PMID- 30158539 OWN - NLM STAT- MEDLINE DCOM- 20191120 LR - 20191120 IS - 2041-4889 (Electronic) VI - 9 IP - 9 DP - 2018 Aug 29 TI - Human menstrual blood-derived stem cells promote functional recovery in a rat spinal cord hemisection model. PG - 882 LID - 10.1038/s41419-018-0847-8 [doi] LID - 882 AB - Spinal cord injury (SCI) is associated with a dismal prognosis including severe voluntary motor and sensory deficits in the presence of the current therapies, thus new and efficient treatment strategies are desperately required. Along with several advantages, such as easy accessibility, high-yield, potential of enormous proliferation, menstrual blood-derived mesenchymal stem cells (MenSCs) have been proposed as a promising strategy in regeneration medicine. In this study, the MenSCs were transplanted into incomplete thoracic (T10) spinal cord injury (SCI) rats, all rats were sacrificed at 7, 14, and 28 days after surgery. Based on the results, we found that MenSCs transplantation improved the hind limb motor function. Besides, H&E staining showed that MenSCs treatment markedly reduced cavity formation in the lesion site. Furthermore, treatment by MenSCs showed more MAP2-positive mature neurons, as well as axonal regeneration manifested by NF-200 and less expression of chondroitin sulfate proteoglycans (CSPGs) than the non-treatment in the lesion site. Additionally, immunofluorescence, Western blot, and qRT-PCR methods showed that levels of brain-derived neurotrophic factor (BDNF) were significantly higher in the injured spinal cord after implantation of MenSCs. Results of qRT-PCR indicated that inflammatory factors, including TNF-alpha and IL-1beta were inhibited after MenSCs transplantation. The improved motor function of hind limb and the increased cell body area of motor neurons were suppressed by blocking of the BDNF-TrkB signaling. It was eventually revealed that MenSCs implantation had beneficial therapeutic effects on the rehabilitation of the rat spinal cord hemisection model, mainly by enhancing the expression of BDNF. MenSCs transplantation may provide a novel therapeutic strategy for patients with SCI in the future. FAU - Wu, Qinfeng AU - Wu Q AD - Department of Anatomy, Medical School of Nantong University, Laboratory Animal Center of Nantong University, Nantong, Jiangsu Province, 226001, China. AD - Department of Rehabilitation Medicine, Suzhou Hospital affiliated to Nanjing Medical University, Suzhou Science & Technology Town Hospital, 215153, Suzhou, Jiangsu Province, China. FAU - Wang, Qinghua AU - Wang Q AD - Department of Anatomy, Medical School of Nantong University, Laboratory Animal Center of Nantong University, Nantong, Jiangsu Province, 226001, China. FAU - Li, Zhangjie AU - Li Z AD - Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu Province, China. AD - Department of Rehabilitation Medicine, Zhangjiagang First People's Hospital, 215600, Zhangjiagang, Jiangsu Province, China. FAU - Li, Xiangzhe AU - Li X AD - Department of Rehabilitation Medicine, Suzhou Hospital affiliated to Nanjing Medical University, Suzhou Science & Technology Town Hospital, 215153, Suzhou, Jiangsu Province, China. FAU - Zang, Jing AU - Zang J AD - Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu Province, China. FAU - Wang, Zhangwei AU - Wang Z AD - Department of Anatomy, Medical School of Nantong University, Laboratory Animal Center of Nantong University, Nantong, Jiangsu Province, 226001, China. FAU - Xu, Chen AU - Xu C AD - Department of Anatomy, Medical School of Nantong University, Laboratory Animal Center of Nantong University, Nantong, Jiangsu Province, 226001, China. FAU - Gong, Yujia AU - Gong Y AD - Department of Anatomy, Medical School of Nantong University, Laboratory Animal Center of Nantong University, Nantong, Jiangsu Province, 226001, China. FAU - Cheng, Jiaqi AU - Cheng J AD - Department of Anatomy, Medical School of Nantong University, Laboratory Animal Center of Nantong University, Nantong, Jiangsu Province, 226001, China. FAU - Li, Haoming AU - Li H AD - Department of Anatomy, Medical School of Nantong University, Laboratory Animal Center of Nantong University, Nantong, Jiangsu Province, 226001, China. FAU - Shen, Guangyu AU - Shen G AD - Department of Rehabilitation Medicine, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu Province, China. FAU - Dong, Chuanming AU - Dong C AD - Department of Anatomy, Medical School of Nantong University, Laboratory Animal Center of Nantong University, Nantong, Jiangsu Province, 226001, China. yiyimarket@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20180829 PL - England TA - Cell Death Dis JT - Cell death & disease JID - 101524092 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Interleukin-1beta) RN - 0 (Membrane Glycoproteins) RN - 0 (Tumor Necrosis Factor-alpha) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/metabolism MH - Cells, Cultured MH - Female MH - Humans MH - Inflammation/metabolism/pathology MH - Interleukin-1beta/metabolism MH - Membrane Glycoproteins/metabolism MH - Mesenchymal Stem Cell Transplantation/methods MH - Mesenchymal Stem Cells/metabolism/*pathology MH - Nerve Regeneration/physiology MH - Neurons/metabolism/pathology MH - Rats MH - Rats, Sprague-Dawley MH - Recovery of Function/physiology MH - Spinal Cord/*metabolism/*pathology MH - Spinal Cord Injuries/*metabolism/*pathology MH - Stem Cells/*metabolism/pathology MH - Tumor Necrosis Factor-alpha/metabolism PMC - PMC6115341 COIS- The authors declare that they have no conflict of interest. EDAT- 2018/08/31 06:00 MHDA- 2019/11/21 06:00 PMCR- 2018/08/29 CRDT- 2018/08/31 06:00 PHST- 2018/01/15 00:00 [received] PHST- 2018/06/27 00:00 [accepted] PHST- 2018/06/25 00:00 [revised] PHST- 2018/08/31 06:00 [entrez] PHST- 2018/08/31 06:00 [pubmed] PHST- 2019/11/21 06:00 [medline] PHST- 2018/08/29 00:00 [pmc-release] AID - 10.1038/s41419-018-0847-8 [pii] AID - 847 [pii] AID - 10.1038/s41419-018-0847-8 [doi] PST - epublish SO - Cell Death Dis. 2018 Aug 29;9(9):882. doi: 10.1038/s41419-018-0847-8.