PMID- 30157106
OWN - NLM
STAT- MEDLINE
DCOM- 20190814
LR  - 20200930
IS  - 1530-0315 (Electronic)
IS  - 0195-9131 (Linking)
VI  - 51
IP  - 1
DP  - 2019 Jan
TI  - Brain-derived Neurotrophic Factor Pathway after Downhill and Uphill Training in 
      Rats.
PG  - 27-34
LID - 10.1249/MSS.0000000000001771 [doi]
AB  - INTRODUCTION: The elevation of brain-derived neurotrophic factor (BDNF) levels in 
      the brain and the subsequent phosphorylation of its cognate tropomyosin-related 
      kinase B (TrkB) receptors at tyrosine 816 (pTrkB) are largely involved in the 
      positive effect of aerobic exercise on brain functioning. Although BDNF levels 
      were reported to increase in proportion with exercise intensity, the effect of 
      the type of contraction is unknown. Therefore, the cerebral BDNF/TrkB pathway was 
      investigated after uphill and downhill treadmill activities at equivalent 
      intensity to preferentially induce eccentric and concentric contractions, 
      respectively. METHODS: A treadmill activity (30 min.d for seven consecutive days) 
      either in a horizontal position at two different speeds to modulate intensity 
      (experiment 1) or at three different inclinations (null, -10%, and +5%) but at 
      equivalent intensity to modulate the type of contraction (experiment 2) was 
      induced in rats. Both experiments included sedentary rats. Levels of BDNF, pTrkB, 
      synaptophysin (marker of synaptogenesis), endothelial nitric oxide synthase 
      phosphorylated at serine 1177 (peNOS), and c-fos levels (indicators of elevation 
      in blood flow in the cerebrovasculature and neuronal activity, respectively) were 
      measured in motor- and cognition-related brain regions using Western blotting 
      analysis. RESULTS: Experiment 1 indicated that treadmill activity induces an 
      intensity-dependent increase in peNOS, c-fos, and BDNF levels. Experiment 2 
      showed that intensity of exercise as well as activation of the cerebral BDNF 
      pathway, and synaptogenesis did not differ among horizontal, uphill, and downhill 
      treadmill activities. CONCLUSION: The cerebral response of the BDNF pathway to a 
      treadmill activity is dependent on exercise intensity, but not on the type of 
      contraction (eccentric vs concentric).
FAU - Pedard, Martin
AU  - Pedard M
AD  - INSERM U1093 CAPS, Burgundy and Franche-Comte University, Dijon, FRANCE.
AD  - Department of Neurology, Regional University Hospital Center of Dijon, Dijon, 
      FRANCE.
FAU - Cefis, Marina
AU  - Cefis M
AD  - INSERM U1093 CAPS, Burgundy and Franche-Comte University, Dijon, FRANCE.
FAU - Ennequin, Gael
AU  - Ennequin G
AD  - EA4267 PEPITE, (EPSI), Burgundy and Franche-Comte University, Besancon, FRANCE.
FAU - Quirie, Aurore
AU  - Quirie A
AD  - INSERM U1093 CAPS, Burgundy and Franche-Comte University, Dijon, FRANCE.
FAU - Garnier, Philippe
AU  - Garnier P
AD  - INSERM U1093 CAPS, Burgundy and Franche-Comte University, Dijon, FRANCE.
FAU - Prigent-Tessier, Anne
AU  - Prigent-Tessier A
AD  - INSERM U1093 CAPS, Burgundy and Franche-Comte University, Dijon, FRANCE.
FAU - Pernet, Nicolas
AU  - Pernet N
AD  - INSERM U1093 CAPS, Burgundy and Franche-Comte University, Dijon, FRANCE.
FAU - Marie, Christine
AU  - Marie C
AD  - INSERM U1093 CAPS, Burgundy and Franche-Comte University, Dijon, FRANCE.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Med Sci Sports Exerc
JT  - Medicine and science in sports and exercise
JID - 8005433
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (Synaptophysin)
RN  - 0 (Syp protein, rat)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.13.39 (Nos3 protein, rat)
RN  - EC 2.7.10.1 (Ntrk2 protein, rat)
RN  - EC 2.7.10.1 (Receptor, trkB)
MH  - Animals
MH  - Brain/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Muscle Contraction/physiology
MH  - Nitric Oxide Synthase Type III/metabolism
MH  - Phosphorylation
MH  - Physical Conditioning, Animal/*methods/physiology
MH  - Proto-Oncogene Proteins c-fos/metabolism
MH  - Rats, Wistar
MH  - Receptor, trkB/metabolism
MH  - Synaptophysin/metabolism
EDAT- 2018/08/30 06:00
MHDA- 2019/08/15 06:00
CRDT- 2018/08/30 06:00
PHST- 2018/08/30 06:00 [pubmed]
PHST- 2019/08/15 06:00 [medline]
PHST- 2018/08/30 06:00 [entrez]
AID - 10.1249/MSS.0000000000001771 [doi]
PST - ppublish
SO  - Med Sci Sports Exerc. 2019 Jan;51(1):27-34. doi: 10.1249/MSS.0000000000001771.