PMID- 30168910 OWN - NLM STAT- MEDLINE DCOM- 20190408 LR - 20190408 IS - 1934-340X (Electronic) IS - 1934-3396 (Linking) VI - 63 IP - 1 DP - 2018 Sep TI - Installing, Maintaining, and Using a Local Copy of BLAST for Compute Cluster or Workstation Use. PG - e54 LID - 10.1002/cpbi.54 [doi] AB - The Basic Local Alignment Search Tool (BLAST) is the first resource to computationally characterize a novel amino acid or nucleic acid sequence. BLAST plays important roles in genomics, transcriptomics, and protein science. For numerous academic and commercial researchers, neither BLAST Web servers nor cloud resources satisfy the requirements of high-throughput comparative genomic pipelines or company policies. For such users, this unit describes how to install BLAST locally, either on a standalone workstation, or preferably on a compute cluster. We provide practical guidance for the planning and the installation under the LINUX, Windows, and Mac OS X operating systems. We propose strategies for downloading existing and generating new sequence databases in BLAST format. (c) 2018 by John Wiley & Sons, Inc. CI - (c) 2018 John Wiley & Sons, Inc. FAU - Ladunga, Istvan AU - Ladunga I AD - Departments of Statistics, Biochemistry, and School of Biological Sciences, University of Nebraska-Lincoln, Lincoln, Nebraska. LA - eng PT - Journal Article DEP - 20180831 PL - United States TA - Curr Protoc Bioinformatics JT - Current protocols in bioinformatics JID - 101157830 SB - IM MH - *Algorithms MH - Databases, Nucleic Acid MH - Sequence Alignment/*methods OTO - NOTNLM OT - BLAST OT - LINUX OT - biosequence analysis OT - sequence similarity search EDAT- 2018/09/01 06:00 MHDA- 2019/04/09 06:00 CRDT- 2018/09/01 06:00 PHST- 2018/09/01 06:00 [pubmed] PHST- 2019/04/09 06:00 [medline] PHST- 2018/09/01 06:00 [entrez] AID - 10.1002/cpbi.54 [doi] PST - ppublish SO - Curr Protoc Bioinformatics. 2018 Sep;63(1):e54. doi: 10.1002/cpbi.54. Epub 2018 Aug 31.