PMID- 30146756 OWN - NLM STAT- MEDLINE DCOM- 20191206 LR - 20221020 IS - 1097-4652 (Electronic) IS - 0021-9541 (Linking) VI - 234 IP - 2 DP - 2019 Feb TI - Long non-coding RNA LINC00152 promotes cell growth and invasion of papillary thyroid carcinoma by regulating the miR-497/BDNF axis. PG - 1336-1345 LID - 10.1002/jcp.26928 [doi] AB - Long intergenic non-coding RNA 152 (LINC00152) was reported to be tightly linked to tumorigenesis and progression in multiple cancers. However, its biological role and modulatory mechanism in papillary thyroid carcinoma (PTC) has not been elucidated. In this study, we determined the expression levels of LINC00152 in PTC tissues and cell lines by quantitative real time polymerase chain reaction (qRT-PCR). Cell proliferation, colony formation, migration, and invasion were measured by a Cell Counting Kit-8 assay, colony formation analysis, wound healing, and transwell invasion assay, respectively. A luciferase reporter assay and qRT-PCR were used to determine whether LINC00152 interacts with miR-497 directly. We established a xenograft mouse model to examine the underlying molecular mechanism and effect of LINC00152 on tumor growth in vivo. We found that LINC00152 expression was significantly increased in PTC tissues and derived cell lines. LINC00152 knockdown significantly inhibited proliferation, colony formation, migration, and invasion in vitro, and impaired tumor growth in vivo. We revealed that LINC00152 functioned as a competing endogenous RNA to the miR-497 sponge, downregulating its downstream target brain-derived neurotrophic factor (BDNF), which is an oncogene in thyroid cancer. These findings suggest that LINC00152 is responsible for PTC cell proliferation and invasion and exerts its function by regulating the miR-497/BDNF axis. CI - (c) 2018 Wiley Periodicals, Inc. FAU - Sun, Zhihui AU - Sun Z AD - Department of Drug Management, The First Hospital of Jilin University, Changchun, Jilin, China. FAU - Guo, Xun AU - Guo X AD - Department of Hyperbaric Oxygen Therapy, The First Hospital of Jilin University, Changchun, Jilin, China. FAU - Zang, Mingcui AU - Zang M AD - Department of Hepatobiliary pancreatic surgery, The First Hospital of Jilin University, Changchun, Jilin, China. FAU - Wang, Peisong AU - Wang P AUID- ORCID: 0000-0003-3222-938X AD - Department of Thyroid surgery, The First Hospital of Jilin University, Changchun, Jilin, China. FAU - Xue, Shuai AU - Xue S AD - Department of Thyroid surgery, The First Hospital of Jilin University, Changchun, Jilin, China. FAU - Chen, Guang AU - Chen G AD - Department of Thyroid surgery, The First Hospital of Jilin University, Changchun, Jilin, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Retracted Publication DEP - 20180826 PL - United States TA - J Cell Physiol JT - Journal of cellular physiology JID - 0050222 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (MIRN497 microRNA, human) RN - 0 (MicroRNAs) RN - 0 (RNA, Long Noncoding) RN - 0 (long non-coding RNA Linc00152, human) RN - 7171WSG8A2 (BDNF protein, human) SB - IM RIN - J Cell Physiol. 2022 Nov;237(11):4327. PMID: 36264182 MH - Animals MH - Brain-Derived Neurotrophic Factor/genetics/*metabolism MH - Cell Line, Tumor MH - *Cell Movement MH - *Cell Proliferation MH - Female MH - Gene Expression Regulation, Neoplastic MH - HEK293 Cells MH - Humans MH - Mice, Inbred BALB C MH - Mice, Nude MH - MicroRNAs/genetics/*metabolism MH - Neoplasm Invasiveness MH - RNA, Long Noncoding/genetics/*metabolism MH - Signal Transduction MH - Thyroid Cancer, Papillary/genetics/*metabolism/pathology MH - Thyroid Neoplasms/genetics/*metabolism/pathology MH - Tumor Burden OTO - NOTNLM OT - LINC00152 OT - brain-derived neurotrophic factor (BDNF) OT - miR-497 OT - papillary thyroid carcinoma (PTC) EDAT- 2018/08/28 06:00 MHDA- 2019/12/18 06:00 CRDT- 2018/08/28 06:00 PHST- 2018/02/20 00:00 [received] PHST- 2018/06/13 00:00 [accepted] PHST- 2018/08/28 06:00 [pubmed] PHST- 2019/12/18 06:00 [medline] PHST- 2018/08/28 06:00 [entrez] AID - 10.1002/jcp.26928 [doi] PST - ppublish SO - J Cell Physiol. 2019 Feb;234(2):1336-1345. doi: 10.1002/jcp.26928. Epub 2018 Aug 26.