PMID- 30155025
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240327
IS  - 2041-6520 (Print)
IS  - 2041-6539 (Electronic)
IS  - 2041-6520 (Linking)
VI  - 7
IP  - 6
DP  - 2016 Jun 1
TI  - Fluorescence resonance energy transfer-based hybridization chain reaction for in 
      situ visualization of tumor-related mRNA.
PG  - 3829-3835
LID - 10.1039/c6sc00377j [doi]
AB  - The ability to visualize tumor-related mRNA in situ in single cells would 
      distinguish whether they are cancer cells or normal cells, which holds great 
      promise for cancer diagnosis at an early stage. Fluorescence resonance energy 
      transfer (FRET) and hybridization chain reactions (HCRs) were combined with 
      amplified sense tumor-related mRNA (TK1 mRNA) in situ with high sensitivity in 
      single cells and tissue sections. Using this strategy, each copy of the target 
      mRNA can propagate a chain reaction of hybridization events between two 
      alternating hairpins to form a nicked duplex that contains repeated FRET units, 
      amplifying the fluorescent signal. The detection limit of 18 pM is about three 
      orders of magnitude lower than that of a non-HCR method (such as the 
      binary-probe-system). Meanwhile, due to the FRET strategy, complicated washing 
      steps are not necessary and experimental time is sharply reduced. As far as we 
      know, this is the first report of a fluorescence in situ hybridization (FISH) 
      strategy that can simultaneously fulfil signal amplification and is wash-free. We 
      believe that this FRET-based HCR strategy has great potential as a powerful tool 
      in basic research and clinical diagnosis.
FAU - Huang, Jin
AU  - Huang J
AD  - State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry 
      and Chemical Engineering , Key Laboratory for Bio-Nanotechnology and Molecular 
      Engineering of Hunan Province , Hunan University , Changsha , P. R. China . 
      Email: kmwang@hnu.edu.cn.
FAU - Wang, He
AU  - Wang H
AD  - State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry 
      and Chemical Engineering , Key Laboratory for Bio-Nanotechnology and Molecular 
      Engineering of Hunan Province , Hunan University , Changsha , P. R. China . 
      Email: kmwang@hnu.edu.cn.
FAU - Yang, Xiaohai
AU  - Yang X
AD  - State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry 
      and Chemical Engineering , Key Laboratory for Bio-Nanotechnology and Molecular 
      Engineering of Hunan Province , Hunan University , Changsha , P. R. China . 
      Email: kmwang@hnu.edu.cn.
FAU - Quan, Ke
AU  - Quan K
AD  - State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry 
      and Chemical Engineering , Key Laboratory for Bio-Nanotechnology and Molecular 
      Engineering of Hunan Province , Hunan University , Changsha , P. R. China . 
      Email: kmwang@hnu.edu.cn.
FAU - Yang, Yanjing
AU  - Yang Y
AD  - State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry 
      and Chemical Engineering , Key Laboratory for Bio-Nanotechnology and Molecular 
      Engineering of Hunan Province , Hunan University , Changsha , P. R. China . 
      Email: kmwang@hnu.edu.cn.
FAU - Ying, Le
AU  - Ying L
AD  - State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry 
      and Chemical Engineering , Key Laboratory for Bio-Nanotechnology and Molecular 
      Engineering of Hunan Province , Hunan University , Changsha , P. R. China . 
      Email: kmwang@hnu.edu.cn.
FAU - Xie, Nuli
AU  - Xie N
AD  - State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry 
      and Chemical Engineering , Key Laboratory for Bio-Nanotechnology and Molecular 
      Engineering of Hunan Province , Hunan University , Changsha , P. R. China . 
      Email: kmwang@hnu.edu.cn.
FAU - Ou, Min
AU  - Ou M
AD  - State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry 
      and Chemical Engineering , Key Laboratory for Bio-Nanotechnology and Molecular 
      Engineering of Hunan Province , Hunan University , Changsha , P. R. China . 
      Email: kmwang@hnu.edu.cn.
FAU - Wang, Kemin
AU  - Wang K
AD  - State Key Laboratory of Chemo/Biosensing and Chemometrics , College of Chemistry 
      and Chemical Engineering , Key Laboratory for Bio-Nanotechnology and Molecular 
      Engineering of Hunan Province , Hunan University , Changsha , P. R. China . 
      Email: kmwang@hnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160225
PL  - England
TA  - Chem Sci
JT  - Chemical science
JID - 101545951
PMC - PMC6013822
EDAT- 2016/06/01 00:00
MHDA- 2016/06/01 00:01
PMCR- 2016/02/25
CRDT- 2018/08/30 06:00
PHST- 2016/01/26 00:00 [received]
PHST- 2016/02/25 00:00 [accepted]
PHST- 2018/08/30 06:00 [entrez]
PHST- 2016/06/01 00:00 [pubmed]
PHST- 2016/06/01 00:01 [medline]
PHST- 2016/02/25 00:00 [pmc-release]
AID - c6sc00377j [pii]
AID - 10.1039/c6sc00377j [doi]
PST - ppublish
SO  - Chem Sci. 2016 Jun 1;7(6):3829-3835. doi: 10.1039/c6sc00377j. Epub 2016 Feb 25.