PMID- 30155908
OWN - NLM
STAT- MEDLINE
DCOM- 20190123
LR  - 20190123
IS  - 1600-0560 (Electronic)
IS  - 0303-6987 (Linking)
VI  - 45
IP  - 12
DP  - 2018 Dec
TI  - Review of the medical literature and assessment of current utilization patterns 
      regarding the use of two common fluorescence in situ hybridization assays in the 
      diagnosis of dermatofibrosarcoma protuberans and clear cell sarcoma.
PG  - 905-913
LID - 10.1111/cup.13345 [doi]
AB  - BACKGROUND: Dermatofibrosarcoma protuberans (DFSP) is a tumor of intermediate 
      malignancy, which in selected circumstances can pose difficulty in diagnosis. 
      Clear cell sarcoma (CCS) is a very rare aggressive soft tissue sarcoma that can 
      be difficult to distinguish histologically from melanoma. METHODS: The current 
      literature on t(17;22) COL1A1-PDGFB fluorescence in situ hybridization (FISH) 
      assay in DFSP was reviewed. Also reviewed was the current literature on dual 
      color break-apart EWSR1 FISH assay in CCS. Finally, the current utilization 
      patterns of these tests was assessed in attendees of the American Society of 
      Dermatopathology annual meeting (Chicago, 2016). RESULTS: The literature 
      indicates that (17;22) COL1A1-PDGFB FISH assay has limited value for classic 
      DFSP, where the diagnosis can be established by routine morphology and 
      immunohistochemistry. Given the high specificity of the EWSR1 FISH assay and 
      significant complexity in the diagnosis of CCS, this ancillary study is helpful 
      in distinguishing CCS from melanoma. CONCLUSIONS: In attendees, t(17;22) 
      COL1A1-PDGFB FISH testing for classic cases of DFSP is appropriately not being 
      used by respondents. However, the literature sustains that it is useful in 
      selected circumstances in which a definitive diagnosis is challenging. The 
      majority of respondents are utilizing the EWSR1 FISH assay to distinguish CSS 
      from melanoma as is supported by the literature.
CI  - (c) 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Linos, Konstantinos
AU  - Linos K
AUID- ORCID: 0000-0001-9462-652X
AD  - Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical 
      Center and Geisel School of Medicine at Dartmouth Lebanon, Lebanon, New 
      Hampshire.
FAU - Kozel, Jessica A
AU  - Kozel JA
AD  - Midwest Pathology Associates, LLC, Overland Park, Kansas.
FAU - Hurley, Maria Yadira
AU  - Hurley MY
AUID- ORCID: 0000-0002-1162-7250
AD  - Departments of Dermatology and Pathology, Saint Louis University School of 
      Medicine, St. Louis, Missouri.
FAU - Andea, Aleodor A
AU  - Andea AA
AD  - Departments of Dermatology and Pathology, University of Michigan Medical Center, 
      Ann Arbor, Michigan.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20180927
PL  - United States
TA  - J Cutan Pathol
JT  - Journal of cutaneous pathology
JID - 0425124
SB  - IM
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Chromosomes, Human, Pair 22/*genetics
MH  - *Dermatofibrosarcoma/diagnosis/genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Sarcoma, Clear Cell/diagnosis/genetics/pathology
MH  - *Skin Neoplasms/diagnosis/genetics/pathology
MH  - *Translocation, Genetic
OTO - NOTNLM
OT  - EWSR1
OT  - clear cell sarcoma
OT  - dermatofibrosarcoma protuberans
OT  - fluorescence in situ hybridization
OT  - t(17;22) COL1A1-PDGFB
EDAT- 2018/08/30 06:00
MHDA- 2019/01/24 06:00
CRDT- 2018/08/30 06:00
PHST- 2017/10/09 00:00 [received]
PHST- 2018/07/14 00:00 [revised]
PHST- 2018/08/06 00:00 [accepted]
PHST- 2018/08/30 06:00 [pubmed]
PHST- 2019/01/24 06:00 [medline]
PHST- 2018/08/30 06:00 [entrez]
AID - 10.1111/cup.13345 [doi]
PST - ppublish
SO  - J Cutan Pathol. 2018 Dec;45(12):905-913. doi: 10.1111/cup.13345. Epub 2018 Sep 
      27.