PMID- 30155683
OWN - NLM
STAT- MEDLINE
DCOM- 20190220
LR  - 20200225
IS  - 1476-3524 (Electronic)
IS  - 1029-8428 (Print)
IS  - 1029-8428 (Linking)
VI  - 35
IP  - 1
DP  - 2019 Jan
TI  - Blebbistatin, a Myosin II Inhibitor, Exerts Antidepressant-Like Activity and 
      Suppresses Detrusor Overactivity in an Animal Model of Depression Coexisting with 
      Overactive Bladder.
PG  - 196-207
LID - 10.1007/s12640-018-9948-5 [doi]
AB  - Overactive bladder (OAB) coexists with depression in women. Here, we assessed the 
      effects of a 1-week treatment with blebbistatin, a myosin II inhibitor, on 
      changes in behavior and detrusor overactivity (DO) symptoms induced by a 6-week 
      administration of 13-cis-retinoic acid (13-cis-RA), with the aid of the forced 
      swim test (FST), spontaneous locomotor activity test, and in vivo cystometric 
      investigations in female Wistar rats. 13-cis-RA-induced depressive-like behavior 
      and DO symptoms were associated with increased corticotropin-releasing factor 
      (CRF) level in the plasma, prefrontal cortex (PFC), hippocampus (Hp), 
      Barrington's nucleus (BN), and urinary bladder. Moreover, 13-cis-RA decreased 
      brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) levels in 
      plasma, PFC, Hp, and BN, while it increased BDNF and NGF levels in urinary 
      bladder. Blebbistatin exerted antidepressant-like effect and attenuated changes 
      in the cystometric parameters as well as the central and peripheral levels of 
      CRF, BDNF, and NGF that were induced by 13-cis-RA, while it did not affect urine 
      production, mean, systolic or diastolic blood pressure, or heart rate. The 
      results point to blebbistatin as a potential treatment option for OAB coexisting 
      with depression.
FAU - Wrobel, Andrzej
AU  - Wrobel A
AD  - Second Department of Gynecology, Medical University of Lublin, Jaczewskiego 8, 
      20-090, Lublin, Poland. wrobelandrzej@yahoo.com.
FAU - Doboszewska, Urszula
AU  - Doboszewska U
AD  - Department of Animal Physiology, Institute of Biology and Biochemistry, Faculty 
      of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19, 
      20-033, Lublin, Poland. urszula.doboszewska@umcs.lublin.pl.
FAU - Rechberger, Ewa
AU  - Rechberger E
AD  - Second Department of Gynecology, Medical University of Lublin, Jaczewskiego 8, 
      20-090, Lublin, Poland.
FAU - Banczerowska-Gorska, Malgorzata
AU  - Banczerowska-Gorska M
AD  - Gynaecological and Obstetrics Hospital in Walbrzych, Paderewskiego 10, 58-301, 
      Walbrzych, Poland.
FAU - Czuczwar, Piotr
AU  - Czuczwar P
AD  - Third Department of Gynecology, Medical University of Lublin, Jaczewskiego 8, 
      20-954, Lublin, Poland.
FAU - Poleszak, Ewa
AU  - Poleszak E
AD  - Chair and Department of Applied Pharmacy, Medical University of Lublin, Chodzki 
      1, 20-093, Lublin, Poland.
FAU - Dudka, Jaroslaw
AU  - Dudka J
AD  - Chair and Department of Toxicology, Medical University of Lublin, Chodzki 8, 
      20-093, Lublin, Poland.
FAU - Wlaz, Piotr
AU  - Wlaz P
AD  - Department of Animal Physiology, Institute of Biology and Biochemistry, Faculty 
      of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19, 
      20-033, Lublin, Poland.
FAU - Miotla, Pawel
AU  - Miotla P
AD  - Second Department of Gynecology, Medical University of Lublin, Jaczewskiego 8, 
      20-090, Lublin, Poland.
FAU - Wlazlak, Edyta
AU  - Wlazlak E
AD  - Clinic of Operative Gynecology and Gynecologic Oncology, 1st Department of 
      Gynecology and Obstetrics, Medical University of Lodz, Wilenska 37, 94-029, Lodz, 
      Poland.
FAU - Rechberger, Tomasz
AU  - Rechberger T
AD  - Second Department of Gynecology, Medical University of Lublin, Jaczewskiego 8, 
      20-090, Lublin, Poland.
LA  - eng
PT  - Journal Article
DEP - 20180828
PL  - United States
TA  - Neurotox Res
JT  - Neurotoxicity research
JID - 100929017
RN  - 0 (Antidepressive Agents)
RN  - 0 (Autonomic Agents)
RN  - 0 (Bdnf protein, rat)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Heterocyclic Compounds, 4 or More Rings)
RN  - 20WC4J7CQ6 (blebbistatin)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 3.6.1.- (Myosin Type II)
RN  - EH28UP18IF (Isotretinoin)
SB  - IM
MH  - Animals
MH  - Antidepressive Agents/*pharmacology
MH  - Autonomic Agents/*pharmacology
MH  - Brain/metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Corticotropin-Releasing Hormone/metabolism
MH  - Depression/complications/*drug therapy
MH  - Disease Models, Animal
MH  - Female
MH  - Heterocyclic Compounds, 4 or More Rings/*pharmacology
MH  - Isotretinoin
MH  - Myosin Type II/antagonists & inhibitors/metabolism
MH  - Nerve Growth Factor/metabolism
MH  - Random Allocation
MH  - Rats, Wistar
MH  - Urinary Bladder/*drug effects/physiopathology
MH  - Urinary Bladder, Overactive/complications/*drug therapy
PMC - PMC6313360
OTO - NOTNLM
OT  - 13-cis-Retinoic acid
OT  - Blebbistatin
OT  - Forced swim test
OT  - Overactive bladder
OT  - Rats
COIS- The authors declare that they have no conflict of interest.
EDAT- 2018/08/30 06:00
MHDA- 2019/03/21 06:00
PMCR- 2018/08/28
CRDT- 2018/08/30 06:00
PHST- 2018/06/06 00:00 [received]
PHST- 2018/08/15 00:00 [accepted]
PHST- 2018/08/13 00:00 [revised]
PHST- 2018/08/30 06:00 [pubmed]
PHST- 2019/03/21 06:00 [medline]
PHST- 2018/08/30 06:00 [entrez]
PHST- 2018/08/28 00:00 [pmc-release]
AID - 10.1007/s12640-018-9948-5 [pii]
AID - 9948 [pii]
AID - 10.1007/s12640-018-9948-5 [doi]
PST - ppublish
SO  - Neurotox Res. 2019 Jan;35(1):196-207. doi: 10.1007/s12640-018-9948-5. Epub 2018 
      Aug 28.