PMID- 30151077
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 2001-3078 (Print)
IS  - 2001-3078 (Electronic)
IS  - 2001-3078 (Linking)
VI  - 7
IP  - 1
DP  - 2018
TI  - Microfluidics-based on-a-chip systems for isolating and analysing extracellular 
      vesicles.
PG  - 1508271
LID - 10.1080/20013078.2018.1508271 [doi]
LID - 1508271
AB  - Extracellular vesicles (EVs), which can be found in almost all body fluids, 
      consist of a lipid bilayer enclosing proteins and nucleic acids from their cells 
      of origin. EVs can transport their cargo to target cells and have therefore 
      emerged as key players in intercellular communication. Their potential as either 
      diagnostic and prognostic biomarkers or therapeutic drug delivery systems (DDSs) 
      has generated considerable interest in recent years. However, conventional 
      methods used to study EVs still have significant limitations including the 
      time-consuming and low throughput techniques required, while at the same time the 
      demand for better research tools is getting stronger and stronger. In the past 
      few years, microfluidics-based technologies have gradually emerged and have come 
      to play an essential role in the isolation, detection and analysis of EVs. Such 
      technologies have several advantages, including low cost, low sample volumes, 
      high throughput and precision. This review summarizes recent advances in 
      microfluidics-based technologies, compares conventional and microfluidics-based 
      technologies, and includes a brief survey of recent progress towards integrated 
      "on-a-chip" systems. In addition, this review also discusses the potential 
      clinical applications of "on-a-chip" systems, including both "liquid biopsies" 
      for personalized medicine and DDS devices for precision medicine, and then 
      anticipates the possible future participation of cloud-based portable disease 
      diagnosis and monitoring systems, possibly with the participation of artificial 
      intelligence (AI).
FAU - Guo, Shang-Chun
AU  - Guo SC
AUID- ORCID: 0000-0001-6285-6750
AD  - Institute of Microsurgery on Extremities, Shanghai Jiao Tong University 
      Affiliated Sixth People's Hospital, Shanghai, China.
FAU - Tao, Shi-Cong
AU  - Tao SC
AUID- ORCID: 0000-0002-8706-6138
AD  - Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth 
      People's Hospital, Shanghai, China.
FAU - Dawn, Helen
AU  - Dawn H
AD  - Faculty of Health and Life Science, Oxford Brookes University, Oxford, UK.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20180820
PL  - United States
TA  - J Extracell Vesicles
JT  - Journal of extracellular vesicles
JID - 101610479
PMC - PMC6104604
OTO - NOTNLM
OT  - DDS
OT  - Extracellular vesicle
OT  - RNA
OT  - biomarker
OT  - cell-free therapy
OT  - drug delivery system
OT  - exosomes
OT  - microfluidics
OT  - on-a-chip
OT  - protein
EDAT- 2018/08/29 06:00
MHDA- 2018/08/29 06:01
PMCR- 2018/08/20
CRDT- 2018/08/29 06:00
PHST- 2018/04/02 00:00 [received]
PHST- 2018/06/13 00:00 [revised]
PHST- 2018/07/25 00:00 [accepted]
PHST- 2018/08/29 06:00 [entrez]
PHST- 2018/08/29 06:00 [pubmed]
PHST- 2018/08/29 06:01 [medline]
PHST- 2018/08/20 00:00 [pmc-release]
AID - 1508271 [pii]
AID - 10.1080/20013078.2018.1508271 [doi]
PST - epublish
SO  - J Extracell Vesicles. 2018 Aug 20;7(1):1508271. doi: 
      10.1080/20013078.2018.1508271. eCollection 2018.