PMID- 30156389
OWN - NLM
STAT- MEDLINE
DCOM- 20190826
LR  - 20190826
IS  - 1899-5276 (Print)
IS  - 1899-5276 (Linking)
VI  - 28
IP  - 1
DP  - 2019 Jan
TI  - Vitamin C may exert variable effects on viability and proliferation of HeLa cells 
      exhibiting high and low chromosomal instability.
PG  - 19-24
LID - 10.17219/acem/76870 [doi]
AB  - BACKGROUND: Chromosomal instability (CIN), defined as abnormality in chromosome 
      structure or number, is the hallmark of malignancies. The role of vitamin C in 
      cancer treatment is controversial and its effect on CIN is still an open field of 
      research. In this work, we tried to study the effect of high-dose L-ascorbic acid 
      (L-AA) on CIN-induced (CINhi = CIN high) and non-CIN-induced (CINlo = CIN low) 
      cervical cancer cells. OBJECTIVES: The aim of this study was to explore the 
      effect of high-dose L-AA on CIN in the cervical cancer cell line (HeLa) cells. 
      MATERIAL AND METHODS: The HeLa cells (CINhi and CINlo) were treated with 2 doses 
      (5 mM and 8 mM) of L-AA for 24 h and 48 h. They were then analyzed by 
      micronucleus (MN) scoring, cell ploidy and flow cytometry, the latter regarding 
      gammaH2AX expression. Cell viability was assessed by the methylthiazol tetrazolium 
      (MTT) and Annexin V assays. RESULTS: Treatment of CINhi cells with L-AA led to a 
      decrease in MN score (colchicine - 71.5 +/-4.95, 67.5 +/-0.71; L-AA (5 mM) - 49 
      +/-7.07, 46.5 +/-4.95; L-AA (8 mM) - 42 +/-9.89, 41 +/-1.41, at 24 h and 48 h, 
      respectively; p < 0.05). Treatment of CINlo cells with L-AA resulted in increased 
      MN score (5 mM - 45 +/-7.07, 36 +/-4.24; 8 mM - 34.5 +/-4.95, 31 +/-1.41, at 24 h and 48 
      h, respectively; control - 15.5 +/-0.71, 12.5 +/-0.71; p < 0.05) and reduction in 
      cancer cell viability (control - 100%; L-AA (5 mM) - 76.32% +/-28.73, 72.74% 
      +/-20.30; L-AA (8 mM) - 66.14% +/-19.13, 66.99% +/-19.99, at 24 h and 48 h, 
      respectively; p < 0.05). The expression of gammaH2AX was high in both groups at 48 h 
      (mean CINhi = 19.42%, CINlo = 21.14%; control = 1.19% and 1.58%, respectively) 
      with the 8 mM dose of L-AA. CONCLUSIONS: L-ascorbic acid was found to have a 
      differential effect on CINhi and CINlo HeLa cells, which may be due to 
      differences in oxidation status of these 2 categories.
FAU - Sindhwani, Aastha
AU  - Sindhwani A
AD  - Department of Experimental Medicine & Biotechnology, Postgraduate Institute of 
      Medical Education & Research, Chandigarh, India.
FAU - Muthusammy, Sasikala
AU  - Muthusammy S
AD  - Department of Experimental Medicine & Biotechnology, Postgraduate Institute of 
      Medical Education & Research, Chandigarh, India.
FAU - Bhatia, Alka
AU  - Bhatia A
AD  - Department of Experimental Medicine & Biotechnology, Postgraduate Institute of 
      Medical Education & Research, Chandigarh, India.
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Adv Clin Exp Med
JT  - Advances in clinical and experimental medicine : official organ Wroclaw Medical 
      University
JID - 101138582
RN  - PQ6CK8PD0R (Ascorbic Acid)
MH  - Ascorbic Acid/administration & dosage/*pharmacology
MH  - Cell Proliferation
MH  - *Chromosomal Instability
MH  - Female
MH  - HeLa Cells/*drug effects
MH  - Humans
MH  - *Uterine Cervical Neoplasms/genetics/pathology
OTO - NOTNLM
OT  - cancer
OT  - chromosomal instability
OT  - vitamin C
EDAT- 2018/08/30 06:00
MHDA- 2019/08/27 06:00
CRDT- 2018/08/30 06:00
PHST- 2018/08/30 06:00 [pubmed]
PHST- 2019/08/27 06:00 [medline]
PHST- 2018/08/30 06:00 [entrez]
AID - 10.17219/acem/76870 [doi]
PST - ppublish
SO  - Adv Clin Exp Med. 2019 Jan;28(1):19-24. doi: 10.17219/acem/76870.