PMID- 30149753
OWN - NLM
STAT- MEDLINE
DCOM- 20181218
LR  - 20181218
IS  - 1793-6853 (Electronic)
IS  - 0192-415X (Linking)
VI  - 46
IP  - 6
DP  - 2018
TI  - Ethanol Extract of Lilium Bulbs Plays an Anti-Inflammatory Role by Targeting the 
      IKK[Formula: see text]/[Formula: see text]-Mediated NF-[Formula: see text]B 
      Pathway in Macrophages.
PG  - 1281-1296
LID - 10.1142/S0192415X18500672 [doi]
AB  - Lilium bulbs have long been used as Chinese traditional medicines to alleviate 
      the symptoms of various human inflammatory diseases. However, mechanisms of 
      Lilium bulb-mediated anti-inflammatory activity and the bioactive components in 
      Lilium bulbs remain unknown. In the present study, the anti-inflammatory activity 
      of Lilium bulbs and the underlying mechanism of action were investigated in 
      macrophages using Lilium bulb ethanol extracts (Lb-EE). In a dose-dependent 
      manner, Lb-EE inhibited nitric oxide (NO) production in lipopolysaccharide 
      (LPS)-stimulated RAW264.7 cells and bone marrow-derived macrophages (BMDMs) 
      without causing significant cytotoxicity. Lb-EE also down-regulated mRNA 
      expression of inflammatory genes in LPS-stimulated RAW264.7 cells, which included 
      inducuble nitric oxide synthase (iNOS), cyclooxygenase-2 (COX2), and tumor 
      necrosis factor-[Formula: see text] (TNF-[Formula: see text]). Furthermore, Lb-EE 
      markedly restored LPS-induced morphological changes in RAW264.7 cells to a normal 
      morphology. HPLC analysis identified quercetin, luteolin, and kaempferol as 
      bioactive components contained in Lb-EE. Mechanistic studies in LPS-stimulated 
      RAW264.7 cells revealed that Lb-EE suppressed MyD88- and TRIF-induced 
      NF-[Formula: see text]B transcriptional activation and the nuclear translocation 
      of NF-[Formula: see text]B transcription factors. Moreover, Lb-EE inhibited 
      IKK[Formula: see text]/[Formula: see text]-induced activation of the NF-[Formula: 
      see text]B signaling pathway and IKK inhibition significantly reduced NO 
      production in LPS-stimulated RAW264.7 cells. Taken together, these results 
      suggest that Lb-EE plays an anti-inflammatory role by targeting IKK[Formula: see 
      text]/[Formula: see text]-mediated activation of the NF-[Formula: see text]B 
      signaling pathway during macrophage-mediated inflammatory responses.
FAU - Han, Sang Yun
AU  - Han SY
AD  - * Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, 
      Republic of Korea.
FAU - Yi, Young-Su
AU  - Yi YS
AD  - dagger Department of Pharmaceutical Engineering, Cheongju University, Cheongju 28503, 
      Republic of Korea.
FAU - Jeong, Seong-Gu
AU  - Jeong SG
AD  - * Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, 
      Republic of Korea.
FAU - Hong, Yo Han
AU  - Hong YH
AD  - * Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, 
      Republic of Korea.
FAU - Choi, Kang Jun
AU  - Choi KJ
AD  - double dagger Horticultural Research Division, Gangwondo Provincial Agricultural Research and 
      Extension Services, Chuncheon 24226, Republic of Korea.
FAU - Hossain, Mohammad Amjad
AU  - Hossain MA
AD  -  section sign Department of Veterinary Physiology, College of Medicine, Chonbuk National 
      University, Iksan 54596, Republic of Korea.
FAU - Hwang, Hyunsik
AU  - Hwang H
AD  - * Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, 
      Republic of Korea.
FAU - Rho, Ho Sik
AU  - Rho HS
AD  -  paragraph sign Department of Chemical Engineering, College of Engineering, Suwon University, 
      Suwon 18323, Republic of Korea.
FAU - Lee, Jongsung
AU  - Lee J
AD  - * Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, 
      Republic of Korea.
FAU - Kim, Jong-Hoon
AU  - Kim JH
AD  -  section sign Department of Veterinary Physiology, College of Medicine, Chonbuk National 
      University, Iksan 54596, Republic of Korea.
FAU - Cho, Jae Youl
AU  - Cho JY
AD  - * Department of Genetic Engineering, Sungkyunkwan University, Suwon 16419, 
      Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20180827
PL  - Singapore
TA  - Am J Chin Med
JT  - The American journal of Chinese medicine
JID - 7901431
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 3K9958V90M (Ethanol)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
SB  - IM
MH  - Animals
MH  - *Anti-Inflammatory Agents
MH  - Cell Survival/drug effects
MH  - Ethanol
MH  - HEK293 Cells
MH  - Humans
MH  - I-kappa B Kinase/*metabolism
MH  - Lilium/*chemistry
MH  - Macrophages/*immunology/*metabolism
MH  - Mice
MH  - NF-kappa B/*metabolism
MH  - Nitric Oxide/metabolism
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - RAW 264.7 Cells
MH  - Signal Transduction/*drug effects/*genetics
OTO - NOTNLM
OT  - Anti-Inflammatory
OT  - IKK/
OT  - Lilium Bulbs
OT  - Macrophage
OT  - NF-B
EDAT- 2018/08/29 06:00
MHDA- 2018/12/19 06:00
CRDT- 2018/08/29 06:00
PHST- 2018/08/29 06:00 [pubmed]
PHST- 2018/12/19 06:00 [medline]
PHST- 2018/08/29 06:00 [entrez]
AID - 10.1142/S0192415X18500672 [doi]
PST - ppublish
SO  - Am J Chin Med. 2018;46(6):1281-1296. doi: 10.1142/S0192415X18500672. Epub 2018 
      Aug 27.