcl-2 family members. Furthermore, SelS KD decreased spliced XBP1 (sXBP1), 
      increased IRE1alpha and p-JNK, suggesting a role of SelS in the modulation of the 
      IRE1alpha-sXBP1 pathway. Moreover, adipocyte death induced by SelS suppression can be 
      inhibited by overexpression of sXBP1. Thus, it is proposed that SelS promotes 
      cell survival through the IRE1alpha-XBP1 signaling pathway.
CI  - Copyright (c) 2018. Published by Elsevier Inc.
FAU - Men, Lili
AU  - Men L
AD  - Department of Endocrinology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Yu, Shanshan
AU  - Yu S
AD  - Department of Endocrinology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Yao, Junjie
AU  - Yao J
AD  - Department of Endocrinology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Li, Yu
AU  - Li Y
AD  - Department of Endocrinology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Ren, Decheng
AU  - Ren D
AD  - Department of Medicine, The University of Chicago, Chicago, IL, USA. Electronic 
      address: decheng@uchicago.edu.
FAU - Du, Jianling
AU  - Du J
AD  - Department of Endocrinology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China. Electronic address: dujianling63@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180824
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Selenoproteins)
RN  - 0 (X-Box Binding Protein 1)
RN  - 0 (Xbp1 protein, mouse)
RN  - EC 2.7.11.1 (Ern1 protein, mouse)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EC 3.1.- (Endoribonucleases)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/cytology/*metabolism
MH  - Animals
MH  - Cell Death/*genetics
MH  - Cell Differentiation
MH  - Endoplasmic Reticulum/metabolism
MH  - Endoplasmic Reticulum Stress/genetics
MH  - *Endoplasmic Reticulum-Associated Degradation
MH  - Endoribonucleases/*genetics/metabolism
MH  - Gene Expression Regulation
MH  - MAP Kinase Kinase 4/genetics/metabolism
MH  - Mice
MH  - Protein Serine-Threonine Kinases/*genetics/metabolism
MH  - Protein Transport
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Selenoproteins/antagonists & inhibitors/*genetics/metabolism
MH  - Signal Transduction
MH  - X-Box Binding Protein 1/*genetics/metabolism
OTO - NOTNLM
OT  - Cell death
OT  - IRE1alpha
OT  - SelS
OT  - Spliced XBP1
EDAT- 2018/08/28 06:00
MHDA- 2019/01/09 06:00
CRDT- 2018/08/28 06:00
PHST- 2018/08/05 00:00 [received]
PHST- 2018/08/06 00:00 [accepted]
PHST- 2018/08/28 06:00 [pubmed]
PHST- 2019/01/09 06:00 [medline]
PHST- 2018/08/28 06:00 [entrez]
AID - S0006-291X(18)31739-X [pii]
AID - 10.1016/j.bbrc.2018.08.057 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2018 Sep 18;503(4):2866-2871. doi: 
      10.1016/j.bbrc.2018.08.057. Epub 2018 Aug 24.