PMID- 30153481
OWN - NLM
STAT- MEDLINE
DCOM- 20180924
LR  - 20180924
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 297
DP  - 2018 Nov
TI  - Transgenerational impaired spermatogenesis with sperm H19 and Gtl2 
      hypomethylation induced by the endocrine disruptor p,p'-DDE.
PG  - 34-41
LID - S0378-4274(18)31813-7 [pii]
LID - 10.1016/j.toxlet.2018.08.015 [doi]
AB  - The hypothesis of fetal origins unraveled that environmental exposures in early 
      life could alter epigenetic modifications in the male germ-line, increasing the 
      susceptibility to adult-onset diseases for generations. In our previous study, 
      p,p'-dichlorodiphenoxydichloroethylene (p,p'-DDE), a representative endocrine 
      disruptor, was found to induce transgenerational impaired male fertility with 
      sperm Igf2 hypomethylation. However, whether other imprinted genes involved 
      remains uncovered. In the present study, we administered p,p'-DDE (100 mg/kg body 
      weight) to pregnant Sprague-Dawley rats from gestational day (GD) 8 to GD15. Male 
      F1 offspring were mated with the females from the same group to obtain F2 
      progeny. F3 progeny was obtained by inter-crossing the control and exposed F2 
      generation. Gestational exposure to p,p'-DDE impaired the testis histology and 
      decreased the sperm fertility with H19 and Gtl2 hypomethylation in F1 offspring. 
      Interestingly, this impaired spermatogenesis and DNA hypomethylation were 
      maintained through F2 and F3 generations. DNA methyltransferase DNMT1 and 3a were 
      significantly decreased in the embryonic testis of p,p'-DDE-treated F1 and F2 
      generation and returned to control levels by the F3 generation. p,p'-DDE induced 
      a transgenerational impaired spermatogenesis with altered epigenetic 
      modification, posing significant implications for disease etiology.
CI  - Copyright (c) 2018 Elsevier B.V. All rights reserved.
FAU - Song, Yang
AU  - Song Y
AD  - Medical college, Hangzhou Normal University, No. 16 Xuelin Road, Xiasha Higher 
      Education Park, Hangzhou 310036, Zhejiang, PR China. Electronic address: 
      sygp_0@163.com.
FAU - Yang, Lei
AU  - Yang L
AD  - Medical college, Hangzhou Normal University, No. 16 Xuelin Road, Xiasha Higher 
      Education Park, Hangzhou 310036, Zhejiang, PR China. Electronic address: 
      yanglei62@hznu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20180825
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Endocrine Disruptors)
RN  - 0 (H19 long non-coding RNA)
RN  - 0 (Insecticides)
RN  - 0 (MEG3 non-coding RNA, rat)
RN  - 0 (RNA, Long Noncoding)
RN  - 4M7FS82U08 (Dichlorodiphenyl Dichloroethylene)
SB  - IM
MH  - Animals
MH  - DNA Methylation/drug effects
MH  - Dichlorodiphenyl Dichloroethylene/*toxicity
MH  - Endocrine Disruptors/*toxicity
MH  - Epigenesis, Genetic/drug effects
MH  - Female
MH  - Infertility, Male/chemically induced/genetics
MH  - Insecticides/*toxicity
MH  - Male
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects
MH  - RNA, Long Noncoding/*drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sperm Count
MH  - Spermatogenesis/*drug effects
MH  - Testis/pathology
OTO - NOTNLM
OT  - DNA methylation
OT  - Imprinted genes
OT  - Spermatogenesis
OT  - Transgeneration
OT  - p,p'-DDE
EDAT- 2018/08/29 06:00
MHDA- 2018/09/25 06:00
CRDT- 2018/08/29 06:00
PHST- 2018/03/30 00:00 [received]
PHST- 2018/06/12 00:00 [revised]
PHST- 2018/08/24 00:00 [accepted]
PHST- 2018/08/29 06:00 [pubmed]
PHST- 2018/09/25 06:00 [medline]
PHST- 2018/08/29 06:00 [entrez]
AID - S0378-4274(18)31813-7 [pii]
AID - 10.1016/j.toxlet.2018.08.015 [doi]
PST - ppublish
SO  - Toxicol Lett. 2018 Nov;297:34-41. doi: 10.1016/j.toxlet.2018.08.015. Epub 2018 
      Aug 25.